EAGER: Estimating the prevalence of polyploidy in the phaseoloid legumes

EAGER：估计菜豆中多倍体的流行率

• 批准号: 0948800
• 负责人: Jeffrey Doyle
• 金额: $ 24.57万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0948800&HistoricalAwards=false
• 关键词: EAGER; Estimating; prevalence; polyploidy; phaseoloid

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Polyploidy (whole genome duplication) is one of the most important genetic phenomena in plants. Immediately after its formation, a polyploid plant can be identified simply by counting its chromosomes, because it has twice as many as a diploid. However, after millions of years a polyploid may have as low a chromosome number as a diploid through a process called "diploidization." Genomic studies can detect such cryptic polyploids, and the advent of high-throughput sequencing has made this approach increasingly feasible. Using this new technology, this project will estimate the frequency of polyploidy in the phaseoloid legumes, a group that includes soybean, common bean, cowpea, pigeonpea, and other less familiar crops, by sequencing thousands of transcribed genes from approximately 25 phylogenetically diverse species. Polyploid events will be identified by their characteristic signature, an excess of genes with two copies duplicated at the same time. The project has broad significance for genetics and systematics, given the importance of polyploidy as a genetic phenomenon, and also for agriculture, because polyploidy is so common among crop plants (e.g., soybean, wheat, maize, cotton). The large numbers of genes will be a resource for subsequent studies in this large and important lineage. A talented female postdoctoral associate will receive training in the generation and analysis of next-generation sequencing data.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。多倍体（全基因组复制）是植物中最重要的遗传现象之一。多倍体植物形成后，可以通过简单地计数其染色体来鉴定，因为它的染色体数是二倍体的两倍。然而，经过数百万年的多倍体可能有一个低的染色体数目作为一个二倍体通过一个过程称为“二倍体化”。“基因组研究可以检测到这种隐藏的多倍体，高通量测序的出现使这种方法变得越来越可行。利用这项新技术，该项目将通过对来自大约25个遗传多样性物种的数千个转录基因进行测序，来估计菜豆类豆类（包括大豆、菜豆、豇豆、木豆和其他不太熟悉的作物）中多倍体的频率。多倍体事件将通过它们的特征标记来识别，即同时复制两个拷贝的过量基因。鉴于多倍性作为一种遗传现象的重要性，该项目对遗传学和系统学具有广泛的意义，也对农业具有广泛的意义，因为多倍性在作物植物中是如此普遍（例如，大豆、小麦、玉米、棉花）。大量的基因将为这一庞大而重要的谱系的后续研究提供资源。一位才华横溢的女博士后将接受下一代测序数据生成和分析方面的培训。