Molecular Approaches to the Phylogeny of Leguminosae

豆科植物是开花植物的第三大科，包括许多具有重要生态和经济价值的物种。为了理解这种巨大的跳跃，有必要描述将许多物种和属彼此联系在一起的关系和系统发育模式。这种类型的许多工作已经完成，并将继续使用传统的字符，如形态和化学。这项研究将集中在DNA水平上的变异模式，特别是强调叶绿体基因组，它已被证明是构建系统发育假说的特征来源的重要来源。限制性内切酶切点分析和DNA测序将被用来调查代表关键亚科Papilionoideae和Caesalpinioideae的所有主要部落的属，这两个组被认为代表了该科的主要辐射。这些研究的结果将与社区其他地方正在进行的非分子研究的结果相结合。由此产生的系统发育假说将用于研究该家族固氮共生体的起源，重点是参与结瘤的核心多基因家族的发展。

Leguminosae is the third largest family of flowering plants, and includes many ecologically and economically important species. To understand this great divesity it is necessary to describe the patterns of relationship and phylogeny linking the many species and genera to one another. Much work of this type has been done, and continues, using traditional characters such as morphology and chemistry. This study will focus on variation patterns at the DNA level, specifically emphasizing the chloroplast genome, which has proven to be of great utility as a source of characters for constructing phylogenetic hypotheses. Restriction site analysis and DNA sequencing will be used to survey genera representing all of the major tribes of the pivotal subfamilies Papilionoideae and Caesalpinioideae, the groups considered to represent the main radiation of the family. Results from these studies will be integrated with those of non-molecular studies progressing elsewhere in the community. The resulting phylogenetic hypotheses will be used to study the origins of nitrogen-fixing symbioses in the family, with emphasis on the development of the nuclear multigene families involved in nodulation.