RUI: Interdependence of Nutrient and Pheromone Sensing Pathways in Yeast

RUI：酵母中营养物质和信息素传感途径的相互依赖性

• 批准号: 0952519
• 负责人: Stephen Wright
• 金额: $ 43.91万
• 依托单位: Maryville College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2013-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0952519&HistoricalAwards=false
• 关键词: RUI; Interdependence; Nutrient; Pheromone; Sensing

Cell division and fusion are resource intensive activities, and thus the ability of the yeast cell to coordinate the availability of nutrients in its environment with reproduction is of great physiological importance. This project will examine the role that glucose availability plays in yeast mating by testing the hypothesis that glucose sensing and mating are interdependent. The glucose sensor and the pheromone receptor are the only G protein coupled receptors (GPCR) in yeast. The aims of this project include 1) assessing the effects of pheromone stimulation on the glucose sensor, 2) examining the direct physical interactions of the glucose sensing and pheromone receptors, and 3) identifying interactions between components of the signaling pathways downstream of these receptors. Broader Impacts: This project, while enhancing our understanding of the relationship between nutrient sensing and mating in yeast, will impact undergraduate students and the science faculty at three primarily undergraduate institutions. Undergraduate students will be exposed to biochemical research during an intensive ten week summer project, with the opportunity to continue their research during the academic year. These students will be involved in the design, implementation, interpretation, and dissemination of the results of their own experiments in a community of their peers. This project represents an opportunity for the investigators to integrate their research into their teaching, thereby enhancing the scientific education of hundreds of students at the participating institutions. The students involved in this research will be primarily from a typically underserved population (including under-represented minorities) in rural Appalachia.

细胞分裂和融合是资源密集的活动，因此，酵母细胞与繁殖环境中养分的可用性协调的能力非常重要。 该项目将通过测试葡萄糖感应和交配是相互依存的假设来研究葡萄糖可用性在酵母交配中起作用的作用。 葡萄糖传感器和信息素受体是酵母中唯一的G蛋白偶联受体（GPCR）。该项目的目的包括1）评估信息素刺激对葡萄糖传感器的影响，2）检查葡萄糖传感和信息素受体的直接物理相互作用，以及3）识别这些受体下游信号途径之间的相互作用。更广泛的影响：该项目在增强我们对酵母中营养感应和交配之间关系的理解的同时，将影响本科生和三个主要是本科机构的科学教师。 本科生将在一个密集的十周夏季项目中接受生化研究，并有机会在学年继续进行研究。 这些学生将参与其在同龄人社区中自己实验结果的设计，实施，解释和传播。 该项目为研究人员提供了将研究纳入教学的机会，从而增强了参与机构的数百名学生的科学教育。参与这项研究的学生将主要来自阿巴拉契亚农村地区通常服务不足的人口（包括代表性不足的少数民族）。