CAREER: Integrated Micro-Electro-Mechanical-System for Cellular Mechanotransduction Studies

职业：用于细胞力传导研究的集成微机电系统

• 批准号: 0954013
• 负责人: Yi Zhao
• 金额: $ 74.87万
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2017-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0954013&HistoricalAwards=false
• 关键词: CAREER; Integrated; Micro; Electro; Mechanical

Cellular mechanotransduction, the mechanism by which cells convert a mechanical stimulus into biochemical activities, widely affects a broad array of physiological processes. The mechanical stimulus is perceived and processed by cells and tissues through a highly complicated process regulated by a large number of biomolecules, subcellular components and extracellular structures, which eventually leads to appropriate biochemical responses. Understanding mechanotransduction requires engineering devices that can provide in situ assessment, in a controlled environment, of the cell response to systematically variable external mechanical stimuli mimicking the combinatorial mechanical loads (compressive/tensile) found in vivo.In this CAREER project, fully automated cellular mechanotransduction analysis will be streamlined through the development of an integrated micro-electro-mechanical-system (MEMS). The device will simplify the analysis of cells and tissues by minimizing external influence and will provide direct correlation between an applied mechanical stimulus and the activity of critical mechanosensitive biomolecules. The device will enable application of independently controllable combinational mechanical signals with preferred magnitudes, frequency, and duration to cells on loading sites. The use of a microfluidic network will allow parallel actuation of a large number of cells, and in situ intercellular and intracellular analysis. Use of the device will be simplified by developing instrumental and software interfaces. The efficacy of the device will be evaluated using skeletal myoblasts C2C12. By enabling quantitative study mechanotrandcution pathways, the device will help in the study of mechanisms that govern physiologic and pathologic conditions of mechanosensitive cells.The CAREER project will integrate the PI's research, education and outreach activities. The research outcome will be incorporated into courses and laboratory modules, benefitting students from both engineering fields and biology. The research discoveries will be disseminated through archival journals and conference presentations. Meanwhile, the research will provide a vehicle to deliver math, technological and engineering science in an exciting, practical, and interactive environment to minority and women students through the institutional Engineers in Motion program, and to K-12 students through collaboration with a local high school.The project outcome will also be available at the lab website http://www.bme.ohio-state.edu/yizhao/.

细胞机械力转导是细胞将机械刺激转化为生物化学活动的机制，广泛影响着广泛的生理过程。细胞和组织通过大量生物分子、亚细胞组分和细胞外结构调节的高度复杂的过程来感知和处理机械刺激，最终导致适当的生化反应。理解mechanodentransduction需要工程设备，可以提供原位评估，在受控环境中，细胞响应系统可变的外部机械刺激模仿组合的机械负荷（压缩/拉伸）在vivo.In这个CAREER项目中，全自动细胞mechanodentransduction分析将简化通过开发一个集成的微机电系统（MEMS）。该装置将通过最小化外部影响来简化细胞和组织的分析，并将提供所施加的机械刺激与关键机械敏感生物分子的活性之间的直接相关性。该装置将能够将具有优选幅度、频率和持续时间的独立可控的组合机械信号应用于加载位点上的细胞。微流体网络的使用将允许大量细胞的并行致动，以及原位细胞间和细胞内分析。通过开发仪器和软件接口，将简化设备的使用。将使用骨骼肌成肌细胞C2 C12评价器械的有效性。通过定量研究机械传导通路，该设备将有助于研究机械敏感细胞的生理和病理条件的机制。CAREER项目将整合PI的研究，教育和推广活动。研究成果将纳入课程和实验室模块，使工程领域和生物学领域的学生受益。研究发现将通过档案期刊和会议报告传播。同时，该研究将提供一种工具，通过机构工程师在运动计划中向少数民族和女性学生提供令人兴奋的，实用的和互动的环境中的数学，技术和工程科学，并通过与当地高中的合作向K-12学生提供。该项目成果也将在实验室网站http://www.bme.ohio-state.edu/yizhao/上提供。