Zytoskelettdynamik und Kraftgenerierung wandernder Leukozyten

迁移白细胞的细胞骨架动力学和力产生

• 批准号: 19738965
• 负责人: Professor Michael Sixt
• 金额: --
• 依托单位: Institute of Science and Technology Austria
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/19738965?language=en
• 关键词: Zytoskelettdynamik; und; Kraftgenerierung; wandernder; Leukozyten

Dendritic cells are potent primers of naïve T cells. Their function in vivo is highly dependent on their directed migration from the periphery where they take up antigens, towards the draining lymph node where they present the processed antigen to T cells. The main determinants that define the positioning and trafficking of leukocytes are chemokines that dictate the direction of migration and integrins that mediate the physical interaction with the cellular and extracellular environment. On leukocytes the functional state of integrins is controlled via chemokines. Using a genetic approach it will first be investigated in vivo which integrin families are responsible for which step during the migration of DC from the skin into the draining lymph node. Migratory or positioning defects will be studied in detail using video microscopy based in vitro assays. It will be defined at which level the chemotactic response is disturbed in the absence of integrins. Therefore, gradient sensing, cell polarization, adhesiveness, migration speed and directionality will be analyzed separately.

树突状细胞是幼稚T细胞的有效引物。它们在体内的功能高度依赖于它们从外周（在外周它们摄取抗原）向引流淋巴结（在引流淋巴结它们将加工的抗原呈递给T细胞）的定向迁移。定义白细胞定位和运输的主要决定因素是决定迁移方向的趋化因子和介导与细胞和细胞外环境的物理相互作用的整合素。在白细胞上，整合素的功能状态通过趋化因子控制。使用遗传学方法，将首先在体内研究哪些整合素家族负责DC从皮肤迁移到引流淋巴结期间的哪个步骤。迁移或定位缺陷将使用基于视频显微镜的体外试验进行详细研究。将定义在不存在整联蛋白的情况下，趋化反应在哪个水平受到干扰。因此，将分别分析梯度感应、细胞极化、细胞周期、迁移速度和方向性。