Adaptomics of neofunctionalization: analysis of GORDITA-like genes in Brassicaceae

该项目研究了一个单一的发育控制基因GORDITA （GOA）是如何通过基因复制和序列分化来促进芸苔科植物的适应性和适应性的。祖先的Bsister基因（abs型基因）参与胚珠发育，而衍生的goa型基因在果实发育中获得了新的表达域和功能。通过对来自不同十字花科植物的goa型和abs型基因的综合比较分析，我们将探讨导致现存goa型基因的谱系何时起源，以及这些基因在果实发育过程中获得其独特表达模式、结构域结构和功能的分子机制。为了更好地了解GOA的祖先是如何逃脱“大野耐一困境”的，我们将利用计算机方法分析油菜科植物系统发育过程中Bsister基因不同分支的分子进化。通过蛋白-蛋白相互作用研究，我们将研究蛋白质结构的变化如何改变GOA蛋白相互作用网络，并与ABS进行比较。为了评估GOA型基因的起源如何促进十字花科物种的适应，我们将使用转基因敲除植物确定温室条件下植物适应度的代理。并将为今后利用非转基因突变体测定自然生长条件下的植物适应性做准备工作。

The project investigates how the origin of a single developmental control gene, GORDITA (GOA), by gene duplication and sequence divergence contributed to plant fitness and adaptation in the Brassicaceae. While the ancestral Bsister genes (ABS-type genes) are involved in ovule development, the derived GOA-type genes acquired a new expression domain and function in fruit development. By comprehensive comparative analyses involving GOA-type and ABS-type genes from diverse Brassicales species we will investigate when the lineage that led to extant GOA-type genes originated, and by which molecular mechanisms these genes acquired their unique expression patterns, domain structures, and function during fruit development. To better understand how the ancestors of GOA escaped ‘Ohno’s dilemma’ we will analyze the molecular evolution in the different branches of Bsister genes during the phylogeny of Brassicales employing in silico methods. Using protein-protein interaction studies we will investigate how changes in the protein structure changed the protein interaction network of GOA in comparison of ABS. To assess how the origin of GOA-type genes contributed to the adaptation of Brassicaceae species we will determine proxies of plant fitness under greenhouse conditions using transgenic knock-out plants, and will do preparative work for future determination of plant fitness under natural growth conditions using non-transgenic mutants.