Adaptomics of neofunctionalization: analysis of GORDITA-like genes in Brassicaceae

该项目调查了一个单一的发育控制基因，拟南芥DITA（果阿）的起源，通过基因复制和序列分歧，有助于植物的健身和适应在菊科。虽然祖先Bsister基因（ABS型基因）参与胚珠发育，但衍生的GOA型基因获得了新的表达结构域和功能。通过全面的比较分析，涉及GOA型和ABS型基因从不同的拟南芥目物种，我们将调查时，导致现存的GOA型基因起源的血统，以及这些基因获得其独特的表达模式，结构域结构和功能的分子机制在果实发育过程中。为了更好地了解果阿的祖先如何逃脱“大野困境”，我们将分析在不同分支的Bsister基因的分子进化过程中，采用在硅片的方法？通过蛋白质-蛋白质相互作用研究，我们将研究与ABS相比，蛋白质结构的变化如何改变果阿的蛋白质相互作用网络。为了评估GOA型基因的起源是如何促进菊科物种的适应，我们将使用转基因敲除植物在温室条件下确定植物适合度的代理，并将为未来使用非转基因突变体在自然生长条件下确定植物适合度做准备工作。

The project investigates how the origin of a single developmental control gene, GORDITA (GOA), by gene duplication and sequence divergence contributed to plant fitness and adaptation in the Brassicaceae. While the ancestral Bsister genes (ABS-type genes) are involved in ovule development, the derived GOA-type genes acquired a new expression domain and function in fruit development. By comprehensive comparative analyses involving GOA-type and ABS-type genes from diverse Brassicales species we will investigate when the lineage that led to extant GOA-type genes originated, and by which molecular mechanisms these genes acquired their unique expression patterns, domain structures, and function during fruit development. To better understand how the ancestors of GOA escaped ‘Ohno’s dilemma’ we will analyze the molecular evolution in the different branches of Bsister genes during the phylogeny of Brassicales employing in silico methods. Using protein-protein interaction studies we will investigate how changes in the protein structure changed the protein interaction network of GOA in comparison of ABS. To assess how the origin of GOA-type genes contributed to the adaptation of Brassicaceae species we will determine proxies of plant fitness under greenhouse conditions using transgenic knock-out plants, and will do preparative work for future determination of plant fitness under natural growth conditions using non-transgenic mutants.