Functional diversification of class1 KNOX genes in basal eudicots

基础真双子叶植物 1 类 KNOX 基因的功能多样化

• 批准号: 19309724
• 负责人: Professorin Dr. Annette Becker
• 金额: --
• 依托单位: Arbeitsgruppe Spezielle Botanik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/19309724?language=en
• 关键词: Functional; diversification; class1; KNOX; genes

KNOX genes are a family of homeobox genes that are essential regulators of plant shoot development. KNOX genes are required for continued growth of the shoot apical meristem, and leaf formation requires KNOX genes to be downregulated. In several angiosperm lineages, an additional function of KNOX genes in the control of leaf dissection has evolved. However, evolutionary changes of KNOX gene action associated with modifications of leaf forms are not well understood. This project aims to characterize this small gene family and its role in leaf development in the basal eudicot plant Eschscholzia californica. Eschscholzia provides a unique system because of its dissected leaves, its phylogenetic position between core eudicots and basal angiosperms, and the availability of developmental genetic technologies. In addition, contrasting developmental patterns in related species invite within-family comparisons. To understand dissected leaf development, we will firstly characterize the class 1 KNOX genes and their expression patterns in Eschscholzia and the related genus Chelidonium. Secondly, phenotypic effects of modulated expression will be determined in Eschscholzia, using Agrobacterium-mediated transformation and Virus-induced gene silencing. To further assess differences in the action of KNOX genes between Eschscholzia and other species, and between dissected and simple leaves, the interaction with other genes and with phytohormones will be studied. Comparisons with other species will promote our understanding of how diversification of KNOX genes is implicated in morphological evolution.

KNOX基因是一个同源盒型基因家族，是植物茎部发育的重要调控因子。茎尖分生组织的持续生长需要KNOX基因，而叶片的形成则需要KNOX基因的下调。在一些被子植物谱系中，KNOX基因在控制叶片解剖中的附加功能已经进化。然而，KNOX基因作用的进化变化与叶形修饰的关系尚不清楚。本项目旨在描述这个小基因家族及其在基生植物加州紫苣苔叶片发育中的作用。由于其解剖的叶片，其系统发育位置介于核心被子植物和基生被子植物之间，以及发育遗传技术的可用性，因此提供了一个独特的系统。此外，相关物种的不同发育模式引起了科内比较。为了更好地理解解剖叶片的发育，我们首先分析了1类KNOX基因及其在紫堇和相关的Chelidonium属中的表达模式。其次，利用农杆菌介导的转化和病毒诱导的基因沉默，在埃氏菌中确定调节表达的表型效应。为了进一步研究KNOX基因在紫花苜蓿与其他物种、解剖叶与单叶之间的作用差异，将进一步研究其与其他基因以及与植物激素的相互作用。与其他物种的比较将促进我们对KNOX基因多样化在形态进化中的作用的理解。