Extracellar Hsp72 Is a DAMP Released by Stress

细胞外 Hsp72 是压力释放的 DAMP

• 批准号: 1022451
• 负责人: Monika Fleshner
• 金额: $ 43万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1022451&HistoricalAwards=false
• 关键词: Extracellar; Hsp72; DAMP; Released; Stress

The physiological stress response facilitates flight or fight responses and potentiates chances of survival by dilating pupils and increasing energy mobilization, heart rate, respiration, and blood flow to skeletal muscles. Current research indicates that potentiated innate immunity due to the release of danger-associated-molecular-patterns (DAMPs) should be added to this list. This project focuses on the role of the stress-inducible member of the 70-kDa Hsp (heat-shock) family of proteins Hsp72, as an important systemic DAMP. The discovery of Hsp72 as a DAMP would have implications for all organisms that generate adaptive acute stress responses. Using a systems biology approach, this project examines the nature of eHsp72 release and its function in host defense following bacterial challenge. The results of this work will enrich the interplay of research between the fields of stress physiology and immunology and could yield ways of capitalizing on the adaptive aspect of stress. In addition to specific scientific advancements, this research will sustain recruitment of undergraduate students from underrepresented minority groups in the biological sciences, with the goal of preparing them to enter graduate school. The PI has historically trained and mentored undergraduate students from underrepresented groups. Students involved in the project will be mentored and trained in research design, critical thinking, oral and written communication. Undergraduate students will present their work at scientific meetings and in peer-reviewed journals as co-authors. The project outcomes will be disseminated to the general public through television, radio, and mainstream periodicals.

生理应激反应促进飞行或战斗反应，并通过扩大瞳孔和增加能量动员、心率、呼吸和骨骼肌血流来增强生存机会。目前的研究表明，增强先天免疫，由于释放的DAMP相关的分子模式（DAMP）应添加到这个列表。该项目的重点是70 kDa热休克蛋白（热休克）家族的应激诱导成员Hsp72的作用，作为一个重要的系统DAMP。Hsp72作为DAMP的发现将对所有产生适应性急性应激反应的生物体产生影响。本项目采用系统生物学方法，研究了细菌攻击后eHsp72释放的性质及其在宿主防御中的功能。这项工作的结果将丰富应激生理学和免疫学领域之间的相互作用研究，并可能产生利用应激适应方面的方法。除了具体的科学进步外，这项研究将继续从生物科学中代表性不足的少数群体中招募本科生，目标是为他们进入研究生院做好准备。PI历来培训和指导来自代表性不足群体的本科生。参与该项目的学生将在研究设计，批判性思维，口头和书面沟通方面得到指导和培训。本科生将在科学会议上展示他们的工作，并作为合著者在同行评审的期刊上发表。项目成果将通过电视、广播和主流期刊向公众传播。