The Genetic Basis of Transgressive Variation in Rice

水稻海侵变异的遗传基础

• 批准号: 1026555
• 负责人: Susan McCouch
• 金额: $ 877.68万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2016-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1026555&HistoricalAwards=false
• 关键词: Genetic; Basis; Transgressive; Variation; Rice

PI: Susan R. McCouch (Cornell University)CoPI(s): Jason Mezey (Cornell University), Georgia C. Eizenga and Anna M. McClung (University of Arkansas/USDA-ARS Dale Bumpers National Rice Research Center)Key Collaborator(s): Stefan Einarson (Cornell University) and Lewis H. Ziska (USDA-ARS Beltsville Agricultural Research Center)This project will establish a joint forward-reverse genetics platform to facilitate the utilization of wild and exotic genetic materials in plant improvement and to accelerate the pace of discovery of genes and gene networks underlying transgressive variation in rice. Transgressive variation occurs in crosses between genetically divergent parents where offspring outperform the better parent. The nature of transgressive variation is that an individual locus' effect is dependent on other loci in the genetic background. Thus, the genetic dissection of transgressive variation necessitates modeling explicit gene interactions. Over the course of this project, models of genotype by genotype interaction(s) will be developed and validated, information that is key to manipulating non-additive genetic variation that can rapidly propel significant improvements in plant yield, adaptation and quality. In addition, this project will create a comprehensive genomics platform using wild and exotic rice germplasm to accelerate the pace of discovery of genes and gene networks underlying transgressive variation in rice for plant improvement. Specific objectives are to (1) re-sequence 30 diverse Oryza rufipogon accessions for the development of a 600K SNP chip for rice; (2) phenotype an expanded panel of wild and exotic rice germplasm for resistance to selected biotic and abiotic stresses; (3) create genetic materials for use in association mapping analyses and to rapidly translate discoveries into plant breeding applications; and, (4) develop integrated analysis techniques aimed at detecting non-additive genetic architecture associated with transgressive variation.This project aims to gain a deeper understanding of the breadth and depth of genotypic and phenotypic variation within the rice gene pool and to empower the rice community to efficiently translate this knowledge into practical applications in plant improvement. Educational outreach activities will provide diverse opportunities for interdisciplinary, hands-on learning for high school, undergraduate, graduate and postdoctoral fellows with emphasis on groups that are currently under-represented in science. The project will also continue and expand the 3-week Rice:Research to Production field-course in collaboration with the International Rice Research Institute (the Philippines). All of the data, germplasm and genomic resources developed on this project will be publicly available through project websites (www.ricediversity.org and www.ricesnp.org), the Gramene database (www.gramene.org), the International Rice Germplasm Center (IRGC), and the Genetic Stocks Center Oryza (GSOR) (www.gsor.gov).

PI：Susan R. McCouch（康奈尔大学）CoPI：Jason Mezey（康奈尔大学），格鲁吉亚C。Eizenga和安娜M. McClung（阿肯色州大学/USDA-ARS戴尔邦珀斯国家水稻研究中心）主要合作者：Stefan Einarson（康奈尔大学）和刘易斯H. Ziska（USDA-ARS贝尔茨维尔农业研究中心）该项目将建立一个联合正反向遗传学平台，以促进在植物改良中利用野生和外来遗传材料，并加快发现水稻超亲变异基因和基因网络的步伐。超越变异发生在遗传上不同的亲本之间的杂交中，其中后代表现优于更好的亲本。超亲变异的本质是一个位点的效应依赖于遗传背景中的其他位点。因此，对超亲变异的遗传解剖需要建立明确的基因相互作用模型。 在该项目的过程中，将开发和验证基因型与基因型相互作用的模型，这些信息是操纵非加性遗传变异的关键，可以迅速推动植物产量，适应性和质量的显着改善。 此外，该项目还将利用野生和外来水稻种质建立一个综合基因组学平台，以加快发现水稻超亲变异基因和基因网络的步伐，从而促进植物改良。 具体目标是（1）对30个不同的普通野生稻（Oryza rufipogon）种质进行重新测序，以开发用于水稻的600 K SNP芯片;（2）对扩大的一组野生和外来水稻种质进行表型鉴定，以确定其对选定的生物和非生物胁迫的抗性;（3）创建用于关联作图分析的遗传材料，并将发现快速转化为植物育种应用;以及（4）发展整合分析技术，以侦测与超亲变异相关的非加性遗传结构，此计划旨在更深入了解水稻基因库内基因型和表型变异的广度和深度，并使水稻社群能够有效地将这些知识转化为植物改良的实际应用。教育推广活动将为高中、本科、研究生和博士后研究员提供跨学科、动手学习的各种机会，重点是目前在科学领域代表性不足的群体。 该项目还将与国际水稻研究所（菲律宾）合作，继续并扩大为期3周的水稻：从研究到生产实地课程。 该项目开发的所有数据、种质和基因组资源将通过项目网站（www.ricediversity.org和www.ricesnp.org）、Gramene数据库（www.gramene.org）、国际水稻种质中心（IRGC）和水稻遗传资源中心（GSOR）（www.gsor.gov）公开提供。