Metabolic determinants in the interaction of biotrophic and hemibiotrophic fungi with cereals

生物营养型和半生物营养型真菌与谷物相互作用的代谢决定因素

• 批准号: 19985466
• 负责人: Professor Dr. Uwe Sonnewald
• 金额: --
• 依托单位: Lehrstuhl für Biochemie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/19985466?language=en
• 关键词: Metabolic; determinants; interaction; biotrophic; hemibiotrophic

Major objective of project B6 is to unravel molecular determinants underlying the regulation of plant primary metabolism by fungal effectors. In addition the relevance of selected metabolic processes during compatible plant/pathogen interaction will be investigated in conditional dsRNAi barley knock-down lines (B3) and maize transformants (A2, B3). To gain insight into metabolic and molecular changes induced during plant-microbe interaction, interactions of Ustilago maydis, Blumeria graminis, Erysiphe cichoracearum, Colletotrichum graminicola and Piriformospora indica with their respective hosts will be studied on the physiological, metabolic and transcriptional level. Data collected will be deposited and managed in a central database (central experiment coordinated by B7). Subsequent data mining will allow identifying key-metabolites and transcripts involved in compatible plant-microbe interactions. The significance of these findings will be tested in transgenic plants altered in expression of candidate genes (B3) and in subsequent infection studies. To gain insight into possible fungal determinants required for the successful invasion of plants, U. maydis mutants (A2), unable to successfully infect their host plants, will be included in these studies.

项目B6的主要目标是解开真菌效应器调节植物初级代谢的分子决定因素。此外，还将在条件dsRNAi大麦品系(B3)和玉米转化体(A2，B3)中研究亲和植物/病原菌相互作用期间选定的代谢过程的相关性。为了深入了解植物-微生物相互作用过程中引起的代谢和分子变化，将从生理、代谢和转录水平上研究玉米黑粉菌、小麦白粉菌、白蜡虫、禾谷炭疽菌和梨形孢霉与其各自寄主的相互作用。收集的数据将存储在中央数据库中并进行管理(中央实验由B7协调)。随后的数据挖掘将允许识别与植物-微生物相互作用相关的关键代谢物和转录本。这些发现的意义将在候选基因(B3)表达改变的转基因植物中得到检验，并在随后的感染研究中得到验证。为了深入了解成功入侵植物所需的可能真菌决定因素，无法成功感染寄主植物的玉米单胞菌突变体(A2)将被包括在这些研究中。

项目成果

The Ustilago maydis Nit2 Homolog Regulates Nitrogen Utilization and Is Required for Efficient Induction of Filamentous Growth

• DOI: 10.1128/ec.05191-11
• 发表时间: 2012-03-01
• 期刊: EUKARYOTIC CELL
• 作者: Horst, Robin J.;Zeh, Christine;Voll, Lars M.
• 通讯作者: Voll, Lars M.

Loss of cytosolic NADP-malic enzyme 2 in Arabidopsis thaliana is associated with enhanced susceptibility to Colletotrichum higginsianum.

• DOI: 10.1111/j.1469-8137.2012.04129.x
• 发表时间: 2012-07
• 期刊: The New phytologist
• 作者: L. Voll;Martina B. Zell;Timo Engelsdorf;Alexandra Saur;M. G. Wheeler;M. Drincovich;A. Weber;V. Maurino