Separating Science from Fiction: Introducing Liquid Chromatography in an Undergraduate Laboratory Curriculum

该项目的主要目标是为化学和生物化学学生提供在未来职业生涯中取得成功所必需的基本理解和实践经验。做到这一点的一个有效方法是及早并在整个化学和生物化学课程中引入现代化学仪器。这个项目开发了越来越复杂的实验室练习，这些练习利用了四个具有不同能力的液体色谱系统。层析是一种重要的仪器方法，在化学和生化学科中以各种形式使用，应用范围广泛，包括制药、食品、环境和生物医学分析和研究。练习从说明性的工作台练习开始，以动手体验结束。在各级课程的实验室中正在实施越来越复杂的练习，以便完成课程的学生对这项重要技术有很强的实用知识。这个项目的一个重要方面是在招生人数较多的实验室课程中实施液相色谱练习，比如大多数中等或大型大学教授的课程。这是通过使用说明性工作台练习的早期指导和通过应用多种最先进但负担得起的分离工具来实现的，这些工具允许快速分离。该项目还通过研讨会向当地高中科学教师介绍这项技术。此外，使用这种仪器的高中生将得到支持，参加当地和地区的科学博览会。

The major goal of the project is to provide chemistry and biochemistry students with the fundamental understanding and practical experience necessary to be successful in their future careers. One effective means for doing this is through introduction of modern chemical instrumentation early and throughout the chemistry and biochemistry curricula. This project develops laboratory exercises of increasing sophistication that utilize four liquid chromatography systems with varying capabilities. Chromatography is an important enabling instrumental method that is used in various forms across the chemical and biochemical disciplines for a wide variety of applications, including pharmaceutical, food, environmental and biomedical analyses and studies. The exercises begin with illustrative bench top exercises and culminate with hands-on experience. Exercises of increasing sophistication are being implemented in laboratories at all levels of the curriculum, such that students who complete the curriculum have a strong working knowledge of this important technology. An important aspect of this project is the implementation of liquid chromatography exercises in large-enrollment laboratory courses like those taught at most intermediate or large universities. This is accomplished through early instruction using illustrative bench top exercises and through application of multiple state-of-the-art yet affordable separations instruments that allow fast separations. The project is also introducing the technology to local high school science teachers through workshops. In addition, high school students that utilize the instrumentation are being supported to attend local and regional science fairs.