EAGER/Collaborative Research: Large Scale Microtubule-Based Nanomanufacturing of Single Kinesin Patterns with Ultrahigh Resolution

EAGER/合作研究：基于微管的超高分辨率单一驱动蛋白模式的大规模纳米制造

• 批准号: 1049150
• 负责人: Zoica Cerasela Dinu
• 金额: --
• 依托单位: West Virginia University Research Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1049150&HistoricalAwards=false
• 关键词: EAGER; Collaborative; Research; Large; Scale

The objective of this Early-Concept Grant for Exploratory Research (EAGER) project is to develop a novel concept for patterning single protein with ultrahigh resolution (below 10 nm). The approach is based on self-assembly and self-recognition and originates in the intracellular environment that is too crowded to allow diffusion to be an efficient mechanism for the movement of materials within the cytoplasm. Specifically, in situ polymerized microtubule (cytoskeletal filaments) affixed vertically to the tip of an atomic force microscope serves as track for purified kinesin molecular motors; under the chemical energy derived from adenosine triphosphate hydrolysis kinesin is deposited from the microtubule tip onto a glass surface situated in close proximity leading to nanoarrays of single protein. This EAGER-developed technology benefits society in areas as: drug delivery, screening, nanoelectronics, and nanosensors. Beyond recognizing the value of this technology through the proof of principle that biological molecules can be used for printing nanoarrays with ultrahigh resolution, this research also provides solutions to patterning individual nanomaterial (both organic and inorganic). The inherent interdisciplinary nature offers tremendous opportunities for enticing and integrating students with educational experience across diverse disciplines (two graduates will be employed by this program). The advances in the field of biomimetic-based nanomanufacturing will be incorporated in two courses Cellular machines at WVU and Processing of Biomaterials at RPI. Lastly, the PI will use Society for Biological Engineers at WVU to popularize bionanotechnology by generating inexpensive posters highlighting the advances and thus contributing to public education in nanotechnology and outreach to underrepresented populations, (i.e., women, rural communities).

这个探索性研究（EAGER）项目的早期概念资助的目标是开发一种超高分辨率（低于10纳米）单个蛋白质图案的新概念。该方法基于自组装和自我识别，起源于过于拥挤的细胞内环境，不允许扩散成为细胞质内物质运动的有效机制。具体来说，原位聚合微管（细胞骨架细丝）垂直贴在原子力显微镜的尖端，作为纯化的激酶分子马达的轨道；在三磷酸腺苷水解产生的化学能作用下，驱动蛋白从微管顶端沉积到靠近的玻璃表面，形成单个蛋白质的纳米阵列。这项由eager开发的技术在药物输送、筛选、纳米电子学和纳米传感器等领域对社会有益。除了通过证明生物分子可以用于超高分辨率纳米阵列打印的原理来认识到这项技术的价值之外，这项研究还为单个纳米材料（有机和无机）的图案化提供了解决方案。固有的跨学科性质为吸引和整合具有不同学科教育经验的学生提供了巨大的机会（该项目将雇用两名毕业生）。以仿生为基础的纳米制造领域的进展将被纳入WVU的细胞机器和RPI的生物材料加工两门课程中。最后，该项目将利用西弗吉尼亚大学的生物工程师协会来推广生物纳米技术，方法是制作廉价的海报，突出纳米技术的进步，从而促进纳米技术的公共教育，并向代表性不足的人群（即妇女、农村社区）推广纳米技术。