Nanomanufacturing of Single Molecule Patterns Using a Microtubule Pen

这项补助金为创造蘸笔的研究提供资金？生物笔？还是？biopen？能够写入单个生物分子的阵列，还是？生物墨水？具有高分辨率（即，低于10 nm）。从染料标记的生物墨水池中对biopen进行用户控制的充电是基于生物墨水在biopen上的分子识别和组装特性。biopen再充电过程允许控制在一个单独的书写会话中释放的生物墨水的量。通过调节生物墨水性质（即，墨水池中的浓度），将决定书写的速度、准确性和频率。将使用分子生物学和基于显微镜的分析来研究biopen充电并评估其写入性能。还将研究涉及允许使用同一biopen进行多个书写会话的条件分析的实验。这项研究的结果将导致大规模的进步？写作？有机和无机材料，如量子点和纳米管，具有更高的精度和更高的成本效益。所提出的方法的应用包括单个化合物的药物筛选和纳米电子系统的小型化和集成。这项工作的主要目标是确定允许biopen再充电的最佳条件，并评估其写入性能，以确保快速且高精度地形成生物墨水阵列。拟议的工作也将有助于一个更基本的理解biopens上的生物墨水的分子识别特性，并占一个动态的驱动力的观点，确定这种识别发生的热力学签名。

This grant provides funding for research in creating a dip quill ?biological pen? or ?biopen? capable of writing arrays of individual biomolecules or ?bioinks? with ultrahigh resolution (i.e., below 10 nm). User-controlled charging of the biopen from a dye-labeled bio-inkwell is based on the molecular recognition and assembly properties of the bioink onto the biopen. The biopen recharge process permits control over the amount of bioink to be released in one individual writing session. By adjusting the bioink properties (i.e., concentration in the inkwell), the speed, accuracy and frequency of writing will be determined. Molecular biology and microscopy-based assays will be used to investigate biopen recharging and assess its writing performance. Experiments involving the analysis of the conditions that allow multiple writing sessions using the same biopen will also be investigated. The results of this research will lead to advances in large-scale ?writing? of both organic and inorganic materials such as quantum dots and nanotubes with ultrahigh precision and at increased cost effectiveness. The applications of the proposed approach include drug screening of individual compounds and nanoelectronic systems miniaturization and integration. The primary goal of this work is to determine the optimum conditions that allow biopen recharging and assess its writing performance to ensure formation of bioink arrays in rapid time and with high accuracy. The proposed work will also contribute to a more fundamental understanding of the molecular recognition properties of the bioinks on biopens and account for a dynamic view of the driving forces determining the thermodynamic signatures for such recognition to occur.