Nanomanufacturing of Single Molecule Patterns Using a Microtubule Pen
使用微管笔进行单分子图案的纳米制造
基本信息
- 批准号:1300757
- 负责人:
- 金额:$ 30.13万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2013
- 资助国家:美国
- 起止时间:2013-10-01 至 2018-10-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This grant provides funding for research in creating a dip quill ?biological pen? or ?biopen? capable of writing arrays of individual biomolecules or ?bioinks? with ultrahigh resolution (i.e., below 10 nm). User-controlled charging of the biopen from a dye-labeled bio-inkwell is based on the molecular recognition and assembly properties of the bioink onto the biopen. The biopen recharge process permits control over the amount of bioink to be released in one individual writing session. By adjusting the bioink properties (i.e., concentration in the inkwell), the speed, accuracy and frequency of writing will be determined. Molecular biology and microscopy-based assays will be used to investigate biopen recharging and assess its writing performance. Experiments involving the analysis of the conditions that allow multiple writing sessions using the same biopen will also be investigated. The results of this research will lead to advances in large-scale ?writing? of both organic and inorganic materials such as quantum dots and nanotubes with ultrahigh precision and at increased cost effectiveness. The applications of the proposed approach include drug screening of individual compounds and nanoelectronic systems miniaturization and integration. The primary goal of this work is to determine the optimum conditions that allow biopen recharging and assess its writing performance to ensure formation of bioink arrays in rapid time and with high accuracy. The proposed work will also contribute to a more fundamental understanding of the molecular recognition properties of the bioinks on biopens and account for a dynamic view of the driving forces determining the thermodynamic signatures for such recognition to occur.
这项拨款为制作浸笔的研究提供资金。生物的钢笔吗?或biopen ?能够写入单个生物分子阵列或“生物墨水”?具有超高分辨率(即低于10纳米)。用户控制的生物墨水从染料标记的生物墨水瓶充电是基于分子识别和生物墨水在生物墨水上的组装特性。生物开放充电过程允许控制在一个单独的写作会话中释放的生物链接的数量。通过调整生物墨水的特性(即墨水瓶中的浓度),可以确定书写的速度、准确性和频率。分子生物学和基于显微镜的分析将用于研究生物开放充电和评估其书写性能。实验包括分析允许使用同一生物开放的多个写作会话的条件,也将被调查。这项研究的结果将导致大规模写作的进步。有机和无机材料,如量子点和纳米管,具有超高精度和更高的成本效益。该方法的应用包括单个化合物的药物筛选和纳米电子系统的小型化和集成化。这项工作的主要目标是确定允许生物开放充电的最佳条件,并评估其写入性能,以确保在快速和高精度的情况下形成生物链接阵列。所提出的工作也将有助于更基本地理解双开口上生物墨水的分子识别特性,并从动态角度解释决定这种识别发生的热力学特征的驱动力。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Zoica Cerasela Dinu其他文献
Zoica Cerasela Dinu的其他文献
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{{ truncateString('Zoica Cerasela Dinu', 18)}}的其他基金
CAREER: Tailored metal-oxide-based heterogeneous nanointerfaces for robust electro-catalyst formation
职业:定制基于金属氧化物的异质纳米界面,用于稳定的电催化剂形成
- 批准号:
1454230 - 财政年份:2015
- 资助金额:
$ 30.13万 - 项目类别:
Standard Grant
EAGER/Collaborative Research: Large Scale Microtubule-Based Nanomanufacturing of Single Kinesin Patterns with Ultrahigh Resolution
EAGER/合作研究:基于微管的超高分辨率单一驱动蛋白模式的大规模纳米制造
- 批准号:
1049150 - 财政年份:2010
- 资助金额:
$ 30.13万 - 项目类别:
Standard Grant
User-controlled Biocatalysis Induces Self-decontamination of Enzyme-based Composites Challenged with Simulant of B. anthracis
用户控制的生物催化诱导受到炭疽芽孢杆菌模拟物挑战的酶基复合材料的自净化
- 批准号:
1033266 - 财政年份:2010
- 资助金额:
$ 30.13万 - 项目类别:
Standard Grant
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