Nanomanufacturing of Single Molecule Patterns Using a Microtubule Pen

这笔赠款为创造一种浸泡羽毛笔？生物笔？的研究提供资金。或者？生物笔？能够写出单个生物分子或生物墨水的阵列？具有超高分辨率(即低于10 nm)。从染料标记的生物墨水井对双开孔进行用户控制的充电是基于生物墨水在双开孔上的分子识别和组装特性。双开充电过程允许控制在一个单独的书写过程中释放的生物墨水的量。通过调整生物墨水的性质(即墨水池中的浓度)，将确定书写的速度、精度和频率。将使用分子生物学和基于显微镜的分析来研究双开充电并评估其书写性能。还将调查涉及允许使用同一双开本进行多个写作会话的条件分析的实验。这项研究的结果将促进大规模写作的进步。有机和无机材料，如量子点和纳米管，具有超高精度和更高的成本效益。该方法的应用包括单个化合物的药物筛选和纳米电子系统的小型化和集成化。这项工作的主要目标是确定允许双开充电的最佳条件并评估其写入性能，以确保快速、高精度地形成生物墨水阵列。拟议的工作还将有助于更基本地了解生物墨水在两个开口上的分子识别特性，并解释决定这种识别发生的热力学特征的驱动力的动态观点。

This grant provides funding for research in creating a dip quill ?biological pen? or ?biopen? capable of writing arrays of individual biomolecules or ?bioinks? with ultrahigh resolution (i.e., below 10 nm). User-controlled charging of the biopen from a dye-labeled bio-inkwell is based on the molecular recognition and assembly properties of the bioink onto the biopen. The biopen recharge process permits control over the amount of bioink to be released in one individual writing session. By adjusting the bioink properties (i.e., concentration in the inkwell), the speed, accuracy and frequency of writing will be determined. Molecular biology and microscopy-based assays will be used to investigate biopen recharging and assess its writing performance. Experiments involving the analysis of the conditions that allow multiple writing sessions using the same biopen will also be investigated. The results of this research will lead to advances in large-scale ?writing? of both organic and inorganic materials such as quantum dots and nanotubes with ultrahigh precision and at increased cost effectiveness. The applications of the proposed approach include drug screening of individual compounds and nanoelectronic systems miniaturization and integration. The primary goal of this work is to determine the optimum conditions that allow biopen recharging and assess its writing performance to ensure formation of bioink arrays in rapid time and with high accuracy. The proposed work will also contribute to a more fundamental understanding of the molecular recognition properties of the bioinks on biopens and account for a dynamic view of the driving forces determining the thermodynamic signatures for such recognition to occur.