IDBR: Solid State Patch-Clamping with Stealth Probes

IDBR：采用隐形探针的固态膜片钳

• 批准号: 1063397
• 负责人: Nicholas Melosh
• 金额: $ 38.3万
• 依托单位: Stanford University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1063397&HistoricalAwards=false
• 关键词: IDBR; Solid; State; Patch; Clamping

IDBR: Solid State Patch-Clamping with Stealth ProbesElectrical measurements of cell activity play a critical role in understanding neural communication and testing for adverse reactions to pharmaceutical therapies. However, current measurement techniques either cause rapid cell death or provide low-quality data, severely limiting monitoring and understanding of these activities. There is thus a compelling need for a new instrument that provides long-duration, high-quality electrical cell measurements that is easy to use and can measure a number of cells at the same time. The key obstacle is creating an intimate junction between the cell membrane and a cell-penetrating electrode. This research program explores a unique approach to this problem by creating metallic electrodes that mimic the structure and functionality of biological transmembrane proteins. These electrodes are designed to fuse into the lipid membrane, enabling direct electrical access into the cell without leakage. Electrode structure, surface modification and size will be optimized to provide the best electrical junctions and cell longevity. The final architecture will be developed into a simple to use, 96-electrode platform for low-noise, long-term electrical cell measurements. The broader impact of this work is greatly enhancing the number, quality, and duration of electrophysiological recording and stimulation, as well as training undergraduate students, graduate students, and middle school teachers in interdisciplinary scientific research. The final platform will dramatically impact studies of neural networks, neuron physiology, and drug screening, where slow testing rates and poor cell viability limits the number and types of experiments that can be performed. With this device, interconnected networks of up to 96 neurons could be stimulated and recorded simultaneously, allowing an unprecedented view of the evolution of sub-threshold voltage signaling in neural networks. These platforms will also play a crucial role in faster validation and screening for potential side effects of drug candidates, enhancing public health and safety.

IDBR：采用隐形Probe的固态膜片钳技术细胞活动的电学测量在了解神经通讯和测试药物治疗的不良反应方面发挥着关键作用。然而，目前的测量技术要么导致细胞迅速死亡，要么提供低质量的数据，严重限制了对这些活动的监测和了解。因此，迫切需要一种新的仪器，能够提供长时间、高质量的电池测量，而且易于使用，并且可以同时测量多个电池。关键的障碍是在细胞膜和穿透细胞的电极之间建立一个紧密的连接。这项研究计划探索了一种独特的方法来解决这个问题，通过创造金属电极，模拟生物跨膜蛋白的结构和功能。这些电极被设计成融合到脂膜中，从而能够直接电进入细胞而不会泄漏。电极结构、表面修饰和尺寸将得到优化，以提供最佳的电连接和电池寿命。最终的架构将被开发成一个简单易用的96电极平台，用于低噪音、长期的电池组测量。这项工作的更广泛影响是大大提高了电生理记录和刺激的数量、质量和持续时间，以及培训本科生、研究生和中学教师进行跨学科科学研究。最终的平台将极大地影响神经网络、神经元生理学和药物筛选的研究，在这些领域，缓慢的测试速度和糟糕的细胞活性限制了可以进行的实验的数量和类型。使用这种设备，可以同时刺激和记录多达96个神经元的相互连接的网络，从而以前所未有的方式观察神经网络中亚阈值电压信号的演变。这些平台还将在更快地验证和筛选候选药物的潜在副作用、加强公共健康和安全方面发挥关键作用。