Conference: Keystone Symposia meeting on "AAA+ and Related ATP-Driven Protein Machines" being held at the Granlibakken Resort, Tahoe City, CA

知识价值。本次“AAA+和相关atp驱动的蛋白质机器：结构、功能和机制”研讨会的目标是促进新概念和新方法的发展，以深入理解一系列复杂细胞过程背后的分子机制。会议的重点是需要由AAA+（与多种细胞活动相关的atp酶）分子机器家族提供机械工作的过程。这些蛋白质组合属于一类特殊的酶，它们参与了关键的生物过程，包括RNA从DNA的转录，细胞分裂前DNA的复制，蛋白质的生物合成和周转，与细胞膜从细胞的一部分到另一部分的运输相关的高度调节过程，以及动态蛋白质支架或“细胞骨架”的重塑，这赋予了细胞形状和流动性。这次会议将汇集具有非常不同背景和观点的研究人员：将生物物理方法应用于复杂系统的物理科学家，以及寻求对基本细胞过程进行深入机制理解的生物学家。本次会议旨在通过弥合方法和学科界限来促进创新。事实上，会议计划将汇集来自不同领域的演讲者，他们通常很少有机会互动，但已经阐明了不同AAA+蛋白质的关键方面。演讲者还将包括那些在理解不属于AAA+家族但在功能或结构上相关的蛋白质方面取得突破的人。这些观点超越了分子尺度，从细胞到原子水平，以及方法，从细胞生物学和遗传学到单分子生物物理学。这种结合可能会导致深入了解这些蛋白质的共同机制，并产生新的实验方法和发现。更广泛的影响。本次会议是围绕激发AAA+蛋白研究的新思路和新方法的中心目标构思和组织的。为了达到这些目标，演讲者被选中，他们是许多不同生物学领域的先驱，或者在不同分析水平（例如，结构，化学，细胞）上取得了最近的概念或实验突破。本次会议将通过促进网络和合作来加强基础设施。关于培训生和新调查员的教育效益，会议将实现以下目标：1)使这些人熟悉最新的信息；2)鼓励批判性的科学分析；3)促进他们的职业发展，有机会参与前沿科学的讨论，并与可能有助于他们的科学和专业发展的知名科学家建立联系。

Intellectual merit. The goal of this Keystone Symposium on "AAA+ and Related ATP-Driven Protein Machines: Structure, Function and Mechanism" is to stimulate the development of novel concepts and approaches directed at a deep understanding of the molecular mechanisms underlying a set of complex cellular processes. The conference focuses on processes that require mechanical work powered by the AAA+ (ATPases Associated with diverse cellular Activities) family of molecular machines. These protein assemblies belong to a special class of enzymes that are involved in key biological processes including transcription of RNA from DNA, the replication of DNA preceding cell division, the biosynthesis and turnover of proteins, the highly regulated processes associated with trafficking of membranes from one part of the cell to another, and remodeling of the dynamic protein scaffolding or "cytoskeleton," which gives the cell its shape and mobility. This meeting will bring together researchers with very diverse backgrounds and perspectives: physical scientists, who are applying biophysical approaches to complex systems, and biologists, who seek deep mechanistic understanding of fundamental cellular processes. This meeting aims to foster innovation by bridging methodological and disciplinary boundaries. Indeed, the meeting program will bring together speakers from diverse fields who typically have had few opportunities to interact but have elucidated critical aspects of different AAA+ proteins. Speakers will also include those who have made breakthroughs in the understanding of proteins that do not belong to the AAA+ family but are either functionally or structurally related. The perspectives transcend both molecular scales, from the cellular to the atomic level, as well as methodologies, from cell biology and genetics to single-molecule biophysics. This combination will likely lead to insights into the common mechanism of these proteins and to novel experimental approaches and discoveries. Broader impacts. This conference was conceived and organized around the central goal to stimulate new ideas and novel approaches in AAA+ protein research. To attain these goals, speakers were selected who have been pioneers in many different biological areas or who have achieved recent conceptual or experimental breakthroughs at different levels of analysis (e.g., structural, chemical, cellular). This meeting will enhance infrastructure by fostering networking and collaborations. With regard to the educational benefits to trainees and new invesitigators in attendance, the meeting will achieve the following: 1) acquaint these individuals with state-of-the-art information; 2) encourage critical scientific analysis; and 3) promote their career development with opportunities to participate in the discussion of cutting-edge science and to network with established scientists who might contribute to their scientific and professional development.