Genetic and Genomic Approaches to Understanding the Role of Auxin in Shoot Development

了解生长素在芽发育中的作用的遗传和基因组方法

• 批准号: 1114484
• 负责人: Paula McSteen
• 金额: $ 346.7万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2016-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1114484&HistoricalAwards=false
• 关键词: Genetic; Genomic; Approaches; Understanding; Role

PI: Paula McSteen (University of Missouri - Columbia)CoPIs: Simon Malcomber (California State University - Long Beach), Andrea Gallavotti and Yunde Zhao (University of California - San Diego)Senior Personnel: Naomi Altman and Reka Albert (The Pennsylvania State University)Collaborator: Robert Schmidt (University of California - San Diego)The hormone auxin regulates almost every aspect of plant growth and development, including leaf and flower formation, and stem and flower cluster (inflorescence) branching. The long-term goal of this project is to understand how shoot (the above ground portion of a plant) growth is regulated through pathways of auxin biosynthesis, transport and signal transduction, and the extent to which these mechanisms and interactions are conserved among the approximately 250,000 described species of flowering plants. Genes that function in auxin mediated shoot development will be identified and characterized using a combination of high throughput forward genetics in maize and comparative expression profiling in both maize and Arabidopsis. Reverse genetic analyses will be used to uncover the function of genes in these pathways that may not be apparent from forward genetic approaches alone. Gene function will be analyzed using transgenic reporter lines coupled with phenotypic, expression and genetic analyses of characterized mutants to assemble a network of genetic interactions. A gene regulatory network model will be built to integrate the data from expression profiling together with genetic and biochemical interaction studies. Phylogenetic characterization of identified gene families using available genomic data and genes isolated during the course of the research will provide a framework and series of testable hypotheses concerning the conservation and diversification of auxin mechanisms in all flowering plants. The findings from this project will lead to a better understanding of how auxin controls shoot growth and development in agronomically and economically important crops.Through a series of training and outreach programs this project will provide opportunities for high school students, undergraduates, graduate students, and postdoctoral researchers to learn and apply a broad range of genetic, developmental, evolutionary, genomic, statistical, mathematical and bioinformatic tools. Annual summer workshops and exchange programs will augment inter-disciplinary plant science training and dialog across fields. Results from the project will be incorporated into the Botanical Society of America's Planting Science program (http://www.plantingscience.org/) to create online open-ended, inquiry-based educational materials focusing on the role of auxin and the interdisciplinary nature of plant biology for high school students across the nation. Materials from this project will provide new resources and tools to investigate the role played by auxin in plant growth and development across flowering plants, and new information and strategies for improving crop yield in diverse crop species. Project data will be made available through the project website (http://www.auxinevodevo.org). In addition, mutant stocks will be available from the Maize Coop Stock Center (http://maizecoop.cropsci.uiuc.edu/), phenotypic and genetic data including map locations will be deposited at MaizeGDB (http://www.maizegdb.org/), expression profiling data will be deposited at the Plant Expression Database (http://www.plexdb.org/), phylogenetic trees will be deposited at TREEBASE (http://www.treebase.org) and sequences will be deposited in Genbank(http://www.ncbi.nlm.nih.gov/) and Plant GDB (http://www.plantgdb.org).

PI：Paula McSteen（密苏里大学 - 哥伦比亚大学）COPIS：Simon Malcomber（加利福尼亚州立大学 - 长滩），Andrea Gallavotti和Yunde Zhao（加利福尼亚大学 - 圣地亚哥大学 - 圣地亚哥大学）高级人员：Naomi Altman和Reka Albert（宾夕法尼亚州立大学）植物生长和发育的几乎每个方面，包括叶子和花朵形成，以及茎和花簇（花序）分支。该项目的长期目标是通过生长素生物合成，运输和信号转导的途径来了解芽（植物的上层部分）如何调节生长，以及这些机制和相互作用在大约250,000个所描述的开花植物物种中保存的程度。在玉米和玉米和拟南芥中，将在生长素介导的芽发育中发挥作用的基因，并通过高吞吐量前向遗传学和比较表达分析来鉴定和表征。反向遗传分析将用于揭示基因在这些途径中的功能，而这些途径可能仅从正向遗传方法中就可以看出。将使用转基因报道系与表型，表达和表征突变体的遗传分析一起分析基因功能，以组装遗传相互作用网络。将建立一个基因调节网络模型，以将表达分析与遗传和生化相互作用研究结合在一起。使用可用的基因组数据和研究过程中分离的基因的系统发育表征将提供有关所有开花植物中生长素机制的保护和多样化的框架和一系列可检验的假设。该项目的发现将使更好地理解生长素如何控制农业和经济上重要的农作物中的生长和发展。通过一系列培训和外展计划，该项目将为高中生，本科生，研究生，研究生以及博士后研究人员学习和应用遗传学，发展，发展，进化，基因组，基因组，统计学上的数学，数学和bio nigational，Mathicational，Mathemication，Mathemication和Bio。年度夏季研讨会和交流计划将增加跨学科的植物科学培训和跨领域的对话。该项目的结果将纳入美国植物学学会的种植科学计划（http://www.plantingscience.org/），以创建基于开放式的，基于询问的在线开放式教育材料，重点介绍了生长素和整个国家的高中生植物生物学的跨学科性质的作用。该项目的材料将提供新的资源和工具，以调查生长素在开花植物的植物生长和开发中所起的作用，以及提高各种农作物物种作物产量的新信息和策略。项目数据将通过项目网站（http://www.auxinevodevo.org）提供。 此外，将从玉米合作库存中心（http://maizecoop.cropsci.uiuc.edu/）提供突变库存，包括地图位置在内的表型和遗传数据将存放在MaizeGDB（http://wwwwwwwww.maizegdb.org/），表达数据的表达数据将沉积在玉米中的位置和遗传数据。 （http://www.plexdb.org/），系统发育树将沉积在Treebase（http://www.treebase.org）中，序列将沉积在GenBank（http://wwwwwww.ncbi.nlm.nih.gov/）和gdb（http://wwwwwww.nlm.nih.gov/）中。 （http://www.plantgdb.org）。