RESEARCH-PGR: Genomic and Synthetic Approaches Linking Auxin Signaling to Functional Domains in Maize

研究-PGR：将生长素信号传导与玉米功能域联系起来的基因组和合成方法

• 批准号: 1546873
• 负责人: Paula McSteen
• 金额: $ 364.31万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-15 至 2023-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1546873&HistoricalAwards=false
• 关键词: RESEARCH; PGR; Genomic; Synthetic; Approaches

Imagine any characteristic of plants - from development of shoots and roots to responses to the environment and pests - and it is almost certainly shaped by the plant growth hormone, auxin. Plant cells have the ability to detect and respond to auxin. Thus, characterizing the network of molecular components that involved in detection and response will improve our understanding of the multiple auxin effects. The primary goal of this project is to identify the auxin-regulated molecular networks in corn, an economically important crop in USA. Characterizing the roles these networks play in the growth and development in the tassel and ear of corn will help devise strategies to improve yields. A team of scientists from four different American universities will carry out this work using the most modern biological techniques. This work will fundamentally change the landscape of developmental biology in corn, and will significantly accelerate the transfer of knowledge from the lab to the field. Results from this work will made publically available. The research team is strongly committed to mentoring a diverse group of future scientists and to increasing broad scientific literacy. This project will organize workshops aimed at expanding the professional skillsets of undergraduate, graduate and postdoctoral trainees. Scientists from the four institutions will collaborate to develop training modules that will involve hands-on experimentation. These modules will specifically target audiences from K-12 schools, colleges, and the public at large.This project addresses a cornerstone of developmental biology: how auxin action is specified by context-specific deployment of signaling components, and in particular, how auxin controls reproductive organogenesis. Team members have amassed a wealth of genetic, genomic and molecular resources in maize to answer the question of how axillary meristems are initiated to give rise to the reproductive structures in the tassel and ear. Prior work has shown that components of the auxin signaling machinery are expressed in three functional domains within the inflorescence: the suppressed bract leaf, the boundary domain, and the axillary meristem. Connecting auxin signaling modules with tissue-level events will require a detailed understanding of which interactions occur in specific tissues and at specific times. This challenge will be met with a highly integrated approach incorporating genomic and synthetic biology tools with sophisticated informatics and data visualization. Specifically, this project will: 1) define and characterize the auxin signaling modules (receptors, repressors, transcription factors) operating within functional domains of the inflorescence; and 2) construct gene regulatory networks incorporating co-expressed genes and targets of the auxin signaling modules.

想象一下植物的任何特征 - 从芽和根的发展到对环境和害虫的反应 - 几乎可以肯定地由植物生长激素生长素塑造。植物细胞具有检测和反应生长素的能力。因此，表征与检测和响应有关的分子成分网络将提高我们对多种生长素效应的理解。该项目的主要目标是确定玉米中生长素调节的分子网络，玉米是美国经济上重要的农作物。表征这些网络在流苏和玉米耳朵的增长和发展中所起的作用将有助于制定提高产量的策略。来自四个不同大学的科学家团队将使用最现代的生物学技术进行这项工作。这项工作将从根本上改变玉米中发育生物学的景观，并会大大加速知识从实验室到现场的转移。这项工作的结果将公开可用。研究团队强烈致力于指导一群未来的科学家组，并提高广泛的科学素养。该项目将组织旨在扩大本科，研究生和博士后学员的专业技能的研讨会。来自四个机构的科学家将合作开发涉及动手实验的培训模块。这些模块将专门针对K-12学校，大学和广大公众的受众。该项目介绍了发育生物学的基石：生长素作用是如何通过特定于上下文的信号传导部署来指定的，尤其是生长素如何控制生殖器机。团队成员在玉米中积累了大量的遗传，基因组和分子资源，以回答如何发起腋窝分生组织的问题，以引起流苏和耳朵中的生殖结构。先前的工作表明，生长素信号机械的组件在花序内的三个功能域中表达：抑制的片叶，边界域和腋生分生组织。将生长素信号传导模块与组织级事件相连，将需要详细了解在特定组织中和特定时间发生哪些相互作用。将采用高度集成的方法来应对这一挑战，该方法结合了基因组和合成生物学工具，并具有复杂的信息学和数据可视化。具体而言，该项目将：1）定义和表征在花序功能域内运行的生长素信号模块（受体，阻遏物，转录因子）； 2）构建了包含生长素信号模块共表达基因和靶标的基因调节网络。