Mechanisms of Plant Steroid Hormone Regulated Gene Expression in Arabidopsis

拟南芥植物类固醇激素调控基因表达的机制

• 批准号: 1122166
• 负责人: Yanhai Yin
• 金额: $ 13.5万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1122166&HistoricalAwards=false
• 关键词: Mechanisms; Plant; Steroid; Hormone; Regulated

Plant steroid hormones, Brassinosteroids (BRs), play important roles in plant growth, development and response to environmental stresses. BRs act through several signaling components to regulate BES1/BZR1 and other families of transcription factors (TFs), which in turn control the expression of about 4000 genes required for various BR responses. How BR-regulated TFs control the expression of the large number of target genes and various BR responses is not well defined and will be the main focus of the proposal. The functions and mechanisms of several BR-regulated transcription factors (proteins responsible for activating or inactivating genes) will be studied by reverse genetic and genomic approaches and their regulation by BR signaling will be investigated by biochemical experiments. The results will reveal how each of the BR-regulated TFs controls a subset of BR target genes and a portion of BR responses. The studies will also provide important insight into how BR signal is integrated into the complex transcriptional network to control various BR-regulated processes. The knowledge obtained can be used to design strategies to improve the crop yield and tolerance to environmental stresses. Both mutants and transgenic lines generated from the project will be made available through the Arabidopsis Information Resource. In addition, the large-scale gene expression data will be deposited into NASC Arrays or GEO Datasets for wide dissemination. The proposed research will generate new knowledge that is essential for agriculture and bioenergy, especially under the constant environmental changes. In addition, a graduate student, research assistant and high school teacher will be trained to carry out the proposed experiments as a coordinated and cohesive team. The high school biology teacher will also be trained to bring some of the BR mutants to high school biology classes. The proposal, therefore, has a great potential in training future scientists and educators.

植物类固醇激素油菜素类固醇(BRs)在植物的生长发育和对环境胁迫的响应中起着重要的作用。BRs通过几个信号元件来调节BES1/BZR1和其他转录因子家族，这些转录因子又控制着大约4000个基因的表达，这些基因是各种BR反应所必需的。BR调控的转录因子如何控制大量目的基因的表达和各种BR反应还没有很好的定义，这将是本提案的主要焦点。几种BR调节转录因子(负责激活或失活基因的蛋白质)的功能和机制将通过反向遗传学和基因组学方法进行研究，它们对BR信号的调节将通过生物化学实验进行研究。结果将揭示每个受BR调节的转录因子如何控制BR靶基因的子集和BR反应的一部分。这些研究还将为BR信号如何整合到复杂的转录网络中以控制BR调节的各种过程提供重要的见解。所获得的知识可用于设计策略，以提高作物产量和对环境胁迫的耐受性。该项目产生的突变体和转基因品系都将通过拟南芥信息资源提供。此外，大规模的基因表达数据将被存储到NASC阵列或GEO数据集中进行广泛传播。拟议的研究将产生对农业和生物能源至关重要的新知识，特别是在环境不断变化的情况下。此外，一名研究生、研究助理和高中教师将接受培训，以作为一个协调和有凝聚力的团队来实施拟议的实验。这位高中生物老师还将接受培训，将一些BR突变体带到高中生物课上。因此，这项提议在培养未来的科学家和教育工作者方面具有巨大的潜力。