Genetic Biochemical Studies of Plant Steroid Signaling

植物类固醇信号传导的遗传生化研究

• 批准号: 10481527
• 负责人: ZHIYONG WANG
• 金额: $ 6.39万
• 依托单位: CARNEGIE INSTITUTION OF WASHINGTON, D.C.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10481527
• 关键词: Animals; Arabidopsis; Automobile Driving; Binding; Biochemical; Biological Models; Carbon; Cell division; Cells; Development; Developmental Process; Environmental Risk Factor; Equilibrium; Family; Genetic; Genetic Transcription; Genomics; Goals; Growth; Health; Hormones; Human; Investigation; Link; Membrane; Metabolic; Metabolism; Molecular; Nutrient; Personal Satisfaction; Phosphoric Monoester Hydrolases; Phosphotransferases; Physiological Processes; Plants; Post-Translational Protein Processing; Process; Proteomics; Research; Signal Pathway; Signal Transduction; Steroids; System; Transcriptional Activation; cell growth regulation; food security; improved; information processing; plant growth/development; programs; protein protein interaction; receptor; steroid hormone; trafficking; transcription factor

The long-term goal of this project is to understand the molecular networks through which hormones, environmental factors, and nutrients together control plant growth and development. Plants have evolved robust cellular signaling systems to regulate growth and metabolism according to internal status and environmental conditions. Dissecting these plant regulatory systems is important not only for food security and environmental sustainability, but also for understanding cellular regulation in general, as many mechanisms are highly conserved in animals and plants. This research program focuses on the cellular signaling and regulatory network impinged upon by the steroid hormone brassinosteroid (BR), a major growth-promoting hormone that impact a wide range of developmental and physiological processes. Using a combination of genetic, genomic, biochemical, and proteomic approaches in the Arabidopsis model system, we have elucidated molecular mechanisms by which BR binding to receptor kinase BRI1 leads to activation of transcription factor BZR1 through the evolutionarily conserved BSU1 family of phosphatases and GSK3-like kinases. Furthermore, we have revealed at molecular level how the BR signaling pathway is integrated with many other signaling pathways into regulatory networks that control gene transcription program driving cell elongation and various specific developmental programs. Our ongoing research has uncovered many posttranslational mechanisms by which BR signaling directly modulates cellular reorganization and metabolic programs through direct protein- protein interactions and posttranslational modifications. We proposed to focus on these mechanisms that link signaling pathways with major cellular activities such as membrane trafficking, cell division, and carbon metabolism. In addition, we will investigate how BR signaling cross talks with other signaling pathways to balance metabolism with growth demand and to program cellular differentiation in plant development. The research outlined in this proposal will continue to significantly advance our understanding of the molecular mechanisms of cellular signal integration and information processing.

这个项目的长期目标是了解分子网络，通过它 激素、环境因素和营养物质共同控制植物的生长和发育。 植物进化出强大的细胞信号系统来调节生长和新陈代谢 根据内部状况和环境条件。剖析这些植物监管 系统不仅对粮食安全和环境可持续性很重要，而且对 了解一般的细胞调节，因为许多机制在动物中高度保守 和植物。这项研究的重点是细胞信号和调节网络。 受到类固醇激素油菜素类固醇(BR)的冲击，BR是一种主要的促生长激素 这影响了广泛的发育和生理过程。使用以下组合 在拟南芥模型系统中的遗传、基因组、生化和蛋白质组方法，我们 阐明了BR与受体激酶BRI1结合导致 转录因子BZR1通过进化保守的BSU1家族激活 磷酸酶和GSK3样激酶。此外，我们还在分子水平上揭示了 BR信号通路与许多其他信号通路整合成调控网络 它控制着驱动细胞伸长和各种特定发育基因转录程序 程序。我们正在进行的研究发现了许多翻译后机制，BR通过这些机制 信号通过直接蛋白质直接调节细胞重组和代谢程序- 蛋白质相互作用和翻译后修饰。我们建议将重点放在这些方面 将信号通路与膜等主要细胞活动联系起来的机制 贩运、细胞分裂和碳代谢。此外，我们还将研究BR信号如何 与其他信号通路交叉对话，以平衡新陈代谢与增长需求，并 对植物发育中的细胞分化进行编程。这项提案中概述的研究将 继续显著提高我们对细胞信号分子机制的理解 集成和信息处理。