Genetic Biochemical Studies of Plant Steroid Signaling

植物类固醇信号传导的遗传生化研究

• 批准号: 8294084
• 负责人: ALMA L BURLINGAME
• 金额: $ 34.98万
• 依托单位: CARNEGIE INSTITUTION OF WASHINGTON, D.C.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2016-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8294084
• 关键词: Agriculture; Animals; Binding; Biochemical; Biology; Cell Differentiation process; Cell Surface Receptors; Cell surface; Cells; Computer Analysis; Data; Development; Developmental Process; Family; Foundations; GATA2 transcription factor; Gene Expression; Gene Expression Regulation; Gene Targeting; Genes; Genetic; Genetic Transcription; Genomics; Gibberellins; Goals; Growth; Health; Hormonal; Hormones; Human; Knowledge; Lead; Life Cycle Stages; Light; MAP Kinase Kinase Kinase; Mediating; Mitogen-Activated Protein Kinase Kinases; Mitogen-Activated Protein Kinases; Molecular; Nuclear; Nuclear Receptors; Pathway interactions; Phosphoric Monoester Hydrolases; Phosphotransferases; Physiological; Physiological Processes; Phytochrome; Plant Physiology; Plants; Play; Process; Proteins; Proteomics; Regulation; Research; Role; Signal Pathway; Signal Transduction; Signal Transduction Pathway; Signaling Protein; Solid; Somatotropin; Steroids; Stomas; System; biological systems; cell growth regulation; gene function; genetic analysis; improved; insight; member; mutant; plant growth/development; receptor; research study; response; transcription factor

DESCRIPTION (provided by applicant): The long-term goal of this project is to understand the molecular network through which brassinosteroid (BR) and other hormonal and environmental signals together control plant growth and development. Steroids are important hormones in both plants and animals. BR regulates a wide range of developmental and physiological processes in plants through a signal transduction pathway from cell- surface receptor kinases (BRI1) to nuclear transcription factors (BZR1 and BZR2/BES1). BR acts together with other hormonal and environmental signals to regulate plant development and physiology, and has particularly intimate relationships with light and gibberellin (GA). We have made great progress in understanding the molecular details of how BR binding to BRI1 leads to activation of BZR1 and regulation of over a thousand target genes. Furthermore, we have gained insights into how the BR signaling pathway is wired together with other pathways to control various functions in plants. For this renewal proposal, we plan to understand how the BR signaling pathway crosstalks with the stomata receptor kinase pathway and the light and gibberellin signaling pathways to coordinate cell differentiation and plant development. We will use a combination of genetic, proteomic, and genomic approaches to achieve the following specific Aims. 1) We will study how BR signaling regulates the MAP kinases and crosstalks with the ERECTA family receptor kinases to regulate stomata differentiation. 2) We will elucidate the mechanism of integration of the BR and light signaling pathways, by characterizing the interaction between BZR1 and the phytochrome- interacting factors (PIFs) in regulating common target genes, and the functions of three BZR1-target transcription factors in light regulation of gene expression and development. 3) We will study the molecular mechanisms by which BR and GA hormones orchestrate common developmental processes. The experiments proposed in this proposal will elucidate a regulatory network that integrates four important signaling pathways controlling plant growth. This will greatly advance our understanding of not only the molecular mechanisms of signal transduction but also the mechanisms of signaling pathway integration. Although the BR signaling pathway is distinct from the nuclear receptor-mediated steroid signaling mechanism in animals, many components and signaling mechanisms used by the BR pathway are conserved and play important roles in either steroid regulated processes or other signaling pathways in animals. Thus, this study not only is important for plant biology and agriculture, but also can potentially help us understand fundamental mechanisms of steroid function and cellular regulation that are relevant to human health. PUBLIC HEALTH RELEVANCE: Steroids are important growth hormones in both animals and plants. The brassinosteroid hormone is major growth promoting hormone that control a wide range of activities in plants. Brassinosteroid signal transduction uses many proteins that are similar to important proteins in humans, and understanding the functional mechanisms of these proteins will lead to not only means for improving crop yield but also knowledge that benefit human health.

描述(申请人提供)：该项目的长期目标是了解油菜素类固醇(BR)和其他激素和环境信号共同控制植物生长和发育的分子网络。类固醇是植物和动物体内的重要荷尔蒙。BR通过从细胞表面受体激酶(BRI1)到核转录因子(BZR1和BZR2/BES1)的信号转导途径调控植物的广泛发育和生理过程。BR与其他激素和环境信号一起调节植物的发育和生理，与光和赤霉素(GA)有着特别密切的关系。我们在了解BR与BRI1结合如何导致BZR1激活和调控1000多个靶基因的分子细节方面取得了很大进展。此外，我们对BR信号通路如何与其他通路连接在一起来控制植物中的各种功能有了深入的了解。对于这一更新建议，我们计划了解BR信号通路如何与气孔受体激酶通路以及光和赤霉素信号通路相互作用，以协调细胞分化和植物发育。我们将结合使用遗传学、蛋白质组学和基因组学方法来实现以下具体目标。1)我们将研究BR信号如何与ERECTA家族受体激酶一起调节MAP激酶和串扰，从而调节气孔分化。2)通过研究BZR1与光敏色素相互作用因子(PIF)在调控共同靶基因中的相互作用，以及三个BZR1-靶转录因子在光调控基因表达和发育中的作用，阐明BR和光信号通路整合的机制。3)我们将研究BR和GA激素协调共同发育过程的分子机制。这项提案中提出的实验将阐明一个调控网络，该网络整合了四个控制植物生长的重要信号通路。这将极大地促进我们对信号转导的分子机制以及信号通路整合机制的理解。虽然BR信号通路不同于动物体内核受体介导的类固醇信号通路，但BR通路所使用的许多成分和信号机制是保守的，无论是在类固醇调节过程中还是在动物的其他信号通路中都发挥着重要作用。因此，这项研究不仅对植物生物学和农业具有重要意义，而且可能有助于我们了解与人类健康相关的类固醇功能和细胞调节的基本机制。 与公共健康相关：类固醇是动物和植物中重要的生长激素。油菜素类固醇激素是一种主要的促生长激素，控制着植物体内广泛的活动。油菜素内酯信号转导使用许多与人类重要蛋白质相似的蛋白质，了解这些蛋白质的作用机制不仅有助于提高作物产量，还将有助于人类健康。