CAREER: Novel Signaling Components For Plant Steroid Regulated Gene Expression in Arabidopsis

职业：拟南芥中植物类固醇调节基因表达的新型信号成分

• 批准号: 0546503
• 负责人: Yanhai Yin
• 金额: $ 65.22万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0546503&HistoricalAwards=false
• 关键词: CAREER; Novel; Signaling; Components; Plant

Yanhai Yin 0546503CAREER: Novel Signaling Components For Plant Steroid Regulated Gene Expression In Arabidopsis Plant steroid hormones called brassinosteroids (BRs) control many diverse processes such as cell growth, senescence and stress responses. Application of BRs has been shown to increase crop yield and improve plant resistance to drought, high temperature and various diseases. Although significant progress has been made in identifying BR receptors and several other signaling components, little is known about how plant steroids regulate gene expression, thereby controlling different biological responses. Modern genetic and genomic approaches will be used to address this question. A BR-regulated transcription factor BES1 mediates many of the hormone responses and provides a powerful tool to study BR-regulated gene expression. To reveal the gene regulatory circuits, the functions of several BES1-induced transcription factors will be determined by over-expression and knock-out technologies. To identify other signaling components involved in BR-regulated gene expression, mutant plants with altered responses to plant steroids will be isolated, the underlying genes will be identified and characterized. The results from these studies will reveal the complex network through which BRs control growth and responses to both biotic and abiotic stresses. Broader impacts: Since BRs control many different biological processes, it is highly desirable to be able to manipulate individual responses. For example, it would be beneficial to promote BR-induced growth and stress responses and to reduce BR-induced senescence. The acquired knowledge from the proposed research can be used to achieve these goals. Several BR mutants will be introduced into an undergraduate Plant Physiology laboratory course to illustrate how steroids control plant growth. In addition, students from underrepresented groups will be recruited to work on the project through the Program for Woman in Science and Engineering (PWSE) at Iowa State University. The integrated research and teaching program will therefore provide ample training opportunities for high school, college, graduate and postgraduate students.

Yanhai Yin 0546503CAREER：Novel Signaling Components For Plant Steroid Regulated Gene Expression In Arabidopsis植物类固醇激素油菜素类固醇激素（Brassinosteroids，BR）控制许多不同的过程，如细胞生长，衰老和胁迫反应。BR的应用已被证明可以增加作物产量，提高植物对干旱、高温和各种疾病的抗性。虽然在识别BR受体和其他几种信号传导成分方面取得了重大进展，但对植物类固醇如何调节基因表达，从而控制不同的生物反应知之甚少。现代遗传学和基因组学方法将用于解决这个问题。BR调节的转录因子BES 1介导许多激素反应，并为研究BR调节的基因表达提供了有力的工具。为了揭示基因调控回路，将通过过表达和敲除技术来确定几个BES 1诱导的转录因子的功能。为了鉴定参与BR调节的基因表达的其他信号传导组分，将分离对植物类固醇具有改变的响应的突变体植物，将鉴定和表征潜在的基因。这些研究结果将揭示BR控制生长和对生物和非生物胁迫反应的复杂网络。更广泛的影响：由于生物反应器控制着许多不同的生物过程，因此非常希望能够操纵个体的反应。例如，促进BR诱导的生长和应激反应以及减少BR诱导的衰老将是有益的。从拟议的研究中获得的知识可以用来实现这些目标。几个BR突变体将被引入到本科植物生理学实验室课程，以说明类固醇如何控制植物生长。此外，将通过爱荷华州州立大学的妇女科学和工程项目，招募来自代表性不足群体的学生参与该项目。因此，综合研究和教学计划将为高中，大学，研究生和研究生提供充足的培训机会。