Systems Approaches to Identify Gene Regulatory Networks in the Grasses

识别草类基因调控网络的系统方法

• 批准号: 1125620
• 负责人: Erich Grotewold
• 金额: $ 230.42万
• 依托单位: Ohio State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-01-15 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1125620&HistoricalAwards=false
• 关键词: Systems; Approaches; Identify; Gene; Regulatory

PI: Erich Grotewold (The Ohio State University)CoPIs: Andrea I Doseff (The Ohio State University and John Gray (University of Toledo)Establishing the architecture of gene regulatory networks and linking system components to agronomic traits is an emerging theme in plant systems biology. This project provides a concerted effort to comprehensively dissect the gene regulatory networks that target the metabolism of maize phenolic compounds, such as phenylpropanoids and flavonoids, of fundamental agricultural importance. This study combines the discovery of novel transcription factors (TFs) that control maize phenolic accumulation, with the identification of their interaction partners and of direct target genes for individual and TF combinations. The investigation of allelic TF preference to gene regulation will provide insights that will be applicable to grasses irrespective of ploidy level. The evolutionary conservation/divergence of identified TF-DNA complexes will be evaluated in other grasses, providing an unparalleled understanding of how evolution impacted plant gene regulatory systems, and resulting in knowledge directly applicable to cereal breeding. Together with the generation of a comprehensive and centralized maize transcription factor open reading frame (ORF) collection and antibodies to several regulatory proteins, these studies will significantly advance understanding of the regulation and evolution of one of the most important plant metabolic pathways in plants. The resources generated will be useful in accelerating the study of the regulation of other important metabolic and developmental pathways in maize and other grasses. The deployment of the data here generated through the Web-accessible knowledge base GRASSIUS (www.grassius.org) and MaizeGDB (www.maizegdb.org) will continue to ensure that the findings derived from this project will have the broadest possible dissemination.This project integrates genetics, molecular biology, biochemistry, mathematics, and bioinformatics for the comparative transcriptional genomics of phenolic compounds in grass crops. Its interdisciplinary nature provides unique opportunities for the training of students and postdocs across several disciplines. The strong history of involvement of students corresponding to underrepresented groups by the PI/co-PIs will be further expanded through the synergy provided by the interaction between Ohio State University, a land-grant university with a leading research component, and University of Toledo, a multicultural research extensive university with a strong education mission. Ongoing training will be complemented by the application of tools and resources developed here in workshops at Ohio State University and the University of Puerto Rico Mayagüez. The inception of SIGuE (Success In Graduate Education) program, specifically designed to increase the recruitment and success of underrepresented minorities in graduate education, provides a natural continuum in efforts to better connect undergraduate and graduate instruction.

PI：Erich Grotewold（俄亥俄州立大学）COPIS：Andrea I Doseff（俄亥俄州立大学和约翰·格雷（John Gray）和约翰·格雷（John Gray）（托莱多大学）建立基因调节网络的体系结构，并将系统组件与农业特质联系到农业特质与植物系统的新兴主题。基本农业重要性的化合物，例如基本农业的重要性。水平。将在其他谷物中评估已确定的TF-DNA复合物的差异，从而无与伦比地了解进化的植物基因调节系统，并导致知识直接适用于谷物的培养，并将其与全面的玉米转录因子的收集框架（ORF）的概述（ORF）的生成（ORF）进行了研究。植物中最重要的植物代谢途径之一的演变将有助于加速玉米和其他草的其他重要代谢和发育途径的调节。该项目将具有最广泛的传播。该项目将遗传学，分子生物学，生物化学，数学和生物信息学整合，以造成粒子化合物的比较转录基因组学，以跨多个学科的培训，在跨学科的培训中提供了独特的培训。 PI/co-PIs will be further expanded through the synergy provided by the interaction between Ohio State University, a land-grant university with a leading research component, and University of Toledo, a multicultural research extensive university with a strong education mission. Ongoing training will be completed by the application of tools and resources developed here in workshops at Ohio State University and the University of Puerto Rico Mayagüez. The inception of SIGuE (Success In Graduate Education)专门设计的计划是为了增加代表性不足的少数民族在研究生教育中的招聘和成功，提供了自然而然的努力，以更好地连接本科和研究生的指导。