Modulation of Plant Transcription Factor Function by Small Molecules

小分子对植物转录因子功能的调节

• 批准号: 1822343
• 负责人: Erich Grotewold
• 金额: $ 39.02万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2019-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1822343&HistoricalAwards=false
• 关键词: Modulation; Plant; Transcription; Factor; Function

The goal of this project is to reveal insights into a novel type of feedback regulation (a mechanism by which a small chemical molecule can regulate its own production) mediated by the binding of a molecule to the master protein regulator controlling the production of that molecule. The project will contribute to the scientific and professional development of postdoctoral fellows and will provide research experiences for undergraduate students in research. The project includes the development of a Summer Practical Workshop in Quantitative Biochemistry, which will benefit both undergraduate and graduate students, postdoctoral researchers, and junior faculty.In corn, the R protein is a transcription factor that activates transcription of genes encoding enzymes in the flavonoid biosynthetic pathway for purple anthocyanin pigments. Preliminary data suggest that binding of flavonoid molecules to R, through a specific protein domain (the ACT domain), modulates the protein-protein interactions and the DNA-binding abilities of the R protein, leading in turn to differential recognition of promoters in the genes involved in the flavonoid pathway. Experimental tests of this model will: 1) Establish the in vivo consequences of the interaction of R with flavonoids that have been identified to bind to the ACT; 2) Investigate ACT-small molecule structure-function relationships, by defining which other small molecules bind to the ACT, identifying the ACT residues relevant for the small molecule interaction, and establishing how general the results are for ACT domains in other plant transcription factors; and 3) Analyze in vivo the dynamic associations controlled by the ACT domain of R, by combining genetic resources with cellular approaches to investigate where and which interactions occur, in the presence and absence of flavonoids or other small molecules. The work is expected to reveal a new type of feedback regulatory mechanism (metabolite binding regulating a transcription factor responsible for synthesis of the ligand), which could serve as a model for how other small metabolites might regulate their own synthesis. This project is co-funded by the Directorate for Biological Sciences (Genetic Mechanisms Program in the Division of Molecular and Cellular Biosciences; Plant Genome Research Program in the Division of Integrative Organismal Systems) and by the Directorate for Mathematics and Physical Sciences (Chemistry of Life Processes Program in the Division of Chemistry).

该项目的目的是揭示对一种新型的反馈调节类型的见解（一种小型化学分子可以调节其自身产生的机制），该机制是由分子与控制该分子产生的主要蛋白质调节剂结合的结合所介导的。该项目将有助于博士后研究员的科学和专业发展，并将为研究生的研究经验提供研究经验。该项目包括开发定量生物化学的夏季实用研讨会，该研讨会将使本科生和研究生，博士后研究人员和初级教师受益。玉米中，R蛋白是一种转录因子，它激活了在类似于类黄酮生物合理的pepper put purpurpurpurpurpurpur purpure purpur pur的基因的转录。 初步数据表明，通过特定的蛋白质结构域（ACT结构域），类黄酮分子与R的结合调节了R蛋白的蛋白质 - 蛋白质相互作用和DNA结合能力，从而导致了类黄酮途径中涉及基因中启动子的差异识别。 该模型的实验测试将：1）建立R与已鉴定为结合该ACT的类黄酮相互作用的体内后果； 2）通过定义其他小分子与该ACT结合，识别与小分子相互作用相关的ACT残基，并确定其他植物转录因子中ACT域的一般结果如何，研究了ACT-MALL分子结构功能关系。 3）在体内分析由R的ACT结构域控制的动态关联，通过将遗传资源与细胞方法相结合，以研究在存在和不存在类黄酮或其他小分子的情况下研究何处和哪些相互作用。 预计这项工作将揭示一种新型的反馈调节机制（调节负责配体合成的转录因子的代谢产物结合），这可以作为其他小型代谢物如何调节其自身合成的模型。该项目由生物科学局（分子和细胞生物科学划分的遗传机制计划；综合有机体系统划分的植物基因组研究计划）和数学和物理科学局（化学划分的化学过程计划）共同资助。

项目成果

Should I stay or should I go? Trafficking of plant extra-nuclear transcription factors

• DOI: 10.1093/plcell/koad277
• 发表时间: 2024-01-02
• 期刊: PLANT CELL
• 影响因子: 11.6
• 作者: Marathe，Sarika;Grotewold，Erich;Otegui，Marisa S.
• 通讯作者: Otegui，Marisa S.