Genetic and Cellular Dissection of Mutualistic Plant-Microbe Symbioses in Medicago truncatula

蒺藜苜蓿植物-微生物共生体的遗传和细胞解剖

• 批准号: 1127155
• 负责人: Michael Udvardi
• 金额: $ 668.31万
• 依托单位: Noble Research Institute, LLC
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-03-15 至 2019-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1127155&HistoricalAwards=false
• 关键词: Genetic; Cellular; Dissection; Mutualistic; Plant

PI: Michael Udvardi (The Samuel Roberts Noble Foundation)CoPIs: Rujin Chen and Kiran Mysore (The Samuel Roberts Noble Foundation), Rebecca Dickstein (University of North Texas), Maria Harrison (The Boyce Thompson Institute), and Janine Sherrier (University of Delaware)Legumes have been an integral part of agricultural systems for thousands of years and are second only to grasses in importance for modern agriculture. Legume crops capacitate sustainable agriculture and global food security today because these plants establish two beneficial symbioses that minimize the requirement for energy-expensive and polluting N- and P-fertilizers. Specifically, symbiosis with nitrogen-fixing bacteria called rhizobia supplies legumes with reduced nitrogen while symbiosis with arbuscular mycorrhizal (AM) fungi supplies legumes with phosphate and other growth limiting mineral nutrients. Development and differentiation of nitrogen-fixing (NF) and AM symbioses require coordinated changes in the expression of thousands of plant and bacterial or fungal genes. While tremendous advances have been made recently in our understanding of plant-microbe signaling during the early stages of the legume-rhizobia and legume-AM fungal interactions, relatively little is known about the genetic basis of later stages of symbiosis development which have a great impact on the efficacy of these symbioses for agriculture. This project establishes a Virtual Center for Symbiosis Research consisting of six groups at four organizations that are at the cutting-edge of NF and AM research and genomic, genetic, molecular and cell biology resource development for legumes. The major objectives of the project include: the systematic discovery of new genes required for NF and AM symbiosis using established resources for forward- and reverse-genetics; the development of advanced genetic resources and databases for high-throughput gene function discovery in legumes; and the development of facile resources for cell and molecular biology in Medicago. It is the hope that this project will accelerate basic discovery and contribute to knowledge and the resource base for delivering system-level models and comprehensive understanding of NF and AM symbioses in the future.This project is trans-disciplinary and requires the application of genomics, functional genomics, and bioinformatics with the classical disciplines of genetics, molecular and cell biology, and plant physiology. Strong and novel training opportunities integral to the research plan will be provided to postdoctoral fellows, graduate and undergraduate students and technical staff at the Noble Foundation, the Boyce Thompson Institute and the Universities of Delaware and North Texas. All participating institutions have student outreach components that will be harnessed to maximize teaching and learning outcomes. In addition, this project will deliver genetic and molecular- and cell biology tools and databases to the legume research community. It will also strengthen collaborative links, not only among the project collaborators, but also to the legume community generally. The work will benefit society by helping to train the next generation of scientists and by helping to make agriculture more sustainable and environmentally-friendly. Vectors and transgenic and mutant seed will be maintained and distributed over the long term by the Noble Foundation's Molecular and Biological Materials Core Facility. Transcriptome data will be deposited and accessible through public repositories that include ArrayExpress Database (http://www.ebi.ac.uk/miamexpress), and the Medicago Gene Expression Atlas (http://mtgea.noble.org/v2/). All sequence data related to flanking sequence tags (FSTs) associated with Tnt1 insertions can be accessed through the project website at http://bioinfo4.noble.org/mutant/index.php.

Pi：Michael Udvardi(Samuel Roberts Noble Foundation)Copis：Rujin Chen和Kiran Mysore(Samuel Roberts Noble Foundation)，Rebecca Dickstein(北得克萨斯大学)，Maria Harison(博伊斯·汤普森研究所)和Janine Sherrier(特拉华大学)数千年来，豆类一直是农业系统不可分割的一部分，对现代农业的重要性仅次于牧草。今天，豆类作物使可持续农业和全球粮食安全具有能力，因为这些植物建立了两种有益的共生关系，最大限度地减少了对能源昂贵且污染严重的氮肥和磷肥的需求。具体地说，与根瘤菌的共生为豆类提供了还原的氮，而与丛枝菌根(AM)真菌的共生为豆类提供了磷酸盐和其他限制生长的矿物养分。固氮和AM共生体的发育和分化需要数千个植物和细菌或真菌基因表达的协调变化。虽然最近我们对豆科植物-根瘤菌和豆科植物-AM真菌相互作用早期阶段的植物-微生物信号的理解取得了巨大的进展，但对共生发育后期的遗传基础知之甚少，这对这些共生生物在农业上的有效性有很大影响。该项目建立了一个虚拟共生研究中心，由四个组织的六个小组组成，这些组织处于豆科植物核素和AM研究以及基因组、遗传、分子和细胞生物学资源开发的前沿。该项目的主要目标包括：利用已建立的正向和反向遗传学资源，系统地发现核因子和AM共生所需的新基因；开发先进的遗传资源和用于高通量基因功能发现的数据库；以及开发用于紫花苜蓿细胞和分子生物学的简易资源。希望这个项目能够加速基础发现，并为未来提供系统级模型和全面了解核因子和AM共生的知识和资源库做出贡献。该项目是跨学科的，需要将基因组学、功能基因组学和生物信息学与遗传学、分子和细胞生物学、植物生理学等经典学科相结合。将向诺布尔基金会、博伊斯·汤普森研究所、特拉华大学和北得克萨斯大学的博士后研究员、研究生和本科生以及技术人员提供强有力的和新颖的研究计划不可或缺的培训机会。所有参与的机构都有学生外联部分，将利用这些部分来最大限度地提高教学成果。此外，该项目将向豆科研究社区提供遗传、分子和细胞生物学工具和数据库。它还将加强合作联系，不仅是项目合作者之间的联系，而且是与豆类社区的联系。这项工作将有助于培养下一代科学家，并有助于使农业更加可持续和环境友好，从而造福社会。载体、转基因和突变种子将由诺布尔基金会的分子和生物材料核心设施长期维护和分发。转录组数据将通过公共储存库存放和访问，公共储存库包括ArrayExpress数据库(http://www.ebi.ac.uk/miamexpress)，和Medicago基因表达图谱(http://mtgea.noble.org/v2/).与TNT1插入相关的侧翼序列标签(FST)相关的所有序列数据都可以通过项目网站http://bioinfo4.noble.org/mutant/index.php.获取