High-Speed Two Dimensional Separations Using Micro Free Flow Electrophoresis

使用微自由流电泳进行高速二维分离

• 批准号: 1152022
• 负责人: Michael Bowser
• 金额: $ 50万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-10-01 至 2015-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1152022&HistoricalAwards=false
• 关键词: Speed; Two; Dimensional; Separations; Using

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, Professor Michael Bowser at University of Minnesota-Twin Cities and his group will use micro free flow electrophoresis (uFFE) as the second stage in 2D separations. FFE is a continuous analysis technique capable of further separating analyte peaks as they elute off the first separation column. Continuous analysis removes limitations encountered in previous 2D separations caused by under sampling. Coupling with capillary electrophoresis (CE) or nano liquid chromatography (nLC) is expected to be straightforward with no complicated valving or timing required. It is anticipated that coupling with uFFE will increase the peak capacity of these techniques 20 to 30-fold with no sacrifice of efficiency or separation time in the first dimension separation. Total 2D peak capacities are expected to reach 2,000 in separation times of 15 min. Experiments are planned to fully characterize the peak capacity and compatibility of the many combinations of separation modes possible using this approach. As a demonstration of the potential power of high speed 2D separations CE-uFFE will be incorporated into online microdialysis assays for measuring in vivo neurochemical dynamics. Online microdialysis requires high-speed separations of complex mixtures, making this assay an ideal application to demonstrate the advantages of the 2D separations developed in this proposal. The technical benefit of the proposed approach lies in its increased peak capacity without sacrificing separation time. 2D uFFE therefore has the potential to be adopted widely, improving research capabilities and efficiency in many research areas. Beyond practical advantages, exploration into 2D-uFFE will provide unique insight into the fundamentals of separation science. The researchers at UM-twin cities also intend to design the research project described in this proposal with the goal of maximizing its impact on society through intellectual enrichment, mentoring and training opportunities, inclusion of underrepresented groups and infrastructure enhancement.

在化学系化学测量和成像项目的支持下，明尼苏达大学双城分校的Michael鲍泽教授及其团队将使用微自由流电泳（uFFE）作为2D分离的第二阶段。FFE是一种连续分析技术，能够在分析物峰从第一分离柱分离时进一步分离分析物峰。连续分析消除了在以前的2D分离中遇到的由采样不足引起的限制。与毛细管电泳（CE）或纳米液相色谱（nLC）的耦合预计是直接的，不需要复杂的阀门或定时。预期与uFFE的耦合将使这些技术的峰值容量增加20至30倍，而不会牺牲第一维分离中的效率或分离时间。总的2D峰值容量预计将达到2,000分离时间为15分钟。实验计划充分表征峰值容量和兼容性的许多组合的分离模式可能使用这种方法。作为高速2D分离的潜在能力的证明，CE-uFFE将被纳入在线微透析测定中，用于测量体内神经化学动力学。在线微透析需要高速分离复杂的混合物，使该分析成为展示该提案中开发的2D分离优势的理想应用。所提出的方法的技术优势在于其在不牺牲分离时间的情况下增加了峰值容量。因此，2D uFFE具有被广泛采用的潜力，可以提高许多研究领域的研究能力和效率。除了实际优势，对2D-uFFE的探索将为分离科学的基础提供独特的见解。UM双城的研究人员还打算设计本提案中描述的研究项目，其目标是通过知识丰富，指导和培训机会，纳入代表性不足的群体和基础设施改善来最大限度地提高其对社会的影响。