Label-Free Electrochemical Capacitance DNA Sensing with Passive Wireless Radio Frequency Identification Technology

采用无源无线射频识别技术的无标记电化学电容 DNA 传感

• 批准号: 1152940
• 负责人: Carlos Cabrera
• 金额: $ 36万
• 依托单位: University of Puerto Rico-Rio Piedras
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-15 至 2017-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1152940&HistoricalAwards=false
• 关键词: Label; Free; Electrochemical; Capacitance; DNA

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, and co-funding from the Office of Experimental Program to Stimulate Competitive Research (EPSCoR), Professors Carlos R. Cabrera at University of Puerto Rico, Rio Piedras, Professor Yi Jia at University of Puerto Rico - Mayaguez and their groups are developing a Label-Free Electrochemical Capacitance DNA Sensing with Passive Wireless Radio Frequency Identification Sensor Technology. Detection and characterization of DNA immobilization and hybridization processes are important in many applications including environmental monitoring and biomedical sensing. Current techniques for DNA detection and characterization often requires sophisticated equipment and complicated sample treatment. The supported research focuses studies on DNA capacitive sensing mechanism and the DNA/surface interfacial conformational characterization during DNA immobilization and hybridization processes with passive wireless integrated RFID sensor technologies, with the aims of developing an innovative, low cost lab-on-a-chip DNA sensing technology.The development of the proposed low-cost, label-free DNA sensing technology will enable the use of the device for different diseases (e.g. tuberculosis) or hazardous materials (e.g. anthrax) detection at point of care, such as at home or clinic. The proof-of-concept preparation of the label-free wireless RFID biosensor will create spin-offs that may benefit our students and society. In addition to the specific scientific contribution and technological advances that will be the direct result of the proposed research, this work will improve human society by broadening the participation of underrepresented groups in an important area of biosensor technology.This project will benefit the University of Puerto Rico by reinforcing existing biosensor research and educational programs in the areas of wireless sensor and integrated sensing technology, provide research opportunities for undergraduate and graduate students, and enhance competitive research capability at UPR. The project will also impact the society by disseminating the new scientific knowledge, technological innovation, and educational course materials through conferences, journals, project website and open house for K-12 students and teachers. The students participating in the proposed project will be of Puerto Rican origin, and thus the requested funding will lead to an increase in the number of Hispanic and women students pursuing advanced degrees and professional careers in science and engineering.

在化学系化学测量和成像项目的支持下，并由实验项目办公室共同资助，以刺激竞争性研究（EPSCoR），教授卡洛斯R。波多黎各大学的Cabrera，Rio Piedras，波多黎各大学的Yi Jia教授和他们的小组正在开发一种无标记电化学电容DNA传感与无源无线射频识别传感器技术。DNA固定和杂交过程的检测和表征在许多应用中是重要的，包括环境监测和生物医学传感。目前用于DNA检测和表征的技术通常需要复杂的设备和复杂的样品处理。该研究重点是利用无源无线集成RFID传感器技术研究DNA电容传感机理和DNA固定和杂交过程中DNA/表面界面构象表征，旨在开发创新的低成本芯片实验室DNA传感技术。无标记DNA传感技术将使该设备能够在护理点（例如在家中或诊所）用于不同疾病（例如肺结核）或危险材料（例如炭疽）检测。无标签无线RFID生物传感器的概念验证准备将产生副产品，可能有利于我们的学生和社会。除了具体的科学贡献和技术进步，这将是拟议的研究的直接结果，这项工作将改善人类社会，扩大参与的代表性不足的群体在一个重要领域的生物传感器技术。该项目将有利于波多黎各大学加强现有的生物传感器研究和教育计划，在无线传感器和集成传感技术领域，为本科生和研究生提供研究机会，并提高普遍定期审议的竞争力。该项目还将通过会议、期刊、项目网站和面向K-12学生和教师的开放日，传播新的科学知识、技术创新和教育课程材料，从而对社会产生影响。参加拟议项目的学生将是波多黎各裔，因此，所要求的资金将导致更多的西班牙裔和女学生攻读科学和工程方面的高级学位和职业生涯。