Rapid, Selective, Onsite Detection of Bacterial Pathogens Using A Bioinspired Microfluidic Sensor

本研究的目的是建立一种高通量的微流控生物传感器，用于快速，现场，高选择性检测各种食品和水传播的细菌病原体。该方法是采用一种创新的生物素-链霉亲和素为基础的免疫亲和识别方法的真实的时间细菌病原体的识别，和一个多路复用的并行传感阵列的快速检测。由于传感器的高通量、高选择性和高灵敏度，其智力价值是能够实现用于细菌病原体快速分析的变革性实验工具。 基于链霉亲和素-生物素相互作用的免疫亲和识别是无标记的，仅需要用生物素进行一次通用的表面修饰，并且可以用于检测许多细菌病原体。因此，它将导致低成本的细菌病原体和细胞的现场检测的变革性技术。对平行微流通道信号多路复用的研究将为开发高通量生物传感器提供一个通用平台。 更广泛的影响是多方面的。拟议的研究将对食品和饮用水安全监测、环境监测、生物战检测和临床诊断产生变革性影响。 该项目将为参与研究的研究生和本科生提供跨学科培训的机会。该项目还旨在开发芯片实验室和生物纳米技术相关的教育材料，并有助于激励本科生和K-12学生追求科学和工程职业。通过阿克伦大学？s？增加工程学术的多样性？，?女性在工程？然后呢？高中桥？本计画将招募研究生、本科生及高中生进行研究。

The objective of this research is to create a high-throughput microfluidic biosensor for rapid, onsite, highly-selective detection of a variety of food and water borne bacterial pathogens. The approach is to employ an innovative biotin-streptavidin based immunoaffinity recognition method for real time bacterial pathogen recognition, and a multiplexed parallel sensing array for rapid detection. The intellectual merit is to enable a transformative experimental tool for rapid analysis of bacterial pathogens because of the high throughput, high selectivity and high sensitivity of the sensor. The immunoaffinity recognition based on streptavidin-biotin interaction is label free, requires only one universal surface modification with biotin, and can be used for detecting many bacterial pathogens. Thus it will lead to a transformative technology for low cost, onsite detection of bacterial pathogens and cells. Research on multiplexation of signals from parallel microfluidic channels will yield a generalized platform for developing high throughput biosensors. The broader Impacts are multifold. The proposed research will have transformative impacts on food and drinking water safety monitoring, environmental monitoring, bio-warfare detection, and clinical diagnosis. This project will provide interdisciplinary training opportunity for the graduate and undergraduate students involved in the research. The project also aims to develop lab-on-a-chip and bio-nanotechnology related educational materials and contribute to motivating undergraduate students and K-12 students to pursue science and engineering careers. Through University of Akron?s ?Increasing Diversity in Engineering Academics?, ?Women in Engineering? and ?High School Bridge? programs, undergraduate students and high school students will be recruited to conduct research in this project.