Rapid, Selective, Onsite Detection of Bacterial Pathogens Using A Bioinspired Microfluidic Sensor

这项研究的目的是创建一种高通量微流控生物传感器，用于快速、现场、高选择性地检测各种食品和水传播的细菌病原体。该方法是使用一种创新的基于生物素-链霉亲和素的免疫亲和识别方法来实时识别细菌病原体，并使用多路并行传感阵列来快速检测。由于传感器的高通量、高选择性和高灵敏度，智能的优点是使快速分析细菌病原体的实验工具成为可能。基于链霉亲和素-生物素相互作用的免疫亲和识别无需标记，只需用生物素进行一次通用的表面修饰，可用于多种细菌病原体的检测。因此，它将带来一种变革性的技术，用于低成本、现场检测细菌病原体和细胞。对并行微流控通道信号的多路复用研究将为开发高通量生物传感器提供一个通用的平台。更广泛的影响是多方面的。这项拟议的研究将对食品和饮用水安全监测、环境监测、生物战检测和临床诊断产生革命性影响。该项目将为参与研究的研究生和本科生提供跨学科的培训机会。该项目还旨在开发与芯片实验室和生物纳米技术相关的教材，并有助于激励本科生和K-12学生追求科学和工程职业。通过阿克伦大学？S？增加工程学者的多样性？？工程学中的女性？然后呢？高中桥？该项目将招募本科生和高中生进行研究。

The objective of this research is to create a high-throughput microfluidic biosensor for rapid, onsite, highly-selective detection of a variety of food and water borne bacterial pathogens. The approach is to employ an innovative biotin-streptavidin based immunoaffinity recognition method for real time bacterial pathogen recognition, and a multiplexed parallel sensing array for rapid detection. The intellectual merit is to enable a transformative experimental tool for rapid analysis of bacterial pathogens because of the high throughput, high selectivity and high sensitivity of the sensor. The immunoaffinity recognition based on streptavidin-biotin interaction is label free, requires only one universal surface modification with biotin, and can be used for detecting many bacterial pathogens. Thus it will lead to a transformative technology for low cost, onsite detection of bacterial pathogens and cells. Research on multiplexation of signals from parallel microfluidic channels will yield a generalized platform for developing high throughput biosensors. The broader Impacts are multifold. The proposed research will have transformative impacts on food and drinking water safety monitoring, environmental monitoring, bio-warfare detection, and clinical diagnosis. This project will provide interdisciplinary training opportunity for the graduate and undergraduate students involved in the research. The project also aims to develop lab-on-a-chip and bio-nanotechnology related educational materials and contribute to motivating undergraduate students and K-12 students to pursue science and engineering careers. Through University of Akron?s ?Increasing Diversity in Engineering Academics?, ?Women in Engineering? and ?High School Bridge? programs, undergraduate students and high school students will be recruited to conduct research in this project.