Natural Resin Acid-Containing Antimicrobial BioMaterials: Synthesis and Mechanism

天然树脂含酸抗菌生物材料的合成与机理

• 批准号: 1206072
• 负责人: Chuanbing Tang
• 金额: $ 42万
• 依托单位: University South Carolina Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1206072&HistoricalAwards=false
• 关键词: Natural; Resin; Acid; Containing; Antimicrobial

ID: MPS/DMR/BMAT(7623) 1206072 PI: Tang, Chuanbing ORG: University of South CarolinaTitle: Natural Resin Acid-Containing Antimicrobial BioMaterials: Synthesis and MechanismINTELLECTUAL MERIT: Bacterial infections remain one of the most serious complications leading to destruction of local tissues, patient disability, morbidity, and death. This project seeks to develop low-cost biomaterials and antimicrobial agents that kill a broad spectrum of bacteria, but present low toxicity to mammalian cells. It is proposed that natural resin acid-based cationic materials, which can be synthesized in large quantities and at low cost, can be used as robust, selective antimicrobial agents to treat various bacterial infections or incorporated into plastics such as those used in medical catheters. Preliminary data show that natural resin acids, when chemically-modified to acid-derived cationic compounds and polymers, efficiently inhibit growth of a wide range of Gram-positive and Gram-negative bacteria, without inducing significant hemolysis of red blood cells. The PI will demonstrate the versatility of resin acid-derived cationic compounds and polymers with controlled charge location, charge density, balanced hydrophobicity, and unique conformation via precise molecular and macromolecular design. The project will combine fluorescence microscopy/spectroscopy and atomistic molecular dynamics modeling to study interactions between these materials and bacterial membranes. These unique materials will be formulated into antimicrobial coatings on medical implants based on titanium and polymers. The ultimate goal is to translate the most desirable and unique properties of resin acid-based cationic compounds and polymers into new classes of antimicrobial agents with functionality and performance that are unprecedented for current antimicrobial materials. BROADER IMPACTS: Infections associated with bacterial contamination are a critical issue in global healthcare. This proposal advances not only the knowledge of resin acids via the unprecedented use of chemistry and polymerization techniques, but also uncovers new concepts for utilizing hydrophobic resin acids as active components in antimicrobial agents. The research proposed herein will have a positive impact on reducing the escalating occurrences of bacterial infection. The proposed investigations cut across the fields of synthetic organic and polymer chemistry, microbiology, and biomedicine. The technical nature of this proposal will provide training opportunities for graduate and undergraduate students in polymer synthesis and antimicrobials. An important component of the above plan involves several integrated educational activities for high school, undergraduate, and graduate students to stimulate their interest in science and technology with enhanced global research and education training opportunities. An international outreach program for Education in Sustainable Materials and Nanotechnologies will be pursued with a Chinese institution to facilitate global cooperation/networking in addressing natural product utilization and global healthcare and to strengthen the international competence and competitiveness of American students. Furthermore, the project will participate in the ACS Seed program to support summer research opportunities for high school students from economically disadvantaged backgrounds. Existing collaborations with Historically Black Colleges and Universities will continue to train African American undergraduate students. Finally, a Young Scientist Program, which was initiated over a decade ago through NSF funding, will be continued at local Primary and Middle schools.

ID：MPS/DMR/BMAT（7623）1206072 主要研究者：唐传兵 ORG：南卡罗来纳大学天然树脂含酸抗菌生物材料：合成和机理知识：细菌感染仍然是最严重的并发症之一，导致局部组织破坏，患者残疾，发病和死亡。 该项目旨在开发低成本的生物材料和抗菌剂，杀死广谱细菌，但对哺乳动物细胞的毒性较低。 提出了可以大量且低成本合成的天然树脂酸基阳离子材料可以用作稳健的选择性抗微生物剂以治疗各种细菌感染或掺入塑料中，例如用于医疗导管中的那些。 初步数据表明，天然树脂酸，当化学改性为酸衍生的阳离子化合物和聚合物时，有效地抑制了广泛的革兰氏阳性和革兰氏阴性细菌的生长，而不诱导红细胞的显著溶血。 PI将通过精确的分子和大分子设计展示树脂酸衍生的阳离子化合物和聚合物的多功能性，这些化合物和聚合物具有受控的电荷位置、电荷密度、平衡的疏水性和独特的构象。 该项目将结合联合收割机荧光显微镜/光谱学和原子分子动力学模型来研究这些材料和细菌膜之间的相互作用。 这些独特的材料将被配制成基于钛和聚合物的医疗植入物上的抗菌涂层。 最终目标是将基于树脂酸的阳离子化合物和聚合物的最理想和独特的性质转化为具有当前抗菌材料前所未有的功能和性能的新型抗菌剂。更广泛的影响：与细菌污染相关的感染是全球医疗保健中的一个关键问题。 该提案不仅通过前所未有的化学和聚合技术的使用推进了树脂酸的知识，而且还揭示了利用疏水性树脂酸作为抗菌剂活性组分的新概念。 本文提出的研究将对减少细菌感染的不断升级产生积极影响。 拟议的调查跨越合成有机和高分子化学，微生物学和生物医学领域。 该提案的技术性质将为研究生和本科生提供聚合物合成和抗菌剂方面的培训机会。 上述计划的一个重要组成部分是为高中生、本科生和研究生举办几项综合教育活动，以激发他们对科学和技术的兴趣，增加全球研究和教育培训机会。 将与一家中国机构开展可持续材料和纳米技术教育的国际推广计划，以促进天然产品利用和全球医疗保健方面的全球合作/网络，并加强美国学生的国际能力和竞争力。 此外，该项目将参与ACS种子计划，以支持来自经济困难背景的高中生的夏季研究机会。 与传统黑人学院和大学的现有合作将继续培训非裔美国本科生。 最后，十多年前通过国家科学基金会资助发起的青年科学家计划将在当地中小学继续实施。