EAGER: Complete 3D imaging of needle biopsy to diagnose pancreatic cancer

[1212540SeibelA]提出了一种新的处理和成像方法，用于胰腺癌（动物和人类）切除组织的薄针核心活检（TNCB）。0.25mm直径的TNCB标本将通过定制的取芯装置获得，而更大的0.40mm将使用新的商业活检针进行取芯。相应的细针抽吸（FNA）标本将在TNCB样本处或旁边采集。在将TNCB组织保持在管或微流体腔内的同时，将标本固定、染色和光学清除。苏木精和伊红（H&amp；E）的吸收染料将为细胞和组织的形态结构提供透射图像对比度。荧光染料将从各种免疫组织学目标成像，例如在基础上皮膜上表达的蛋白质，以帮助确定入侵和蛋白质表达。光学投影层析显微镜（OPTM）将用于生成活检组织的3D多模态图像。虽然组织核心的长度会比显微镜视场（FOV）长，但组织的不同区域将被成像并通过图像处理拼接在一起。整个TNCB标本的2D和3D可视化将提供给病理学家，使用定制的计算机界面来操作3D数据集。肿瘤诊断的敏感性和特异性将以组织切片病理为金标准。将比较评估OPTM与传统FNA成像的TNCB对胰腺癌的诊断。这项新技术不仅可以检测肿瘤细胞的存在，还可以检测癌细胞对组织的侵袭。

1212540SeibelA new method for processing and imaging of thin-needle core biopsy (TNCB) of resected tissue containing pancreatic cancer (animal and human) is proposed. TNCB specimens of 0.25mm diameter will be obtained by a custom-designed coring device, while larger 0.40mm cores will be taken with a new commercial biopsy needle. Corresponding fine needle aspirate (FNA) specimens will be taken at or alongside the TNCB sample sites. While maintaining TNCB tissue within a tube or microfluidic chamber, the specimen will be fixed, stained, and optically cleared. Absorption dyes of hematoxylin and eosin (H&E) will provide transmission image contrast for the morphological structure of cells and tissue.Fluorescence dye will be imaged from various immunohistological targets, such as proteins expressed at the base epithelial membrane to help determine invasion and protein expression. Optical projection tomographic microscopy (OPTM) will be used to generate 3D multimodal images of biopsy tissue. Although the length of the tissue core will be longer than the microscope field of view (FOV), different regions of the tissue will be imaged and stitched together by image processing. Both 2D and 3D visualization of the entire TNCB specimen will be provided to a pathologist using a custom computer interface to manipulate the 3D dataset. Sensitivity and specificity of cancer diagnosis will be made using tissue-slice pathology as the gold standard. Comparative evaluation of pancreatic cancer diagnosis will be made between TNCB imaged with the OPTM versus conventional FNA. This new technique is expected not only to detect the presence of neoplastic cells but detect invasion of tissue by cancer cells.