EAGER: Complete 3D imaging of needle biopsy to diagnose pancreatic cancer

EAGER：完整的针活检 3D 成像诊断胰腺癌

• 批准号: 1212540
• 负责人: Eric Seibel
• 金额: $ 30万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-03-15 至 2015-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1212540&HistoricalAwards=false
• 关键词: EAGER; Complete; 3D; imaging; needle

1212540SeibelA new method for processing and imaging of thin-needle core biopsy (TNCB) of resected tissue containing pancreatic cancer (animal and human) is proposed. TNCB specimens of 0.25mm diameter will be obtained by a custom-designed coring device, while larger 0.40mm cores will be taken with a new commercial biopsy needle. Corresponding fine needle aspirate (FNA) specimens will be taken at or alongside the TNCB sample sites. While maintaining TNCB tissue within a tube or microfluidic chamber, the specimen will be fixed, stained, and optically cleared. Absorption dyes of hematoxylin and eosin (H&E) will provide transmission image contrast for the morphological structure of cells and tissue.Fluorescence dye will be imaged from various immunohistological targets, such as proteins expressed at the base epithelial membrane to help determine invasion and protein expression. Optical projection tomographic microscopy (OPTM) will be used to generate 3D multimodal images of biopsy tissue. Although the length of the tissue core will be longer than the microscope field of view (FOV), different regions of the tissue will be imaged and stitched together by image processing. Both 2D and 3D visualization of the entire TNCB specimen will be provided to a pathologist using a custom computer interface to manipulate the 3D dataset. Sensitivity and specificity of cancer diagnosis will be made using tissue-slice pathology as the gold standard. Comparative evaluation of pancreatic cancer diagnosis will be made between TNCB imaged with the OPTM versus conventional FNA. This new technique is expected not only to detect the presence of neoplastic cells but detect invasion of tissue by cancer cells.

1212540Seibel提出了一种新的胰腺癌切除组织(动物和人)细针芯活检(TNCB)的处理和成像方法。直径0.25 mm的TNCB标本将通过定制设计的取芯装置获得，而更大的0.40 mm取芯将使用新的商业活检针。相应的细针抽吸物(FNA)样本将在TNCB样本点或与其一起采集。当将TNCB组织保存在试管或微流体室中时，标本将被固定、染色和光学清除。苏木精伊红吸收染料(H&Amp；E)将为细胞和组织的形态结构提供透射性图像对比。荧光染料将从各种免疫组织学目标成像，例如在基底上皮膜上表达的蛋白质，以帮助确定侵袭和蛋白质表达。光学投影断层扫描显微镜(OPTM)将用于生成活检组织的3D多模式图像。虽然组织芯的长度将比显微镜视野(FOV)更长，但组织的不同区域将通过图像处理成像并缝合在一起。整个TNCB标本的2D和3D可视化都将提供给病理学家，使用定制的计算机界面来操作3D数据集。肿瘤诊断的敏感性和特异性将以组织切片病理学为金标准。对OPTM的TNCB图像与传统的FNA对胰腺癌的诊断进行对比评价。这项新技术预计不仅可以检测肿瘤细胞的存在，还可以检测癌细胞对组织的侵袭。