RUI: Stationary phase development for chromatographic analysis of solute interactions with phospholipid membranes

RUI：用于溶质与磷脂膜相互作用的色谱分析的固定相开发

• 批准号: 1214145
• 负责人: Eric Ross
• 金额: $ 24.15万
• 依托单位: Gonzaga University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-15 至 2016-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1214145&HistoricalAwards=false
• 关键词: RUI; Stationary; phase; development; chromatographic

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, Professor Eric Ross and his group at Gonzaga University are developing new chromatography methods and materials for the evaluation of binding and partitioning events at lipid bilayers. The materials under study are based on dynamic phospholipid phases supported on aggregates of silica nanoparticles which allow lipid bilayers and incorporated synthetic and natural receptors to be assayed in a nano-volume liquid chromatography format. The high-performance features of the solid support and the dynamic properties of natural membranes that are replicated in the phospholipid coatings promise to extend the capabilities of current biomembrane affinity stationary phases to probe the influence of membrane and solution composition on the function of natural or synthetic pores and channels within lipid bialyers or to screen substances that modulate their function. Project goals include structurally and chemically optimizing the stationary phases for the support of lipid bilayers and functional embedded receptors, optimizing chromatographic procedures for studies of membrane partitioning and binding with key model receptors both natural and synthetic, understanding the separation mechanism associated with ion-channel functionalized membranes, and establishing the compatibility of the system and format with increasingly complex biomembrane systems. Advances in research-enabling, cost- and time-effective technology that can be used to investigate factors affecting the strength and selectivity of interactions at biomembranes have the potential to broadly impact scientific fields that study this crucial biological interface which is hampered by the various analytical limitations of existing model systems. Engagement of undergraduate students in all aspects of the research project beginning as early as the sophomore year and continuing through graduation will provide structured and advanced training in applied materials and bioanalytical sciences for students largely from rural areas of the inland Northwest. Multi-year engagement on collaborative projects will allow undergraduate researchers to engage in vertical mentorship roles as they grow in scientific skill and ability.

在化学系化学测量和成像项目的支持下，贡扎加大学的Eric Ross教授和他的团队正在开发新的色谱方法和材料，用于评估脂质双层的结合和分配事件。 正在研究的材料是基于动态磷脂相支持的二氧化硅纳米粒子的聚集体，使脂质双层和纳入合成和天然受体进行测定的纳米体积液相色谱格式。 固体支持物的高性能特征和在磷脂涂层中复制的天然膜的动态特性有望扩展当前生物膜亲和固定相的能力，以探测膜和溶液组合物对脂质双分子内的天然或合成孔和通道的功能的影响，或筛选调节其功能的物质。 项目目标包括结构和化学优化固定相的支持脂质双层和功能嵌入受体，优化色谱程序的膜分区和结合的研究与关键模型受体天然和合成，了解与离子通道功能化膜相关的分离机制，并建立系统的兼容性和格式与日益复杂的生物膜系统。 在研究，成本和时间效益的技术，可用于调查影响生物膜的相互作用的强度和选择性的因素的进展有可能广泛影响科学领域，研究这一关键的生物界面，这是阻碍了现有模型系统的各种分析限制。 本科生参与研究项目的各个方面，早在大二开始，并继续通过毕业将提供应用材料和生物分析科学的结构化和高级培训，主要是来自西北内陆农村地区的学生。 合作项目的多年参与将使本科生研究人员在科学技能和能力增长时能够参与垂直导师角色。