MRI: Acquisition of a high resolution Q-TOF Mass Spectrometer

通过这项来自主要研究仪器（MRI）项目的奖励，来自纽约市立大学亨特学院的Mande Holford教授及其同事Charles Drain和Clifford Soll将获得一台高清四极杆飞行时间（Q-TOF）液相色谱质谱计（LC/MS）。该提案旨在加强各级的研究培训和教育，特别是在以下领域：(a)小分子；(b)生物聚合物;(c)从复杂混合物中发现天然产物；(d)组合库；(e)涉及完整蛋白质或酶消化的生物物理学；(f)纳米材料支架；(g)功能化脂质和染料。质谱法通过多种方法使样品蒸发和电离。粒子（母离子和碎片离子）流入质谱仪，在质谱仪中测量它们的质量/电荷比。这种高度灵敏的技术可以测定粒子的质量，它们的化学成分，并可以推断出复杂混合物中物质的结构。LC/MS结合了液相色谱的物理分离能力和质谱的质量分析能力。液相色谱仪将混合物分离成分子组分。这些成分流入质谱仪，在那里它们被电离成母离子和它的碎片离子，并测量它们的质量。该奖项提供的仪器将为教师和学生提供在纽约市立大学亨特学院进行高级研究项目的机会。它将供纽约市立大学系统各校区的教师和学生使用。它将促进许多学生在使用这一重要且广泛应用的分析技术方面的培训，并将促进化学、材料研究、纳米技术和生物化学的研究。

With this award from the Major Research Instrumentation (MRI) program, Professor Mande Holford from CUNY Hunter College and colleagues Charles Drain and Clifford Soll will acquire a high definition quadrupole time-of-flight (Q-TOF) liquid-chromatography mass spectrometer (LC/MS). The proposal is aimed at enhancing research training and education at all levels, especially in areas such as (a) small molecules; (b) biopolymers; (c) natural products discovery from complex mixtures; (d) combinatorial libraries; (e) biophysics involving intact proteins or enzymatic digests; (f) scaffolds for nanotechnology materials; and (g) functionalized lipids and dyes.Mass spectrometry vaporizes and ionizes a sample by a variety of methods. The particles (parent and fragment ions) flow into a mass spectrometer where their mass/charge ratios are measured. This highly sensitive technique allows determination of the masses of the particles, their chemical composition and enables one to deduce the structure of the substances in a complex mixture. An LC/MS combines the physical separation capability of liquid chromatography with the mass analysis ability of mass spectrometry. The liquid chromatograph separates a mixture into its molecular components. These components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. The instrumentation provided by this award will provide faculty and students the opportunity to pursue advanced research projects at CUNY-Hunter College. It will be available for use to faculty and students across the campuses in the CUNY system. It will advance the training of many students in the use of this important and widely employed analytical technique and will advance research in chemistry, materials research, nanotechnology and biochemistry.