MRI: Acquisition of a high resolution Q-TOF Mass Spectrometer

CUNY Hunter College的Mande Holford教授及其同事Charles DRAIN和Clifford Soll将获得这一来自主要研究仪器(MRI)计划的奖项，他们将获得一台高清晰度四极杆飞行时间(Q-TOF)液相色谱质谱计(LC/MS)。该提案旨在加强所有各级的研究、培训和教育，特别是在以下领域：(A)小分子；(B)生物聚合物；(C)从复杂混合物中发现天然产物；(D)组合库；(E)涉及完整蛋白质或酶消化的生物物理学；(F)纳米技术材料的支架；以及(G)功能化脂类和染料。粒子(母体离子和碎片离子)流入质谱仪，在那里测量它们的质量/电荷比。这种高度灵敏的技术可以确定颗粒的质量及其化学成分，并使人们能够推断复杂混合物中物质的结构。LC/MS结合了液相色谱的物理分离能力和质谱学的质量分析能力。液体色谱仪将混合物分离成其分子组分。这些组分流入质谱仪，在那里它们被电离成母离子，并测量其碎片离子及其质量。该奖项提供的工具将为教职员工和学生提供在纽约州立大学亨特学院进行高级研究项目的机会。它将在CUNY系统中供校园内的教职员工和学生使用。它将促进对许多学生使用这一重要和广泛使用的分析技术的培训，并将促进化学、材料研究、纳米技术和生物化学的研究。

With this award from the Major Research Instrumentation (MRI) program, Professor Mande Holford from CUNY Hunter College and colleagues Charles Drain and Clifford Soll will acquire a high definition quadrupole time-of-flight (Q-TOF) liquid-chromatography mass spectrometer (LC/MS). The proposal is aimed at enhancing research training and education at all levels, especially in areas such as (a) small molecules; (b) biopolymers; (c) natural products discovery from complex mixtures; (d) combinatorial libraries; (e) biophysics involving intact proteins or enzymatic digests; (f) scaffolds for nanotechnology materials; and (g) functionalized lipids and dyes.Mass spectrometry vaporizes and ionizes a sample by a variety of methods. The particles (parent and fragment ions) flow into a mass spectrometer where their mass/charge ratios are measured. This highly sensitive technique allows determination of the masses of the particles, their chemical composition and enables one to deduce the structure of the substances in a complex mixture. An LC/MS combines the physical separation capability of liquid chromatography with the mass analysis ability of mass spectrometry. The liquid chromatograph separates a mixture into its molecular components. These components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. The instrumentation provided by this award will provide faculty and students the opportunity to pursue advanced research projects at CUNY-Hunter College. It will be available for use to faculty and students across the campuses in the CUNY system. It will advance the training of many students in the use of this important and widely employed analytical technique and will advance research in chemistry, materials research, nanotechnology and biochemistry.