An Interdisciplinary Approach to Deciphering the Molecular Dialogue between the Plasma Membrane and Nucleus of Medicago truncatula

破译蒺藜苜蓿质膜和细胞核之间分子对话的跨学科方法

• 批准号: 1237936
• 负责人: Michael Sussman
• 金额: $ 338.49万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1237936&HistoricalAwards=false
• 关键词: Interdisciplinary; Approach; Deciphering; Molecular; Dialogue

PI: Michael R. Sussman (University of Wisconsin - Madison)CoPIs: Jean Michel Ané, Joshua J. Coon, and Sushmita Roy (University of Wisconsin - Madison)This goal of this project is to develop and apply genomic technologies for understanding at a molecular level, how bacteria and plants communicate to create a remarkable product of evolution, the nitrogen-fixing symbiotic nodule. An important part of this conversation between the microbe and the plant is a change in the chemical composition of proteins within the cell, i.e., in the degree by which phosphate groups are added or removed to specific amino acids. Using state of the art mass spectrometers to determine precisely which proteins are being modified by phosphate, and by how much in the first hour in which the bacteria meets the plant, the project will attempt to elucidate how the plant changes its proteins in order to initiate all of the myriad changes in their metabolism and gene expression that are required for the symbiotic nodules to form. All project outcomes (e.g., data, peptides and germplasm resources) will be provided to the public free of charge and without intellectual property restrictions. Medicago transcriptome, proteome and phosphoproteome data generated in this project will be deposited and accessible to the public at long-term repositories such as National Center for Biotechnology Information (NCBI: http://www.ncbi.nlm.nih.gov/), the Legume Information Service (LIS: http://www.comparative-legumes.org/) and the PeptideAtlas (www.peptideatlas.org). The mass spectrometric technology being developed and used in this project could be useful for understanding many different biological events, besides symbiotic nitrogen fixation. In order to provide opportunities for researchers all over the U.S. to access this sophisticated instrumentation and technology for use in their research, the project will develop and hold a one-week long Plant Proteomic Workshop. To be held each summer at the University of Wisconsin, this workshop will provide two dozen participants (graduate students, postdoctoral researchers and professors) with hands-on, in-depth use of mass spectrometers for measuring changes in the proteins from plants under study in their own labs. This workshop will greatly facilitate the more widespread accessibility and use of this technology for research in the general area of plant biology, and should greatly help this country's goal of training our future plant scientists and help them become comfortable with modern genomic "systems biology" experimental techniques.

PI：Michael R. Sussman（威斯康星州-麦迪逊大学）CoPI：Jean Michel Ané，约书亚J. Coon和Sushmita Roy（威斯康星州-麦迪逊大学）该项目的目标是开发和应用基因组技术，以便在分子水平上了解细菌和植物如何交流，以创造出一种显着的进化产物，固氮共生结节。微生物和植物之间这种对话的一个重要部分是细胞内蛋白质化学组成的变化，即，在磷酸基团被添加或移除到特定氨基酸的程度上。使用最先进的质谱仪来精确确定哪些蛋白质被磷酸盐修饰，以及在细菌与植物相遇的第一个小时内被修饰了多少，该项目将试图阐明植物如何改变其蛋白质，以启动共生结节形成所需的代谢和基因表达的所有无数变化。所有项目成果（例如，数据、多肽和种质资源）将免费向公众提供，不受知识产权限制。 本项目中产生的苜蓿转录组、蛋白质组和磷酸化蛋白质组数据将存放在国家生物技术信息中心（NCBI：http：//www.ncbi.nlm.nih.gov/）、豆类信息服务中心（LIS：http：//www.comparative-legumes.org/）和PeptideAtlas（www.peptideatlas.org）等长期储存库中，供公众访问。该项目正在开发和使用的质谱技术可能有助于了解许多不同的生物事件，除了共生固氮。为了给美国各地的研究人员提供机会，使他们能够使用这种先进的仪器和技术进行研究，该项目将开发并举办为期一周的植物蛋白质组学研讨会。每年夏天在威斯康星州大学举行，这个研讨会将为二十多名参与者（研究生，博士后研究人员和教授）提供实践，深入使用质谱仪测量他们自己实验室中研究的植物蛋白质的变化。这次研讨会将极大地促进更广泛地获得和使用这项技术的研究在一般领域的植物生物学，并应大大有助于我国的目标，培养我们未来的植物科学家，并帮助他们成为舒适的现代基因组“系统生物学”的实验技术。