TRTech-PGR: A mass spectrometric-based interdisciplinary approach to deciphering the molecular dialogue between between crop plants and their microbial friends and foes.

TRTech-PGR：一种基于质谱的跨学科方法，用于破译作物与其微生物朋友和敌人之间的分子对话。

• 批准号: 2010789
• 负责人: Michael Sussman
• 金额: $ 302.7万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-15 至 2024-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2010789&HistoricalAwards=false
• 关键词: TRTech; PGR; mass; spectrometric; based

In soil, the roots of crop plants interact with bacteria and fungi that play interconnected, fundamental roles in agriculture in a process known as ‘symbiosis’ whereby the plants and microbes feed each other with essential nutrients. This interdependent relationship of soil microbes and crop roots provides important elements such as nitrogen and phosphorous to the plant. Without these microbes, farmers must use fertilizers to satisfy the plant’s need for nutrients. Fertilizer use is both financially expensive and environmentally deleterious because the runoff from agricultural fields causes overfertilization of the nation’s waterways. This, in turn, results in disastrous overgrowth of unwanted organisms and large die-offs of desirable organisms like fish and other creatures living in rivers, lakes and oceans. In this project, the essential chemical signals that soil microbes use to tell a plant that they are friends, not foes, so they can live in harmony rather than at war, will be analyzed at a molecular level. In addition, during this project, the proteins in the plant that specifically recognize these signals will be characterized at the molecular level. In general, these signals represent a chemical ‘internet’ in the soil that allows plants and microbes to satisfy their critical roles in modern agriculture. This project will provide a potential strategy for increasing crop productivity needed to nourish a growing population, while decreasing the deleterious financial and environmental costs. In addition, the team will continue to provide an annual course at the University of Wisconsin on advanced methods in the use of mass spectrometry and proteomics.Mass spectrometers play a fundamental role in modern research, as well as in the health and forensics industry. For example, there is a mass spectrometer on Mars looking for signs of life, there is one at the airport that looks for terrorism-related chemicals in our bags and body, and there are mass spectrometers in our hospitals that analyze the presence and metabolism of drugs in our blood and bodily secretions. Despite their importance, most lay citizens and modern research scientists have a poor understanding of what these instruments can do and why. This renewal project is devoted to developing and applying state-of-the-art mass spectrometric technologies to profile early molecular events involved in the recognition of symbiotic microbes with their host crop plants. This project will also assist the general plant community with understanding and using mass spectrometric based analyses of metabolomes and proteomes involved in the plant's ability to recognize and respond to microbes. Finally, via continuation of the annual summer Mass Spectrometry Workshop previously established with prior PGRP NSF funding at UW-Madison, this renewal project will be able to ensure that the mass spectrometric-based technologies being developed will be made available and placed in the hands of the research community's biologists in plant and microbial laboratories throughout the USA.This award was co-funded by the Plant Genome Research Program and the Plant Biotic Interactions Program in the Division of Integrative Organismal Systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在土壤中，作物植物的根与细菌和真菌相互作用，在称为“共生”的过程中扮演着相互联系的基本角色，从而使植物和微生物互相喂食必要的营养素。土壤微生物和作物根的这种相互依存关系提供了重要元素，例如氮和磷对植物的磷。没有这些微生物，农民必须使用肥料来满足植物对营养的需求。使用肥料在财务上既昂贵又具有环境缺失，因为农田的径流导致该国水道过度利用。反过来，这导致了不必要的生物和诸如鱼类和其他生活在河流，湖泊和海洋中的其他物质（如鱼类和其他物质）的大量生物的灾难过度。在这个项目中，土壤微生物用来告诉植物是朋友而不是敌人的基本化学信号，因此他们可以在分子水平上分析它们，而不是和谐而不是战争。此外，在该项目中，植物中专门识别这些信号的蛋白质将在分子水平上进行表征。通常，这些信号代表土壤中的化学“互联网”，使植物和微生物可以满足其在现代农业中的关键作用。该项目将提供一种潜在的策略，以增加养育人口增长所需的农作物产量，同时降低微妙的财务和环境成本。此外，该团队将继续在威斯康星大学提供有关质谱和蛋白质组学使用高级方法的年度课程。质谱仪在现代研究以及健康和取证行业中起着基本作用。例如，火星上有一个质谱仪寻找生命的迹象，在我们的袋子和身体中，机场有一个在寻找与恐怖主义有关的化学物质，并且我们的医院中有质谱仪分析我们的血液和身体分泌物中药物的存在和代谢。尽管它们的重要性，但大多数外行公民和现代研究科学家对这些工具的作用和原因都有很差的了解。该更新项目致力于开发和应用最先进的质谱技术，以介绍与宿主作物植物识别共生微生物有关的早期分子事件。该项目还将帮助一般植物社区了解并使用基于质谱的基于质谱的分析，对植物识别和反应微生物的能力涉及的代谢组和蛋白质。最后，通过继续在UW-Madison的PGRP NSF资助之前建立的年度夏季质谱研讨会，该更新项目将能够确保开发基于质谱的技术，并将其在USA且基因研究中的生物学研究中提供，并将其置于研究社区的工厂研究中。综合有机体系统。该奖项反映了NSF的法定使命，并通过使用基金会的知识分子优点和更广泛的影响审查标准来评估被认为是宝贵的支持。

项目成果

Perception of lipo-chitooligosaccharides by the bioenergy crop Populus

• DOI: 10.1080/15592324.2021.1903758
• 发表时间: 2021-04-03
• 期刊: PLANT SIGNALING & BEHAVIOR
• 影响因子: 2.9
• 作者: Cope, Kevin R.;Irving, Thomas B.;Ane, Jean-Michel
• 通讯作者: Ane, Jean-Michel

High-end ion mobility mass spectrometry: A current review of analytical capacity in omics applications and structural investigations

• DOI: 10.1016/j.trac.2022.116761
• 发表时间: 2022-09-02
• 期刊: TRAC-TRENDS IN ANALYTICAL CHEMISTRY
• 影响因子: 13.1
• 作者: Delafield, Daniel G.;Lu, Gaoyuan;Li, Lingjun
• 通讯作者: Li, Lingjun

Mass spectrometric based detection of protein nucleotidylation in the RNA polymerase of SARS-CoV-2

基于质谱的 SARS-CoV-2 RNA 聚合酶中蛋白质核苷酸化检测

• DOI: 10.1101/2020.10.07.330324
• 发表时间: 2021
• 期刊: Communications chemistry
• 影响因子: 5.9
• 作者: Conti, Brian J;Leicht, Andrew S;Kirchdoerfer, Robert N;Sussman, Michael R.
• 通讯作者: Sussman, Michael R.

Intermolecular and Intramolecular Interactions of the Arabidopsis Plasma Membrane Proton Pump Revealed Using a Mass Spectrometry Cleavable Cross-Linker

使用质谱可裂解交联剂揭示拟南芥质子泵的分子间和分子内相互作用

• DOI: 10.1021/acs.biochem.0c00268
• 发表时间: 2020
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Nguyen, Thao T.;Blackburn, Matthew R.;Sussman, Michael R.
• 通讯作者: Sussman, Michael R.

Lipo-chitooligosaccharides as regulatory signals of fungal growth and development

• DOI: 10.1038/s41467-020-17615-5
• 发表时间: 2020-08-04
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Rush, Tomas Allen;Puech-Pages, Virginie;Ane, Jean-Michel
• 通讯作者: Ane, Jean-Michel