TRTech-PGR: A mass spectrometric-based interdisciplinary approach to deciphering the molecular dialogue between between crop plants and their microbial friends and foes.

TRTech-PGR：一种基于质谱的跨学科方法，用于破译作物与其微生物朋友和敌人之间的分子对话。

• 批准号: 2010789
• 负责人: Michael Sussman
• 金额: $ 302.7万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-15 至 2024-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2010789&HistoricalAwards=false
• 关键词: TRTech; PGR; mass; spectrometric; based

In soil, the roots of crop plants interact with bacteria and fungi that play interconnected, fundamental roles in agriculture in a process known as ‘symbiosis’ whereby the plants and microbes feed each other with essential nutrients. This interdependent relationship of soil microbes and crop roots provides important elements such as nitrogen and phosphorous to the plant. Without these microbes, farmers must use fertilizers to satisfy the plant’s need for nutrients. Fertilizer use is both financially expensive and environmentally deleterious because the runoff from agricultural fields causes overfertilization of the nation’s waterways. This, in turn, results in disastrous overgrowth of unwanted organisms and large die-offs of desirable organisms like fish and other creatures living in rivers, lakes and oceans. In this project, the essential chemical signals that soil microbes use to tell a plant that they are friends, not foes, so they can live in harmony rather than at war, will be analyzed at a molecular level. In addition, during this project, the proteins in the plant that specifically recognize these signals will be characterized at the molecular level. In general, these signals represent a chemical ‘internet’ in the soil that allows plants and microbes to satisfy their critical roles in modern agriculture. This project will provide a potential strategy for increasing crop productivity needed to nourish a growing population, while decreasing the deleterious financial and environmental costs. In addition, the team will continue to provide an annual course at the University of Wisconsin on advanced methods in the use of mass spectrometry and proteomics.Mass spectrometers play a fundamental role in modern research, as well as in the health and forensics industry. For example, there is a mass spectrometer on Mars looking for signs of life, there is one at the airport that looks for terrorism-related chemicals in our bags and body, and there are mass spectrometers in our hospitals that analyze the presence and metabolism of drugs in our blood and bodily secretions. Despite their importance, most lay citizens and modern research scientists have a poor understanding of what these instruments can do and why. This renewal project is devoted to developing and applying state-of-the-art mass spectrometric technologies to profile early molecular events involved in the recognition of symbiotic microbes with their host crop plants. This project will also assist the general plant community with understanding and using mass spectrometric based analyses of metabolomes and proteomes involved in the plant's ability to recognize and respond to microbes. Finally, via continuation of the annual summer Mass Spectrometry Workshop previously established with prior PGRP NSF funding at UW-Madison, this renewal project will be able to ensure that the mass spectrometric-based technologies being developed will be made available and placed in the hands of the research community's biologists in plant and microbial laboratories throughout the USA.This award was co-funded by the Plant Genome Research Program and the Plant Biotic Interactions Program in the Division of Integrative Organismal Systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在土壤中，作物的根部与细菌和真菌相互作用，在农业中发挥相互联系的基本作用，这一过程被称为“共生”，植物和微生物相互提供必需的营养物质。 土壤微生物和作物根系的这种相互依赖关系为植物提供了重要的元素，如氮和磷。 如果没有这些微生物，农民必须使用肥料来满足植物对营养的需求。 化肥的使用既经济上昂贵又对环境有害，因为农田的径流会导致国家水道的过度施肥。 这反过来又会导致有害生物的灾难性过度生长，以及鱼类和其他生活在河流、湖泊和海洋中的生物的大量死亡。 在这个项目中，土壤微生物用来告诉植物它们是朋友而不是敌人的基本化学信号，因此它们可以和谐相处而不是战争，将在分子水平上进行分析。 此外，在该项目期间，植物中特异性识别这些信号的蛋白质将在分子水平上进行表征。一般来说，这些信号代表了土壤中的化学“互联网”，使植物和微生物能够满足它们在现代农业中的关键作用。该项目将提供一种潜在的战略，以提高作物生产力，满足不断增长的人口的需求，同时降低有害的财务和环境成本。此外，该团队还将继续在威斯康星州大学开设关于质谱和蛋白质组学使用先进方法的年度课程。质谱仪在现代研究以及健康和法医行业中发挥着重要作用。例如，在火星上有一台质谱仪寻找生命迹象，在机场有一台质谱仪在我们的包和身体中寻找与恐怖主义有关的化学物质，在我们的医院里有质谱仪分析我们血液和身体分泌物中药物的存在和代谢。尽管它们很重要，但大多数普通公民和现代研究科学家对这些仪器能做什么以及为什么做的了解很少。该更新项目致力于开发和应用最先进的质谱技术，以分析参与识别共生微生物与其宿主作物植物的早期分子事件。该项目还将帮助一般植物社区了解和使用基于质谱的代谢组和蛋白质组分析，这些代谢组和蛋白质组涉及植物识别和响应微生物的能力。最后，通过延续以前在UW-Madison建立的年度夏季质谱研讨会，这个更新项目将能够确保正在开发的基于质谱的技术将被提供，并置于美国各地植物和微生物实验室的研究界生物学家手中。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Mass spectrometric based detection of protein nucleotidylation in the RNA polymerase of SARS-CoV-2

基于质谱的 SARS-CoV-2 RNA 聚合酶中蛋白质核苷酸化检测

• DOI: 10.1101/2020.10.07.330324
• 发表时间: 2021
• 期刊: Communications chemistry
• 影响因子: 5.9
• 作者: Conti, Brian J;Leicht, Andrew S;Kirchdoerfer, Robert N;Sussman, Michael R.
• 通讯作者: Sussman, Michael R.

Intermolecular and Intramolecular Interactions of the Arabidopsis Plasma Membrane Proton Pump Revealed Using a Mass Spectrometry Cleavable Cross-Linker

使用质谱可裂解交联剂揭示拟南芥质子泵的分子间和分子内相互作用

• DOI: 10.1021/acs.biochem.0c00268
• 发表时间: 2020
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Nguyen, Thao T.;Blackburn, Matthew R.;Sussman, Michael R.
• 通讯作者: Sussman, Michael R.

Lipo-chitooligosaccharides as regulatory signals of fungal growth and development

• DOI: 10.1038/s41467-020-17615-5
• 发表时间: 2020-08-04
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Rush, Tomas Allen;Puech-Pages, Virginie;Ane, Jean-Michel
• 通讯作者: Ane, Jean-Michel

Higher Temperature Porous Graphitic Carbon Separations Differentially Impact Distinct Glycopeptide Classes.

• DOI: 10.1021/jasms.2c00249
• 发表时间: 2023-01-04
• 期刊: JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
• 影响因子: 3.2
• 作者: Delafield, Daniel G.;Miles, Hannah N.;Ricke, William A.;Li, Lingjun
• 通讯作者: Li, Lingjun

Recent Advances in Analytical Approaches for Glycan and Glycopeptide Quantitation.

• DOI: 10.1074/mcp.r120.002095
• 发表时间: 2021
• 期刊: Molecular & cellular proteomics : MCP
• 作者: Delafield DG;Li L
• 通讯作者: Li L