Structure Determination of Photosynthetic Organelles

光合细胞器的结构测定

• 批准号: 1240590
• 负责人: Cheryl Kerfeld
• 金额: $ 29.99万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1240590&HistoricalAwards=false
• 关键词: Structure; Determination; Photosynthetic; Organelles

Intellectual Merit:This project takes advantage of the latest development in X-ray Free Electron Lasers (XFELs) at the Linac Coherent Lisht Source (LCLS) to structurally characterize intact photosynthetic organelles, chlorosomes and carboxysomes. The research undertaking embodies high-risk, high reward research since every experiment undertaken at the LCLS is at the frontier of imaging biology which continues to be under development. Chlorosomes and carboxysomes have not yet been subjected to these methods. Both types of complexes provide unprecedented test cases for XFELs diffraction in that they can both be treated as single particle experiments but with crystalline components. Particles of this size and complexity have not yet been characterized. This is a high risk project in that it is difficult to anticipate the characteristics of the resulting data from these "diffract and destroy" experiments. Methods for processing and analyzing these data will be developed. The results of the project will lay the foundation for visualization of functioning macromolecular organelles. Broader Impact:The broader impact of the project is associated with the training of young scientists at arguably the most exciting intersection of math, physics and biology in a large (9 groups) international collaboration. The pioneering methods developed will be useful for advancing the community of researchers gaining access to LCLS beam-time.This project is jointly funded by the Biomolecular Dynamics Structure and Function Cluster and the Networks and Regulation Cluster.

智力优势：该项目利用直线加速器相干 Lisht 源 (LCLS) 的 X 射线自由电子激光器 (XFEL) 的最新发展，对完整的光合细胞器、叶绿体和羧基体进行结构表征。这项研究工作体现了高风险、高回报的研究，因为 LCLS 进行的每项实验都处于成像生物学的前沿，并且仍在不断发展中。叶色体和羧基体尚未经过这些方法。两种类型的复合体都为 XFEL 衍射提供了前所未有的测试案例，因为它们都可以被视为单粒子实验，但具有晶体成分。这种尺寸和复杂性的颗粒尚未被表征。这是一个高风险项目，因为很难预测这些“衍射和破坏”实验所产生的数据的特征。将开发处理和分析这些数据的方法。该项目的结果将为功能大分子细胞器的可视化奠定基础。更广泛的影响：该项目更广泛的影响与对年轻科学家的培训有关，这可以说是大型（9个小组）国际合作中数学、物理和生物学最令人兴奋的交叉点。所开发的开创性方法将有助于推动研究人员群体获得 LCLS 束时间。该项目由生物分子动力学结构和功能集群以及网络和调节集群共同资助。