Regulatory and Functional Characterization of Modular Photoprotective Proteins in the Context of Cyanobacterial Ecology and Evolution

蓝藻生态和进化背景下模块化光保护蛋白的调控和功能表征

• 批准号: 1557324
• 负责人: Cheryl Kerfeld
• 金额: $ 112.43万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-03-01 至 2021-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1557324&HistoricalAwards=false
• 关键词: Regulatory; Functional; Characterization; Modular; Photoprotective

Photosynthetic organisms convert light energy to chemical energy in the process of photosynthesis. However, absorption of light in excess of that which can be used for photosynthesis can lead to light-induced damage. Thus, photoprotective mechanisms have evolved in photosynthetic organisms to deal with excess light absorption or stressful conditions, including fluctuating light. Photoprotective mechanisms used by cyanobacteria have only recently begun to be characterized. A key factor involved in cyanobacterial photoprotection is the Orange Carotenoid Protein (OCP) that works together with the Fluorescence Recovery Protein (FRP) to convert excess light energy to heat to protect the photosynthetic reaction centers from photodamage, thereby maintaining photosynthetic efficiency and productivity. The proposed work will contribute to a greater understanding of the range of variants of these proteins that are present in ecologically distinct cyanobacteria. In addition to training students and postdoctoral scientists in diverse methods, broader impacts of the research include understanding the mechanisms used by cyanobacteria to protect themselves from excess light absorption and associated light-induced damage. Such knowledge will enhance efforts to increase the efficiency of light capture and reduce photodamage in bioenergy or production strains of cyanobacteria. The project also includes structured mentoring in hands-on research for undergraduates from groups underrepresented in the sciences and participation in an annual Microbiology Day at a local science museum targeting primary and middle school students. In contrast to those of plants, the photoprotective mechanisms of cyanobacteria have only recently begun to be characterized. One of the most prevalent, the OCP/FRP (Orange Carotenoid Protein/Fluorescence Recovery Protein) system responds to high light by dissipating excess energy captured by the light harvesting antenna (phycobilisome or PBS). The OCP is a soluble, 35 kDa protein that binds a single carotenoid molecule. It is the only known photoactive protein that uses a carotenoid as its sole chromophore. The absorption of blue-green light causes the OCP to convert from a dark stable orange form, OCPO, to a light-activated red form, OCPR. OCPR directly participates in photoprotection by binding to the PBS. Recovery from the energy-dissipative state is catalyzed by the FRP. Structurally, the OCP consists of two domains, one serving as a sensor/regulatory domain whereas the other functions directly in energy dissipation. We have identified a family of paralogous genes encoding the separate domains in Fremyella diplosiphon and hypothesize that they reflect the evolution of the OCP and that they provide flexibility in tuning photoprotection in Fremyella under dynamic environmental conditions. A combination of in vivo approaches in Fremyella and in vitro analysis of the isolated proteins will be used in conjunction with bioinformatics to test these hypotheses. In addition to training students and postdoctoral scientists in diverse methods, broader impacts of the research include an in-depth understanding of the flexibility of photoprotective mechanisms that may be useful in bioengineering of cyanobacteria.

光合生物在光合作用过程中将光能转化为化学能。然而，吸收的光超过了可以用于光合作用的光会导致光致损伤。因此，光保护机制已经在光合生物中进化，以处理过度的光吸收或压力条件，包括波动的光。蓝藻使用的光保护机制最近才开始被表征。蓝藻光保护的一个关键因素是橙色类胡萝卜素蛋白（OCP），它与荧光恢复蛋白（FRP）一起将多余的光能转化为热，以保护光合反应中心免受光损伤，从而保持光合效率和生产力。提出的工作将有助于更好地了解这些蛋白质的变异范围，这些蛋白质存在于生态上不同的蓝藻中。除了以各种方法培训学生和博士后科学家外，该研究的更广泛影响还包括了解蓝藻保护自己免受过度光吸收和相关光诱导损伤的机制。这些知识将加强努力，以提高光捕获的效率，减少生物能源或生产菌株的蓝藻的光损伤。该项目还包括为来自科学领域代表性不足的群体的本科生提供实践研究方面的结构化指导，以及参加在当地科学博物馆举办的以中小学生为目标的年度微生物日活动。与植物相比，蓝藻的光保护机制直到最近才开始被表征。其中最流行的是OCP/FRP（橙色类胡萝卜素蛋白/荧光恢复蛋白）系统，该系统通过耗散光收集天线（藻胆异体或PBS）捕获的多余能量来响应强光。OCP是一种可溶性的35kda蛋白质，可以结合单个类胡萝卜素分子。它是唯一已知的光活性蛋白，使用类胡萝卜素作为其唯一的发色团。蓝绿光的吸收使OCP从暗稳定的橙色形式OCPO转变为光激活的红色形式OCPR。OCPR通过与PBS结合直接参与光保护。从能量耗散状态恢复是由FRP催化的。在结构上，OCP由两个结构域组成，一个作为传感器/调节结构域，而另一个直接作用于能量耗散。我们已经确定了一个家族的同源基因编码的不同结构域的水蛭，并假设他们反映了OCP的进化，并提供灵活性调整光保护的水蛭在动态环境条件下。将结合生物信息学，结合体内法和分离蛋白的体外分析来检验这些假设。除了以不同的方法培养学生和博士后科学家外，该研究的更广泛影响包括深入了解可能在蓝藻生物工程中有用的光保护机制的灵活性。