RUI: Proteasomal Removal of Selenoproteins in Plants

RUI：植物中硒蛋白的蛋白酶体去除

• 批准号: 1244009
• 负责人: Douglas Van Hoewyk
• 金额: $ 11.28万
• 依托单位: Coastal Carolina University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-15 至 2017-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1244009&HistoricalAwards=false
• 关键词: RUI; Proteasomal; Removal; Selenoproteins; Plants

Animals (including humans) require selenium to make the amino acid selenocysteine, which is used in the synthesis of essential selenoproteins. In contrast, plants do not require selenium or make specific selenoproteins. Nonetheless, plants can accumulate selenium and play a vital role in providing most of the dietary selenium consumed by humans. However, an excess of selenium leads to toxicity in plants when selenocysteine is incorporated into proteins. Selenoproteins in plants are likely misfolded, and are therefore removed by the ubiquitin-proteasome pathway. The goal of this project is to understand the role of the proteasome and glutathione in removing selenoproteins and alleviating their toxicity in plants. Broader Impacts. The project will directly allow the training of ten undergraduate students, including students with disabilities, at Coastal Carolina University. This project will also facilitate the development of an upper-level laboratory-based class "Plant Adaptations to Stress" which will integrate this research with teaching.

动物（包括人类）需要硒来制造氨基酸硒代半胱氨酸，硒代半胱氨酸用于合成必需的硒蛋白。相比之下，植物不需要硒或产生特定的硒蛋白。 尽管如此，植物可以积累硒，并在提供人类消耗的大部分膳食硒方面发挥重要作用。 然而，当硒代半胱氨酸掺入蛋白质中时，过量的硒导致植物毒性。 植物中的硒蛋白可能是错误折叠的，因此通过泛素-蛋白酶体途径被去除。 本研究的目的是了解蛋白酶体和谷胱甘肽在去除植物中硒蛋白和减轻其毒性中的作用。更广泛的影响。该项目将直接允许在沿海卡罗莱纳大学培训10名本科生，包括残疾学生。该项目还将促进开发一个以实验室为基础的高级课程“植物对压力的适应”，该课程将把这项研究与教学结合起来。