EAGER: RUI: The Physiology and Nitrogen Assimilatory Pathway of Spanish Moss (Tillandsia Usneoides) in Pesponse to Increased Nitrogen
EAGER:RUI:西班牙苔藓(Tillandsia Usneoides)对增加氮的响应的生理学和氮同化途径
基本信息
- 批准号:2040121
- 负责人:
- 金额:$ 15.41万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-01-01 至 2023-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Spanish moss (Tillandsia usneoides) is a slow-growing rootless plant in the Southeastern United States that is often found on trees, such as live oak. Since they don’t have roots in the soil, these “air plants” must acquire nutrients from the atmosphere and from rainfall. Therefore, their environment is extremely nutrient poor compared to other plants. This project investigates how Spanish moss responds to increased amounts of nitrogen, the nutrient that most often restricts plant growth. Nitrogen content in Spanish moss is remarkably lower compared to other plants, which presents a botanical mystery: how does Spanish moss survive in an environment where nitrogen is so scarce? Experiments are designed to determine if Spanish moss is truly nitrogen-limited or rather if these plants have evolved a unique ability to efficiently use limited nitrogen. Understanding how Spanish moss survives despite limited nitrogen could provide insight to plant breeders who seek to improve crop yield on marginalized soils. Additionally, because climate change is predicted to increase atmospheric deposition of nitrogen, these experiments might reveal future growth patterns of Spanish moss. The study includes training of undergraduates from Coastal Carolina University and the National Technical Institute for the Deaf in modern scientific techniques, enhancing their education and the diversity of the future scientific workforce. The central goal of this hypothesis-driven project is to provide evidence if increased nitrogen availability supports enhanced growth of the epiphyte Spanish moss by stimulating the nitrogen assimilation pathway. Biochemical, metabolomic, and physiological approaches will be used to answer two key questions: (i) does Spanish moss display nitrogen luxury consumption and (ii) are these plants nitrogen limited or rather do they display high nitrogen use efficiency? The project will manipulate nitrate and ammonium inputs by both spiking and treating with a physiologically relevant concentrations. The response of the nitrogen assimilation pathway will be assessed by measuring nitrate reductase and glutamine synthetase activity, amino acid and protein concentration, and amino acid biosynthesis. Importantly it will determine if increased flux of inorganic nitrogen into de novo amino acids is coupled with growth. Additionally, experiments will measure the kinetics of nitrate reductase- a rate limiting enzyme in plant nitrogen assimilation- and determine how it is regulated by nitrate and ammonium availability. Spanish moss is tightly tied to southern culture and identity, yet nothing is known about how they metabolize nitrogen. This project will be the first to investigate nitrogen metabolism in these plants, and to determine if increased nitrogen availability and assimilation is coupled to growth.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
铁兰(Tillandsia usneoides)是一种生长缓慢的无根植物,生长在美国东南部,通常生长在橡树等树木上。 由于它们在土壤中没有根,这些“空气植物”必须从大气和降雨中获得营养。 因此,与其他植物相比,它们的生长环境极其贫瘠。 该项目调查了西班牙苔藓如何应对氮的增加,氮是最经常限制植物生长的营养素。 与其他植物相比,西班牙苔藓中的氮含量明显较低,这提出了一个植物学之谜:西班牙苔藓如何在氮如此稀缺的环境中生存? 实验旨在确定西班牙苔藓是否真的是氮有限的,或者这些植物是否已经进化出一种独特的能力来有效地利用有限的氮。 了解西班牙苔藓如何在氮有限的情况下生存,可以为寻求在边缘化土壤上提高作物产量的植物育种者提供见解。 此外,由于气候变化预计会增加大气中氮的沉积,这些实验可能会揭示西班牙苔藓未来的生长模式。 这项研究包括对来自沿海卡罗莱纳大学和国家聋人技术研究所的本科生进行现代科学技术培训,加强他们的教育和未来科学劳动力的多样性。 这个假设驱动的项目的中心目标是提供证据,如果增加氮的可用性支持增强的附生植物西班牙藓的生长,通过刺激氮同化途径。生物化学,代谢组学和生理学的方法将被用来回答两个关键问题:(一)没有西班牙藓显示氮奢侈消费和(ii)这些植物氮有限,或者更确切地说,他们显示高氮利用效率? 该项目将通过添加和处理生理相关浓度来控制硝酸盐和铵的投入。 氮同化途径的响应将通过测量硝酸还原酶和谷氨酰胺合成酶活性、氨基酸和蛋白质浓度以及氨基酸生物合成来评估。 重要的是,它将确定无机氮进入从头氨基酸的增加的通量是否与生长相关联。 此外,实验将测量硝酸还原酶的动力学-植物氮同化的限速酶-并确定它是如何通过硝酸盐和铵的可用性调节。 西班牙苔藓与南方的文化和身份紧密相连,但对它们如何代谢氮却一无所知。 该项目将是第一个调查这些植物氮代谢的项目,并确定增加的氮可用性和同化作用是否与生长有关。该奖项反映了NSF的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(0)
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Douglas Van Hoewyk其他文献
Endomycorrhizal colonization of Dasiphora floribunda, a native plant species of calcareous wetlands in eastern New York State, USA
- DOI:
10.1672/0277-5212(2001)021[0431:ecodfa]2.0.co;2 - 发表时间:
2001-09-01 - 期刊:
- 影响因子:2.000
- 作者:
Douglas Van Hoewyk;Cathleen Wigand;Peter M. Groffman - 通讯作者:
Peter M. Groffman
Douglas Van Hoewyk的其他文献
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{{ truncateString('Douglas Van Hoewyk', 18)}}的其他基金
RUI: reprogramming cellular processes in proteasome-inhibited plants
RUI:在蛋白酶体抑制的植物中重新编程细胞过程
- 批准号:
1615318 - 财政年份:2016
- 资助金额:
$ 15.41万 - 项目类别:
Standard Grant
RUI: Proteasomal Removal of Selenoproteins in Plants
RUI:植物中硒蛋白的蛋白酶体去除
- 批准号:
1244009 - 财政年份:2013
- 资助金额:
$ 15.41万 - 项目类别:
Standard Grant
Collaborative Research: Regulation of CpNifS/CpSufE1-Mediated Iron-Sulfur Cluster Synthesis in Plant Plastids. Implications for Sulfur and Iron Metabolism and Selenium Tolerance
合作研究:植物质体中 CpNifS/CpSufE1 介导的铁硫簇合成的调控。
- 批准号:
0950648 - 财政年份:2010
- 资助金额:
$ 15.41万 - 项目类别:
Continuing Grant
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