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RUI: reprogramming cellular processes in proteasome-inhibited plants

RUI：在蛋白酶体抑制的植物中重新编程细胞过程

基本信息

批准号：
1615318
负责人：
Douglas Van Hoewyk
金额：
$ 18.46万
依托单位：
Coastal Carolina University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2016
资助国家：
美国
起止时间：
2016-08-01 至 2021-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1615318&HistoricalAwards=false
关键词：
RUI reprogramming cellular processes proteasome

项目摘要

The goal of this project is to better understand how plants respond to environmental stress through the study of proteasomes and proteasome inhibition. Proteasomes are protein complexes inside all plant and animal cells that degrade damaged proteins to prevent their toxic aggregation. Such damage can arise from physical stress; however, such stressors may also inhibit proteasome activity. Thus, the focus of the research will be to understand how plants reprogram their cellular activities during proteasome inhibition. A better understanding of plant responses to environmental stress may ultimately improve crop yield and food security. The project will allow the training of twelve undergraduate students at Coastal Carolina University. Students with physical disabilities will participate in the research, and will strengthen the ties between the College of Science and Office of Accessibility and Disability Services; this collaboration may serve as a model to other universities seeking to increase this group?s participation in STEM disciplines. As this project integrates both research and teaching, it will also facilitate the revision of a laboratory-based class that will serve to strengthen the curriculum. This research investigates plant proteasomes, which avert cytotoxicity by safely removing damaged proteins that result from stress. However, severe oxidative stress decreases proteasome activity in plants, and this project aims to identify mechanisms that contribute to its impairment. In contrast to yeast and mammalian models, the effects of proteasome inhibition in plants are not understood. This project intends to address this gap in plant biology by examining how cellular processes are altered in response to proteasome inhibition. Respirometry, biochemical, and -omic approaches will be used to reveal if proteasome inhibition coordinates metabolism to restore amino acid levels or adjust antioxidant metabolism. The outcomes of the study will describe differences in how plants respond to proteasome inhibition compared to mammalian and yeast cells.

该项目的目的是通过研究蛋白酶体和蛋白酶体抑制来更好地了解植物对环境胁迫的反应。蛋白酶体是存在于所有植物和动物细胞中的蛋白质复合物，它能降解受损的蛋白质以防止它们的毒性聚集。这种损伤可能是由身体压力引起的；然而，这些应激源也可能抑制蛋白酶体的活性。因此，研究的重点将是了解植物在蛋白酶体抑制过程中如何重新编程其细胞活动。更好地了解植物对环境胁迫的反应可能最终提高作物产量和粮食安全。该项目将允许在卡罗莱纳海岸大学培训12名本科生。有身体残疾的学生将参与研究，并将加强理学院与无障碍和残疾服务办公室之间的联系；这种合作可能会成为其他大学寻求增加这一群体的榜样。美国对STEM学科的参与。由于这个项目整合了研究和教学，它也将促进以实验室为基础的课程的修订，这将有助于加强课程。本研究研究了植物蛋白酶体，它通过安全地去除因压力而受损的蛋白质来避免细胞毒性。然而，严重的氧化应激会降低植物的蛋白酶体活性，本项目旨在确定导致其损伤的机制。与酵母和哺乳动物模型相反，植物中蛋白酶体抑制的作用尚不清楚。该项目旨在通过研究细胞过程如何改变以响应蛋白酶体抑制来解决植物生物学中的这一空白。呼吸测量、生化和组学方法将用于揭示蛋白酶体抑制是否协调代谢以恢复氨基酸水平或调节抗氧化代谢。该研究的结果将描述与哺乳动物和酵母细胞相比，植物对蛋白酶体抑制的反应方式的差异。