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EAGER: Comparison of Amino-Acid and Bulk-Tissue Stable Isotope Techniques for Analyzing Modern and Historical Food Webs

EAGER：分析现代和历史食物网的氨基酸和整体组织稳定同位素技术的比较

基本信息

批准号：
1249370
负责人：
James Thorp
金额：
$ 10万
依托单位：
University of Kansas Center for Research Inc
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-15 至 2014-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1249370&HistoricalAwards=false
关键词：
EAGER Comparison Amino Acid Bulk

项目摘要

Food web structure is an important indicator of ecosystem health because it reflects both species richness and functional diversity. Recent attempts to use compound-specific stable isotopes rather than bulk-tissue techniques to evaluate food webs promise to improve understanding of food web dynamics and trophic interactions. These new methods, which rely on amino acid isotope analyses, need to be validated experimentally before this promising advance can be applied to natural food webs. This study relies on a laboratory multi-level food chain experiment to compare the ecological sensitivity and the costs and benefits of new amino acid techniques and common bulk-tissue methods. The researchers will create a realistic, four-level food chain in the laboratory that allows consumers to integrate prey isotopic signatures into their tissues. At the end of the experiment, both amino acid and bulk tissue stable isotopes will be analyzed to determine whether amino acids provide a more accurate measure of trophic position than bulk tissue methods.The broader impacts of this project lie primarily in advancing a new method for evaluating natural food webs. If successful, the method could eventually be applied to terrestrial and aquatic communities, and would advance the general field of food web ecology. It also could be applied to museum specimens, allowing researchers to examine historical as well as contemporary food webs.

食物网结构是生态系统健康的一个重要指标，因为它反映了物种丰富度和功能多样性。最近尝试使用化合物特定的稳定同位素，而不是散装组织技术来评估食物网的承诺，以提高对食物网动力学和营养相互作用的理解。这些依赖于氨基酸同位素分析的新方法，需要在这一有前途的进展应用于自然食物网之前进行实验验证。本研究以实验室多层次食物链实验为基础，比较了氨基酸新技术和普通组织块法的生态敏感性和成本效益。研究人员将在实验室中创建一个现实的四级食物链，允许消费者将猎物的同位素特征整合到他们的组织中。在实验结束时，将分析氨基酸和大量组织稳定同位素，以确定氨基酸是否提供比大量组织方法更准确的营养位置测量，该项目的更广泛的影响主要在于提出一种评估自然食物网的新方法。如果成功，该方法最终可以应用于陆地和水生群落，并将推进食物网生态学的一般领域。它也可以应用于博物馆标本，使研究人员能够检查历史以及当代的食物网。