CAREER: Genome-wide Approaches to Elucidating the Role of Nonsense-mediated mRNA Decay in Regulating Gene Expression

职业：利用全基因组方法阐明无义介导的 mRNA 衰变在调节基因表达中的作用

• 批准号: 1253788
• 负责人: Kristian Baker
• 金额: $ 130.72万
• 依托单位: Case Western Reserve University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2020-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1253788&HistoricalAwards=false
• 关键词: CAREER; Genome; wide; Approaches; Elucidating

Intellectual Merit: Cells have evolved elaborate mechanisms to ensure faithful expression of the genetic material. In cases where DNA copies harbor mutations that lead to expression of incomplete proteins, a quality control strategy distinguishes and rapidly eliminates the aberrant material from the cell. How the cell provides this critical safeguard while maintaining accurate gene expression remains an important unanswered biological question. Using a comprehensive set of emerging scientific technologies, this research will identify and characterize the targets of this quality control pathway in an effort to better understand how this level of protective gene regulation is achieved.Broader Impacts: The research objectives outlined in this project provide an ideal framework to educate, train, and mentor high school, undergraduate and graduate students in critical thinking, scientific method, and hands-on experimental approaches. The development of research skills by junior scientists in the laboratory will be coupled with the launch of an undergraduate course directed at preparing women and minorities pursuing careers in science, engineering and mathematics for academic and institutional challenges they might face and resources available to help maximize success in these fields. Together, these classroom and laboratory opportunities will strengthen the learning and enhance the competitiveness of young scientists at various stages of their training.

智力优势：细胞已经进化出复杂的机制来确保遗传物质的忠实表达。在DNA拷贝携带突变导致不完整蛋白质表达的情况下，一种质量控制策略可以区分并迅速消除细胞中的异常物质。细胞如何提供这种关键的保护，同时保持准确的基因表达仍然是一个重要的未解生物学问题。利用一套全面的新兴科学技术，本研究将识别和表征这一质量控制途径的目标，以更好地理解这种水平的保护性基因调控是如何实现的。更广泛的影响：本项目概述的研究目标提供了一个理想的框架，以教育、培训和指导高中生、本科生和研究生批判性思维、科学方法和实践实验方法。在实验室培养初级科学家的研究技能的同时，还将开设一门本科课程，旨在为追求科学、工程和数学职业的妇女和少数民族做好准备，以应对她们可能面临的学术和体制挑战，并利用现有资源帮助她们在这些领域取得最大成功。这些课堂和实验室的机会将加强年轻科学家在不同训练阶段的学习和提高他们的竞争力。