Biomechanicsof Self-Assembly of the Lepidopteran Feeding Device

鳞翅目摄食装置自组装的生物力学

• 批准号: 1305338
• 负责人: Konstantin Kornev
• 金额: $ 39.81万
• 依托单位: Clemson University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-01 至 2017-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1305338&HistoricalAwards=false
• 关键词: Biomechanicsof; Self; Assembly; Lepidopteran; Feeding

The overarching goal of this project is to understand the biomechanical principles of proboscis self-assembly and to apply these principles to engineer fiber-based self- assembling microfluidic devices for manipulation of micro and nano droplets. The model system under investigation is the proboscis feeding tube of butterflies. This tube is assembled and hardened after emergence of the adult from the pupal encasement by joining dorsal and ventral galeae fibers and cuticle hardening. The mature proboscis is coiled but can be fully extended during the feeding process; the extension involves antiparallel sliding of the galeae fibers. The goals of the project are (i) to study the effect of saliva in creating capillary pressure that forces the two galeae together (the elasto-capillary effect) in live butterflies and a model system of fiber rails, (ii) to study saliva behavior during this process of proboscis assembly, using X-ray phase-contrast imaging, (iii) to provide full characterization of mechanical and wetting properties of the proboscis to evaluate the model parameters and assess the applicability of different self-assembling and hardening scenarios, and (iv) to develop a fiber-rail type microfluidic system that that relies on capillary action and would work on the principles of proboscis assembly/disassembly.

本项目的总体目标是了解长鼻自组装的生物力学原理，并将这些原理应用于设计基于纤维的自组装微流体装置，用于操纵微纳米液滴。所研究的模型系统是蝴蝶的喙部喂食管。成虫出蛹后，通过连接背侧和腹侧的盔瓣纤维和角质层硬化，形成管状结构并硬化。成熟的喙是盘绕的，但在进食过程中可以完全伸展；延伸涉及到galgalae纤维的反平行滑动。该项目的目标是(i)在活蝴蝶和纤维轨道模型系统中研究唾液在产生毛细压力时的作用，这种毛细压力迫使两个galeae在一起（弹性毛细效应）；（ii）使用x射线相对比成像研究唾液在长鼻组装过程中的行为；（iii）提供完整的机械和润湿特性表征，以评估模型参数，并评估不同自组装和硬化场景的适用性；（iv）开发一种依赖于毛细作用的纤维导轨型微流体系统，该系统将基于喙的组装/拆卸原理工作。