A "Gold" Standard for Understanding Protein-Nanoparticle Interactions

理解蛋白质-纳米颗粒相互作用的“黄金”标准

• 批准号: 1306596
• 负责人: Catherine Murphy
• 金额: $ 43.5万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1306596&HistoricalAwards=false
• 关键词: Gold; Standard; Understanding; Protein; Nanoparticle

The Macromolecular, Supramolecular and Nanochemistry Program of the Division of Chemistry supports Professor Catherine J. Murphy of the University of Illinois at Urbana-Champaign on a project focusing on the nanoparticle-biology interface. Gold nanoparticles of various sizes and shapes are being developed as "gold standards" for the field. The thermodynamics, kinetics, geometry and cooperativity of protein-nanoparticle binding are revealed by numerous spectroscopic and mass spectrometric methods. The major hypothesis to be tested is that the underlying surface chemistry of the nanoparticles dictates the final biological behavior of the entire ensemble. LeChatelier's Principle, familiar from general chemistry, is being tested on the nanoscale in the context of gold nanoparticle-protein interactions, since light absorption by the nanoparticles leads to heating, which in turn can lead to either stronger or weaker binding depending on the enthalpies of the interactions.This fundamental work at the interface of nanoparticles and biological molecules is to enable future applications and best practices in the area of nanobiotechnology. The applications include photothermal destruction of pathogenic or cancerous cells; improved drug delivery vehicles based on nanoparticles; and improved sensors for molecular diagnostics. Graduate students and undergraduate students trained in the laboratory gain great transdisciplinary experience at the frontiers of science, including opportunities to explain their science to nonscientists. The PI is developing educational modules about nanotechnology and interfacial chemistry for massive open online courses, freely available to the world.

化学系的大分子，超分子和纳米化学计划支持伊利诺伊大学厄巴纳-香槟分校的凯瑟琳·墨菲教授开展一个专注于纳米粒子生物学界面的项目。 各种尺寸和形状的金纳米颗粒正在被开发为该领域的“黄金标准”。 蛋白质-纳米颗粒结合的热力学、动力学、几何形状和协同性通过多种光谱和质谱方法揭示。 待检验的主要假设是纳米颗粒的潜在表面化学决定了整个集合体的最终生物学行为。 从普通化学中熟悉的勒查特列原理，正在纳米级的金纳米颗粒-蛋白质相互作用的背景下进行测试，因为纳米颗粒的光吸收导致加热，这反过来又可以导致更强或更弱的结合，这取决于相互作用的性质。在纳米颗粒和生物分子的界面上的这项基础工作是使未来的应用和最佳实践成为可能。纳米生物技术领域。 这些应用包括病原体或癌细胞的光热破坏;基于纳米颗粒的改进的药物递送载体;以及改进的分子诊断传感器。在实验室接受培训的研究生和本科生在科学前沿获得了丰富的跨学科经验，包括向非科学家解释他们的科学的机会。 PI正在开发有关纳米技术和界面化学的教育模块，用于大规模开放式在线课程，免费向世界提供。