Conformational Proteomics on Nanoparticle Surfaces

在这项由化学系大分子、超分子和纳米科学计划资助的项目中，伊利诺伊大学香槟分校的凯瑟琳·墨菲教授研究了纳米颗粒表面蛋白质的构象(三维形状)作为纳米颗粒大小、形状和表面化学的函数。这些实验的洞察力可能会改善对分子和潜在细胞水平上的生物效应的预测。尺寸在10-100 nm范围内的金属纳米颗粒表现出不同寻常的性质，使其能够在生物学中应用，从诊断到治疗。这些纳米颗粒表面的化学细节很重要。一些表面化学物质促进蛋白质结合。有些则不鼓励蛋白质结合，有些甚至控制蛋白质与表面结合的方式。蛋白质等分子在纳米粒子表面的位置会影响这些纳米粒子在生物系统中的命运和功能。该项目还有助于在纳米技术、生物纳米技术和分析测量领域对本科生和研究生进行教育和培训。这种培训提供了一批全面发展的科学家，他们为创新经济做出了贡献。创建并广泛传播关于表面化学的课堂模块，以使学生能够了解这些科学主题。面向非理科学生的宣传活动有助于教育非科学家有关生物纳米技术的知识，并提高理科学生的沟通技能。一系列广泛的实验被设计用来研究“纳米颗粒上的构象蛋白质组学”现象。该研究小组正在开发测量蛋白质构象，然后合理改变蛋白质在纳米颗粒表面的构象/取向的能力。这些系统包括带有各种有机配位体外壳的金核或新的多孔、约束性外壳。这些蛋白质包括一组精心挑选的小分子，具有本质上有趣的生物活性。使用的方法包括对蛋白质行为的自适应控制，这种控制来自表面的“等离子刷新”，或者在靠近金属表面的设计好的孔中捕获蛋白质构象。结合蛋白质构象和取向的测量来自于核磁共振方法、足迹质谱学和振动光谱学的组合。

In this project funded by the Macromolecular, Supramolecular, and Nanoscience program of the Chemistry Division, Professor Catherine Murphy of the University of Illinois at Urbana-Champaign addresses the conformation (three-dimensional shape) of proteins on nanoparticle surfaces as a function of nanoparticle size, shape, and surface chemistry. Insights from these experiments may lead to improved prediction of biological effects at the molecular and potentially cellular levels. Metallic nanoparticles in the 10-100 nm size range exhibit unusual properties that enable applications in biology, ranging from diagnostics to therapeutics. The details of the chemistry at the surface of these nanoparticles matter. Some surface chemistries promote protein binding. Others discourage protein binding, and some even control how proteins bind to the surface. The position of molecules such as proteins on the surface of nanoparticles can influence the fate and function of these nanoparticles in biological systems. The project also contributes to the education and training of undergraduate and graduate students in the areas of nanotechnology, bionanotechnology, and analytical measurements. This training provides a pipeline of well-rounded scientists who contribute to the innovation economy. Classroom modules on surface chemistry are created and broadly disseminated to enable students to learn about these scientific topics. Outreach to non-science students helps educate non-scientists about bionanotechnology as well as improve the communication skills of the science students.A wide-ranging set of experiments are designed to examine the phenomenon of "conformational proteomics on nanoparticles." This research group is developing the ability to measure protein conformations and then rationally alter the conformation/orientation of proteins on nanoparticle surfaces. The systems include gold cores with various organic ligand shells or new porous, constraining shells. The proteins include a small, carefully selected set of molecules with intrinsically interesting bioactivity. The methods used include adaptive control of protein behavior that comes from "plasmonic refreshing" of the surface or capturing protein conformers in designed pores near the metal surface. The measurement of bound protein conformation and orientation comes from a combination of NMR methods, footprinting mass spectrometry, and vibrational spectroscopy.