Conformational Proteomics on Nanoparticle Surfaces

纳米颗粒表面的构象蛋白质组学

• 批准号: 1608743
• 负责人: Catherine Murphy
• 金额: $ 61万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-15 至 2020-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1608743&HistoricalAwards=false
• 关键词: Conformational; Proteomics; Nanoparticle; Surfaces

In this project funded by the Macromolecular, Supramolecular, and Nanoscience program of the Chemistry Division, Professor Catherine Murphy of the University of Illinois at Urbana-Champaign addresses the conformation (three-dimensional shape) of proteins on nanoparticle surfaces as a function of nanoparticle size, shape, and surface chemistry. Insights from these experiments may lead to improved prediction of biological effects at the molecular and potentially cellular levels. Metallic nanoparticles in the 10-100 nm size range exhibit unusual properties that enable applications in biology, ranging from diagnostics to therapeutics. The details of the chemistry at the surface of these nanoparticles matter. Some surface chemistries promote protein binding. Others discourage protein binding, and some even control how proteins bind to the surface. The position of molecules such as proteins on the surface of nanoparticles can influence the fate and function of these nanoparticles in biological systems. The project also contributes to the education and training of undergraduate and graduate students in the areas of nanotechnology, bionanotechnology, and analytical measurements. This training provides a pipeline of well-rounded scientists who contribute to the innovation economy. Classroom modules on surface chemistry are created and broadly disseminated to enable students to learn about these scientific topics. Outreach to non-science students helps educate non-scientists about bionanotechnology as well as improve the communication skills of the science students.A wide-ranging set of experiments are designed to examine the phenomenon of "conformational proteomics on nanoparticles." This research group is developing the ability to measure protein conformations and then rationally alter the conformation/orientation of proteins on nanoparticle surfaces. The systems include gold cores with various organic ligand shells or new porous, constraining shells. The proteins include a small, carefully selected set of molecules with intrinsically interesting bioactivity. The methods used include adaptive control of protein behavior that comes from "plasmonic refreshing" of the surface or capturing protein conformers in designed pores near the metal surface. The measurement of bound protein conformation and orientation comes from a combination of NMR methods, footprinting mass spectrometry, and vibrational spectroscopy.

在这个由化学学部大分子、超分子和纳米科学项目资助的项目中，伊利诺伊大学厄巴纳-香槟分校的凯瑟琳·墨菲教授研究了纳米颗粒表面蛋白质的构象（三维形状），这是纳米颗粒大小、形状和表面化学的函数。从这些实验中获得的见解可能会导致在分子和潜在的细胞水平上改进生物效应的预测。10-100纳米尺寸范围内的金属纳米颗粒表现出不同寻常的特性，使其能够应用于从诊断到治疗的生物学领域。这些纳米颗粒表面的化学细节很重要。一些表面化学物质促进蛋白质结合。另一些则阻碍蛋白质结合，有些甚至控制蛋白质如何与表面结合。蛋白质等分子在纳米颗粒表面的位置可以影响这些纳米颗粒在生物系统中的命运和功能。该项目还有助于纳米技术、生物纳米技术和分析测量领域的本科生和研究生的教育和培训。这种培训提供了为创新经济做出贡献的全面发展的科学家的管道。创建并广泛传播表面化学课堂模块，使学生能够学习这些科学主题。与非理科生的接触有助于对非科学家进行生物纳米技术的教育，并提高理科生的沟通技巧。一系列广泛的实验被设计用来检验“纳米颗粒上的构象蛋白质组学”现象。这个研究小组正在开发测量蛋白质构象的能力，然后合理地改变纳米颗粒表面蛋白质的构象/方向。该系统包括具有各种有机配体壳或新的多孔、约束壳的金核。这些蛋白质包括一组精心挑选的小分子，它们具有内在有趣的生物活性。所使用的方法包括自适应控制蛋白质行为，这种行为来自表面的“等离子体刷新”或在金属表面附近的设计孔中捕获蛋白质构象。结合蛋白构象和取向的测量来自核磁共振方法，足迹质谱和振动光谱的组合。