Instrumentation Development: Label-free and rapid 3D-nanostructure ultrathin-layer birefringence imaging chromatography

仪器开发：无标记快速 3D 纳米结构超薄层双折射成像色谱

• 批准号: 1309806
• 负责人: David Hage
• 金额: $ 40.25万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2017-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1309806&HistoricalAwards=false
• 关键词: Instrumentation; Development; Label; free; rapid

With support from the Division of Chemistry - Chemical Measurement and Imaging Program, David Hage from the Department of Chemistry and Tino Hofmann from the Department of Electrical Engineering and their students at the University of Nebraska-Lincoln will develop instrumentation for label-free and rapid detection in ultrathin-layer imaging chromatography by using highly-oriented 3D nanostructure ultrathin-layer supports in combination with transmission birefringence imaging detection schemes. This approach, which is based on ellipsometric measurements, will use the detection of spatial and time-resolved microscopic images of linear birefringence variations in nanoengineered supports upon the interactions of these supports with analytes.Chromatography methods analyze mixtures by separating them into their components. Ultrathin-layer liquid chromatography (UTLC) methods separate components using their interactions with very thin, specially engineered (nanostructured) films. Often these methods require that the mixture components be labeled or tagged so that they can be detected. This work is expected to reduce the limit of detection of UTLC by two orders of magnitude when compared to current state-of-the-art label-free UTLC systems; this will be a groundbreaking advancement in this technology. The application of this separation and imaging system, which will have essentially universal applicability to small chemical targets, will benefit chemical analysis in many fields, including biochemical, environmental, and pharmaceutical testing. In addition to the significant advantages of the new measurement scheme compared to current UTLC systems, the increased sensitivity to minute amounts of chemicals that are adsorbed to nanostructured surfaces will also provide crucial information about the separation and flow-based properties of the nanostructured surfaces. This project provides a unique opportunity for students to participate in the development of new instrumentation and interdisciplinary research.

在化学划分的支持下 - 化学测量和成像计划，内布拉斯加州林肯大学电气工程系及其学生的化学和蒂诺·霍夫曼（Tino Hofmann方案。这种方法基于椭圆测量，将使用这些支持与分析的相互作用的纳米工程支持中线性双折射变化的空间和时间分辨显微镜图像的检测。染料方法与分析的相互作用。染料方法通过将它们分离成各个组件来分析混合物。 超薄层液相色谱法（UTLC）方法使用它们的相互作用与非常薄的特殊设计（纳米结构化）膜分开组件。 通常，这些方法要求将混合物组件标记或标记，以便可以检测到它们。 与当前无标签的UTLC系统相比，这项工作有望将UTLC的检测限量降低两个数量级。这将是这项技术的开创性进步。这种分离和成像系统的应用将基本上具有对小化学靶标的普遍适用性，将使许多领域的化学分析受益，包括生化，环境和药物测试。除了与当前UTLC系统相比，新测量方案的显着优势外，对纳米结构表面吸附的微量化学物质的敏感性提高还将提供有关纳米结构表面的基于基于流动性的特性的重要信息。 该项目为学生提供了一个独特的机会，可以参与新的仪器和跨学科研究的发展。