MRI: Track 1 Acquisition of a SEC/FFF-MALS-DLS

该奖项由主要研究仪器、刺激竞争性研究的既定计划（EPSCoR）和化学研究仪器计划共同支持。内布拉斯加州大学林肯分校正在获得一个仪器平台，该平台将化学分离方法(粒径排除色谱法（SEC）和场流分选（FFF）与多角度光散射和动态光散射（MALS-DLS）检测方法相结合，以支持David S. Hage教授、Chin Li （Barry）张、Edward Harris、Wei Niu和Angela K. Pannier教授的研究。该仪器促进了该机构化学，生物化学，化学和生物分子工程以及生物系统工程领域的研究。SEC根据从填充柱中洗脱出的分子大小来分离分子。FFF是一种多功能的分离技术，可以应用于大分子和纳米颗粒。MALS-DLS随后被用于在线测量散射光的随时间波动，这与分子扩散系数及其大小分布有关。该仪器提高了许多院系各级学生的教育、研究和教学努力，并为附近的机构提供了可访问性。该仪器被用于研究培训机会，影响从纳米材料和聚合物到生物医学研究和环境研究的许多领域。学生，当地工业和其他人通过将仪器整合到各种程序中获得实际操作经验。SEC/FFF-MALS-DLS的授予旨在加强各级的研究和教育，特别是在化学、聚合物科学、生物化学、化学和生物分子工程以及生物系统工程等领域。研究重点是环境和化学研究，旨在检查药物在水中的活性及其与溶解有机物的结合，以及表征纳米塑料和微塑料在水中降解的新方法。该仪器还用于分离复杂碳水化合物透明质酸的特定尺寸形式，以检查其生物活性，表征和分离重组蛋白及其结构域，用于酶工程，并分离和表征用于生物医学研究的合成纳米颗粒和生物囊泡。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

This award is jointly supported by the Major Research Instrumentation, the Established Program to Stimulate Competitive Research (EPSCoR), and the Chemistry Research Instrumentation Programs. The University of Nebraska – Lincoln is acquiring an instrumental platform that combines the chemical separation methods of size-exclusion chromatography (SEC) and field-flow fractionation (FFF) with the detection methods of multi-angle light scattering and dynamic light scattering (MALS-DLS) to support the research of Professor David S. Hage, Chin Li (Barry) Cheung, Edward Harris, Wei Niu, and Angela K. Pannier. This instrument facilitates research in the areas of chemistry, biochemistry, chemical and biomolecular engineering, and biological systems engineering at this institution. SEC separates molecules as a function of size based on their elution from a packed column. FFF is a versatile separation technique that can be applied to macromolecules and nanoparticles. MALS-DLS are subsequently used, in-line, to measure time-dependent fluctuations in scattered light, which is related to molecular diffusion coefficients and their size distributions. This instrument enhances the educational, research, and teaching efforts of students at all levels in many departments as well as provides accessibility for use at nearby institutions. The instrument is leveraged in research training opportunities that impacts many areas spanning from nanomaterials and polymers to biomedical research and environmental studies. Students, local industry and others gain hands-on experience with the instrument through its integration in various program.The award of the SEC/FFF-MALS-DLS is aimed at enhancing research and education at all levels, especially in areas such as chemistry, polymer science, biochemistry, chemical and biomolecular engineering, and biological systems engineering. Research focuses on environmental and chemical research aimed at examining the activity of pharmaceuticals in water and their binding with dissolved organic matter, as well as the characterization of new methods for the degradation of nanoplastics and microplastics in water. The instrument is also used to isolate size-specific forms of the complex carbohydrate hyaluronan for examining their biological activities, to characterize and separate recombinant proteins and their domains for enzyme engineering, and to isolate and characterize synthetic nanoparticles and biological vesicles for use in biomedical research.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.