MRI: Track 1 Acquisition of a SEC/FFF-MALS-DLS

MRI： SEC/FFF-MALS-DLS 的轨道 1 采集

• 批准号: 2320239
• 负责人: David Hage
• 金额: $ 47.81万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2320239&HistoricalAwards=false
• 关键词: MRI; Track; Acquisition; SEC; FFF

This award is jointly supported by the Major Research Instrumentation, the Established Program to Stimulate Competitive Research (EPSCoR), and the Chemistry Research Instrumentation Programs. The University of Nebraska – Lincoln is acquiring an instrumental platform that combines the chemical separation methods of size-exclusion chromatography (SEC) and field-flow fractionation (FFF) with the detection methods of multi-angle light scattering and dynamic light scattering (MALS-DLS) to support the research of Professor David S. Hage, Chin Li (Barry) Cheung, Edward Harris, Wei Niu, and Angela K. Pannier. This instrument facilitates research in the areas of chemistry, biochemistry, chemical and biomolecular engineering, and biological systems engineering at this institution. SEC separates molecules as a function of size based on their elution from a packed column. FFF is a versatile separation technique that can be applied to macromolecules and nanoparticles. MALS-DLS are subsequently used, in-line, to measure time-dependent fluctuations in scattered light, which is related to molecular diffusion coefficients and their size distributions. This instrument enhances the educational, research, and teaching efforts of students at all levels in many departments as well as provides accessibility for use at nearby institutions. The instrument is leveraged in research training opportunities that impacts many areas spanning from nanomaterials and polymers to biomedical research and environmental studies. Students, local industry and others gain hands-on experience with the instrument through its integration in various program.The award of the SEC/FFF-MALS-DLS is aimed at enhancing research and education at all levels, especially in areas such as chemistry, polymer science, biochemistry, chemical and biomolecular engineering, and biological systems engineering. Research focuses on environmental and chemical research aimed at examining the activity of pharmaceuticals in water and their binding with dissolved organic matter, as well as the characterization of new methods for the degradation of nanoplastics and microplastics in water. The instrument is also used to isolate size-specific forms of the complex carbohydrate hyaluronan for examining their biological activities, to characterize and separate recombinant proteins and their domains for enzyme engineering, and to isolate and characterize synthetic nanoparticles and biological vesicles for use in biomedical research.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项由重大研究仪器、既定的激励竞争研究计划(EPSCoR)和化学研究仪器计划共同支持。内布拉斯加州大学林肯分校正在获取一个仪器平台，该平台将尺寸排除层析(SEC)和场流分离(FFF)的化学分离方法与多角度光散射和动态光散射(MALS-DLS)的检测方法相结合，以支持David S.Hage教授、Chin Li(Barry)Cheung、Edward Harris、魏牛和Angela K.Pannier的研究。该仪器促进了该机构在化学、生物化学、化学和生物分子工程以及生物系统工程领域的研究。SEC根据分子从填充柱中洗脱出来，根据分子大小进行分离。FFF是一种多功能的分离技术，可以应用于大分子和纳米颗粒。随后，MALS-DLS被用于在线测量散射光中随时间变化的波动，这与分子扩散系数及其尺寸分布有关。这一工具加强了许多部门各级学生的教育、研究和教学努力，并提供了在附近机构使用的便利条件。该仪器被用于研究培训机会，影响从纳米材料和聚合物到生物医学研究和环境研究的许多领域。学生、本地工业界及其他人士透过将仪器整合在不同的项目中，获得实际操作经验。SEC/FFF-MALS-DLS奖项旨在加强各层次的研究及教育，特别是在化学、聚合物科学、生物化学、化学及生物分子工程及生物系统工程等领域。研究的重点是环境和化学研究，目的是检查药物在水中的活性及其与溶解有机物质的结合，以及表征降解水中纳米塑料和微塑料的新方法。该仪器还用于分离特定大小的复合碳水化合物透明质酸，用于检查它们的生物活性，表征和分离重组蛋白质及其结构域，用于酶工程，以及分离和表征用于生物医学研究的合成纳米颗粒和生物囊泡。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。