MRI: Track 1 Acquisition of a SEC/FFF-MALS-DLS

该奖项由主要研究仪器，刺激竞争性研究的既定计划（EPSCoR）和化学研究仪器计划共同支持。内布拉斯加大学林肯分校正在收购一个仪器平台，该平台将尺寸排阻色谱（SEC）和场流分馏（FFF）的化学分离方法与多角度光散射和动态光散射（MALS-DLS）的检测方法相结合，以支持大卫S. Hage，Chin Li（巴里）Cheung，Edward Harris，Wei Niu，and Angela K.帕尼尔该仪器促进了该机构在化学，生物化学，化学和生物分子工程以及生物系统工程领域的研究。SEC基于分子从填充柱的洗脱将分子分离为大小的函数。FFF是一种通用的分离技术，可应用于大分子和纳米颗粒。MALS-DLS随后用于在线测量散射光中的时间依赖性波动，这与分子扩散系数及其尺寸分布有关。该工具增强了许多部门各级学生的教育，研究和教学努力，并提供了在附近机构使用的无障碍环境。该工具在研究培训机会中得到利用，影响到从纳米材料和聚合物到生物医学研究和环境研究的许多领域。SEC/FFF-MALS-DLS奖项旨在加强各个层面的研究和教育，特别是在化学、高分子科学、生物化学、化学和生物分子工程以及生物系统工程等领域。研究重点是环境和化学研究，旨在检查药物在水中的活性及其与溶解有机物的结合，以及表征降解水中纳米塑料和微塑料的新方法。该仪器还用于分离尺寸特异性形式的复合碳水化合物透明质酸以检查其生物活性，表征和分离重组蛋白及其结构域以用于酶工程，该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准。

This award is jointly supported by the Major Research Instrumentation, the Established Program to Stimulate Competitive Research (EPSCoR), and the Chemistry Research Instrumentation Programs. The University of Nebraska – Lincoln is acquiring an instrumental platform that combines the chemical separation methods of size-exclusion chromatography (SEC) and field-flow fractionation (FFF) with the detection methods of multi-angle light scattering and dynamic light scattering (MALS-DLS) to support the research of Professor David S. Hage, Chin Li (Barry) Cheung, Edward Harris, Wei Niu, and Angela K. Pannier. This instrument facilitates research in the areas of chemistry, biochemistry, chemical and biomolecular engineering, and biological systems engineering at this institution. SEC separates molecules as a function of size based on their elution from a packed column. FFF is a versatile separation technique that can be applied to macromolecules and nanoparticles. MALS-DLS are subsequently used, in-line, to measure time-dependent fluctuations in scattered light, which is related to molecular diffusion coefficients and their size distributions. This instrument enhances the educational, research, and teaching efforts of students at all levels in many departments as well as provides accessibility for use at nearby institutions. The instrument is leveraged in research training opportunities that impacts many areas spanning from nanomaterials and polymers to biomedical research and environmental studies. Students, local industry and others gain hands-on experience with the instrument through its integration in various program.The award of the SEC/FFF-MALS-DLS is aimed at enhancing research and education at all levels, especially in areas such as chemistry, polymer science, biochemistry, chemical and biomolecular engineering, and biological systems engineering. Research focuses on environmental and chemical research aimed at examining the activity of pharmaceuticals in water and their binding with dissolved organic matter, as well as the characterization of new methods for the degradation of nanoplastics and microplastics in water. The instrument is also used to isolate size-specific forms of the complex carbohydrate hyaluronan for examining their biological activities, to characterize and separate recombinant proteins and their domains for enzyme engineering, and to isolate and characterize synthetic nanoparticles and biological vesicles for use in biomedical research.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.