CAS: Ultrafast Affinity Extraction - Fundamental Studies and Use in Environmental Applications

CAS：超快亲和提取 - 基础研究和在环境应用中的使用

基本信息

批准号：
2108881
负责人：
David Hage
金额：
$ 40万
依托单位：
University of Nebraska-Lincoln
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2021
资助国家：
美国
起止时间：
2021-07-15 至 2025-06-30
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2108881&HistoricalAwards=false
关键词：
CAS Ultrafast Affinity Extraction Fundamental

项目摘要

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, Professor David Hage and his group at the University of Nebraska-Lincoln are working to advance our ability to monitor chemicals in the environment, including chemicals of importance to national health. Specifically, new microscale methods are being developed. These methods make it possible to study and analyze chemical systems that cannot be easily examined by other techniques, providing new information on the behavior, transport, and biological activity of pharmaceuticals and other man-made agents. Students working with these innovative methods are receiving interdisciplinary training in chemical analysis, chemical separations, and environmental analysis. Curricular materials on microscale separation methods and environmental analysis are also being developed by Professor Hage for the further training of students within and beyond the field of chemical separations.The overall goal of this project is to develop and utilize new high-performance separation methods based on ultrafast affinity extraction to characterize chemical interactions of species with weak-to-moderate binding strengths. These interactions are important in determining the transport and activity of many man-made agents in the environment and in biological systems. The approach begins with simulations and analysis of model systems to identify suitable ultrafast affinity extraction conditions for measuring the non-bound chemical fractions and for use in binding or rate studies. The derived conditions are then applied to examine the binding in “real” applications (e.g., binding of common pharmaceuticals with dissolved organic matter, such as humic acids, found in water). Finally, results are used to develop new formats of ultrafast affinity extraction to increase the information content and capabilities of this method in binding and rate studies.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学分部的化学测量和成像计划的支持下，内布拉斯加州林肯大学的戴维·哈奇（David Hage）及其小组正在努力提高我们监测环境中化学物质的能力，包括对国家健康的重要性。具体而言，正在开发新的微观方法。这些方法使研究和分析无法通过其他技术轻松检查的化学系统，从而提供有关药物和其他人造剂的行为，运输和生物活性的新信息。使用这些创新方法的学生正在接受化学分析，化学分离和环境分析方面的跨学科培训。 Hage教授还开发了有关微观分离方法和环境分析的课程材料，以进一步培训化学分离领域内外的学生。该项目的总体目标是开发和利用基于超快亲和力提取的新的高性能分离方法，以表征具有弱至中度结合强度的物种化学相互作用。这些相互作用对于确定许多人造药物在环境和生物系统中的运输和活动很重要。该方法始于模拟系统的模拟和分析，以识别用于测量非结合化学部分和用于结合或速率研究的合适超快的亲和力提取条件。然后将衍生条件应用于“真实”应用中的结合（例如，在水中发现的溶解有机物（例如腐殖酸），常见药物与溶解有机物的结合）。最后，结果用于开发超快亲和力提取的新格式，以提高这种方法在结合和速率研究中的信息内容和能力。该奖项反映了NSF的法定任务，并被认为是通过基金会的智力优点和更广泛的影响来通过评估来获得支持的。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Evaluation of microcolumn stability in ultrafast affinity extraction for binding and rate studies.

DOI：
10.1016/j.jchromb.2021.123047
发表时间：
2021-12-15
期刊：
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
影响因子：
0
作者：
Iftekhar S;Hage DS
通讯作者：
Hage DS

Recent Advances in Supramolecular Affinity Separations: Affinity Chromatography and Related Methods.

DOI：
10.1201/9781003223405-1
发表时间：
2021
期刊：
Advances in chromatography
影响因子：
0
作者：
通讯作者：

Analysis of the binding of warfarin to glyoxal- and methylglyoxal-modified human serum albumin by ultrafast affinity extraction.

DOI：
10.1016/j.jchromb.2022.123500
发表时间：
2022-11-15
期刊：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
影响因子：
3
作者：
Iftekhar, Sazia;Li, Zhao;Tao, Pingyang;Poddar, Saumen;Hage, David S.
通讯作者：
Hage, David S.

Approaches for the detection and analysis of antidrug antibodies to biopharmaceuticals: A review.