NSF East Asia and Pacific Summer Institute (EAPSI) for FY 2013 in China

2013 财年 NSF 东亚及太平洋暑期学院 (EAPSI) 在中国

• 批准号: 1316974
• 负责人: Douglas Smith
• 金额: $ 0.51万
• 依托单位: Smith Douglas C
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2014-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1316974&HistoricalAwards=false
• 关键词: NSF; East; Asia; Pacific; Summer

This action funds Douglas Smith of Michigan Technological University to conduct a research project in the Mathematical and Physical Sciences area during the summer of 2013 at Beijing Institute of Technology in Beijing, China. The project title is "Nanoparticle-based Fluorescent Sensors for Measurement of Acidic Intracellular pH in Living Cells." The host scientist is Dr. Si-ping Pang.Intracellular pH plays many critical roles in cell, enzyme and tissue activities, including proliferation and apoptosis, ion transport, endocytosis, and muscle contraction. Cellular dysfunction is often associated with abnormal pH values in organelles (mitochondria, lysosomes, etc.). Monitoring pH changes inside living cells is also important for studying cellular internalization pathways, such as phagocytosis, endocytosis, and receptor ligand internalization. The Fellow synthesized a series of new fluorescent sensors in his home lab (Michigan Tech. Univ.). Several features of the sensors (absence of cytotoxicity, high cell permeability, and long-lived intracellular fluorescence) make the pH sensors superior candidates for examining intracellular pH alterations in intact cells. Other commercially available pH sensors do not exhibit these features. Accordingly, the sensors offer novel experimental opportunities for live-imaging in intact cells over multiple time points (hours), and thus add a new tool in understanding intracellular alterations in normal and cancerous cell growth. The easily accessible terminal alkyne/ azido "clickable" function groups of these sensors offer the possibility of rapidly constructing sensor molecule libraries using "click" chemistry. This project exploits this approach to prepare multiple nanoparticle-based fluorescent sensors. Broader impacts of an EAPSI fellowship include providing the Fellow a first-hand research experience outside the U.S.; an introduction to the science, science policy, and scientific infrastructure of the respective location; and an orientation to the society, culture and language. These activities meet the NSF goal to educate for international collaborations early in the career of its scientists, engineers, and educators, thus ensuring a globally aware U.S. scientific workforce.

这项行动为密歇根技术大学的道格拉斯·史密斯（Douglas Smith）提供了资金，在2013年夏季在中国北京的北京技术研究院开展了数学和物理科学领域的研究项目。 该项目的标题是“基于纳米颗粒的荧光传感器，用于测量活细胞中酸性细胞内pH值”。宿主科学家是Si-ping Pang.Introacellular pH博士在细胞，酶和组织活性中起许多关键作用，包括增殖和凋亡，离子转运，内吞作用和肌肉收缩。细胞功能障碍通常与细胞器（线粒体，溶酶体等）中的pH值异常有关。监测活细胞内部的pH变化对于研究细胞内部化途径，例如吞噬作用，内吞作用和受体配体内在化也很重要。 该研究员在他的家庭实验室（密歇根理工大学）合成了一系列新的荧光传感器。传感器的几种特征（缺乏细胞毒性，高细胞渗透率和长寿命的细胞内荧光）使pH传感器具有出色的候选者，用于检查完整细胞中细胞内pH的改变。其他市售的pH传感器没有显示这些功能。因此，这些传感器为完整细胞在多个时间点（小时）的完整细胞中提供了新的实验机会，因此为理解正常和癌细胞生长的细胞内改变添加了一种新工具。 这些传感器的易于访问的端子炔烃/方济多“可单击”功能组提供了使用“ Click” Chemistry快速构建传感器分子库的可能性。 该项目利用这种方法来制备多个基于纳米颗粒的荧光传感器。 EAPSI奖学金的更广泛的影响包括为这位美国境外的第一手研究经验提供；各自位置的科学，科学政策和科学基础设施的简介；以及社会，文化和语言的方向。 这些活动符合NSF的目标，以在其科学家，工程师和教育工作者的职业生涯的早期对国际合作进行教育，从而确保全球意识的美国科学劳动力。