Comprehensive genetic dissection of a model highly complex trait in budding yeast

芽殖酵母高度复杂性状模型的综合遗传解析

• 批准号: 1330874
• 负责人: Ian Ehrenreich
• 金额: $ 51万
• 依托单位: University of Southern California
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-12-01 至 2016-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1330874&HistoricalAwards=false
• 关键词: Comprehensive; genetic; dissection; model; highly

Elucidating the connection between genotype and phenotype is critical to achieving new heights in agriculture, evolutionary biology, and medicine. In this project, our goal is to help characterize basic principles underlying the genotype-phenotype map by comprehensively dissecting a genetically complex trait in a "simple" model organism. Specifically, we will extend our ongoing research on variability in susceptibility to DNA damage in Saccharomyces cerevisiae. We will use statistically powerful and high-resolution genetic mapping experiments to identify the dozens of genetic variants that cause differences in DNA damage tolerance among three strains. We will then study how these genes interact and exert their effects using genetic experiments and functional assays. Our work should produce one of the most detailed genetic characterizations of a highly complex trait to date, and could also provide new insights into how cells detect and repair DNA damage.As part of our broader impacts on society, we will use the proposed research to train multiple PhD, undergraduate, and high school students. We will also work with Rebecca Cooper, who instructs four sections of AP Biology at Burbank High School, to develop multiple lab practicals in which we use heritable trait variation in yeast to help teach biology to high school students. Additional interactions with Ms. Cooper?s class will involve talking with students about career opportunities in science, as well as discussing the many impacts of biological research on everyday life. Lastly, we will use the proposed work as a vehicle to expose minorities to research through the USC GREU program.

阐明基因型和表型之间的联系对于在农业、进化生物学和医学上达到新的高度至关重要。在这个项目中，我们的目标是通过全面剖析“简单”模式生物中的遗传复杂性状来帮助描述基因型-表型图谱的基本原理。具体来说，我们将扩大我们正在进行的研究在酿酒酵母DNA损伤的易感性的变化。我们将使用统计学上强大的高分辨率遗传作图实验来确定导致三种菌株之间DNA损伤耐受性差异的数十种遗传变异。然后，我们将研究这些基因如何相互作用，并利用遗传实验和功能测定发挥其作用。我们的工作将产生迄今为止最详细的高度复杂性状的遗传特征之一，也可以为细胞如何检测和修复DNA损伤提供新的见解。作为我们对社会更广泛影响的一部分，我们将利用拟议的研究来培训多名博士，本科生和高中生。我们还将与丽贝卡库珀，谁指导AP生物学在伯班克高中的四个部分，开发多个实验室实践中，我们使用酵母中的遗传性状变异，以帮助教生物学高中学生。与库珀女士的其他互动这堂课将涉及与学生谈论科学方面的职业机会，以及讨论生物研究对日常生活的许多影响。最后，我们将利用拟议的工作作为一种工具，通过南加州大学GREU计划，使少数群体接触研究。