Interplay of Transcriptional, Translational Regulatory Mechanisms and Kinetics of an Environmental Response

转录、翻译调节机制和环境反应动力学的相互作用

• 批准号: 1330912
• 负责人: Nitin Baliga
• 金额: $ 120万
• 依托单位: Institute for Systems Biology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-15 至 2017-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1330912&HistoricalAwards=false
• 关键词: Interplay; Transcriptional; Translational; Regulatory; Mechanisms

INTELLECTUAL MERIT: Environmental factors change with reproducible characteristics. While changes in oxygen can happen within seconds to minutes, temperature changes occur at a much slower pace over hours to days. Regulatory networks in an organism are tuned through evolution to elicit a response that matches the characteristics of a given environmental factor (e.g. a rapid response to fast changing factors). The overarching hypothesis of this project is that the relative use of regulation at different stages of information processing (transcriptional, post-transcriptional, translational and post-translational) is dictated by the necessary characteristics of an environmental response. In order to test this hypothesis under varying environmental conditions, changes in synthesis and degradation rates will be measured for all transcripts and proteins in strains of Halobacterium salinarum. Building in this proof-of-concept, the research project will systematically investigate interplay across different levels of regulation in the context of specific environmental responses.BROADER IMPACTS:Resources: This research will generate fundamental principles of integration across different levels of regulation, and also generate innovative computational and experimental methodology that will be published as well-documented, open-source data and software. Education: Rapid advances in interdisciplinary science are widening the gap in the way biology is taught and how it is practiced. An award winning high school (HS) education program in the lab is bridging this divide through inquiry-based teaching practices at the grassroots. This ten-year-old program is centered on summer internships for students of disadvantaged backgrounds who are encouraged to apply through numerous partnerships including programs for inner city youth and a UW-based program for advancement of women in science. Educational kits developed through this program have been aligned with state standards, iteratively improved through classroom pilots, and disseminated through professional teacher development to 14 states across the US. In addition to training postdoctoral fellows in systems biology, this project will also develop inquiry-driven, standards-based, HS educational materials. Developed together with local science educators, this novel curriculum module has been requested by teachers and will help students internalize the concepts and methods for using systems biology to understand cellular dynamics in the context of environmental change. These curricula will be aligned with the new Next Generation Science Standards and regularly assess its impact on student learning and career choices through a rigorous evaluation program.

智力优势：环境因素的变化具有可复制的特征。虽然氧气的变化可以在几秒到几分钟内发生，但温度变化的速度要慢得多，需要数小时到数天。生物体中的调节网络通过进化进行调整，以引发与给定环境因素特征相匹配的反应（例如，对快速变化的因素的快速反应）。这个项目的首要假设是，在信息处理的不同阶段（转录、转录后、翻译和翻译后），调控的相对使用是由环境反应的必要特征决定的。为了在不同的环境条件下验证这一假设，将测量盐盐杆菌菌株中所有转录本和蛋白质的合成和降解率的变化。在此概念验证的基础上，该研究项目将系统地调查在特定环境响应的背景下不同级别的监管之间的相互作用。更广泛的影响：资源：这项研究将产生跨不同级别监管整合的基本原则，并产生创新的计算和实验方法，这些方法将作为记录良好的开源数据和软件发布。教育：跨学科科学的快速发展正在拉大生物学的教学方式和实践方式的差距。一个屡获殊荣的高中（HS）教育项目在实验室正在弥合这种鸿沟，通过探究为基础的教学实践在基层。这个已有十年历史的项目主要是为弱势背景的学生提供暑期实习机会，鼓励他们通过许多合作伙伴关系申请，其中包括针对内城青年的项目和一个以威斯康星大学为基础的促进女性在科学领域发展的项目。通过该项目开发的教育工具包已与各州标准保持一致，通过课堂试点不断改进，并通过专业教师培训向美国14个州传播。除了培养系统生物学方面的博士后外，该项目还将开发探究驱动的、基于标准的HS教育材料。与当地科学教育工作者共同开发，这一新颖的课程模块应教师的要求，将帮助学生内化使用系统生物学的概念和方法，以了解环境变化背景下的细胞动力学。这些课程将与新的下一代科学标准保持一致，并通过严格的评估计划定期评估其对学生学习和职业选择的影响。