Defining transcriptional and post-translational regulatory networks in retrogradw stress signaling

植物持续受到许多生物和非生物胁迫的挑战。为了应对，它们进化出了一个复杂的机制网络来连接这些应激反应途径。确定这些反应途径的主要调控因子是为开发广谱抗逆性植物提供机会的基础。最近，人们发现一种新的质体产生的胁迫特异性信号代谢物甲基erythritol cyclodiphosphate (MEcPP)，作为一个感觉中枢，同步多个调控步骤，这些步骤是植物对环境扰动反应的关键。这一发现为鉴定一般应激反应调节因子建立了一个独特的平台。该项目的目的有两个方面：1)确定参与MEcPP信号级联的分子和生化成分，该信号级联将质体感知的应激信号传递到负责表达生物和非生物应激反应关键调节因子的中心枢纽。2)鉴定可能连接植物适应多种环境胁迫所需的感觉系统综合网络的细胞相互作用组。因此，该项目提供了一个独特的机会来明确识别和功能表征植物对生物和非生物胁迫的适应性反应关键的一般胁迫基因簇的集合。该项目提供了一个多维教育平台，指导学者，特别是代表性不足的大学和高中学生进行垂直整合的最先进的跨学科翻译研究。该项目的成果将每天储存在部门的服务器上，并通过出版物和公开演讲广泛传播，所有开发的工具都将提供给社会各界。该项目的全球影响是开发新的目标，以产生广谱抗逆性作物，从而减轻气候变化和全球粮食安全造成的压力。

Plants are persistently challenged with numerous biotic and abiotic stresses. To cope, they have evolved an intricate network of mechanisms to link these stress response pathways. Identification of master regulators that prime these response pathways is fundamental to providing opportunities for developing broad-spectrum stress-tolerant plants. Recently, it was discovered that a novel plastid-produced stress-specific signaling metabolite, methylerythritol cyclodiphosphate (MEcPP), serves as a sensory hub that synchronizes multiple regulatory steps that are key to plant responses to environmental perturbations. This discovery has led to construction of a unique platform for identification of the general stress response regulators. The aims of this project are two-fold: 1) to identify the molecular and biochemical components engaged in the MEcPP signaling cascade that relay plastid-perceived stress signals to a central hub responsible for expression of key regulators of biotic and abiotic stress responses. 2) to identify the cellular interactomes that might connect the integrative network of sensory systems required for plants adaptive responses to multiple environmental stresses. As such, this project provides a unique opportunity to unambiguously identify and functionally characterize an ensemble of a general stress cluster of genes key to plant adaptive responses to biotic and abiotic stresses. This project provides a multidimensional educational platform for mentoring scholars and particularly underrepresented college and high school students in a vertically integrated state-of-the-art interdisciplinary translational research. The results of this project will be stored daily on the departmental server and widely disseminated through publications and public presentations, and all of the tools developed will be made available to the community. The global impact of this project is the development of new targets for generation of broad-spectrum stress-tolerant crops, thereby alleviating pressures caused by climate change and global food security.