Defining transcriptional and post-translational regulatory networks in retrogradw stress signaling

植物持续受到许多生物和非生物胁迫的挑战。 为了科普，它们进化出了一个复杂的机制网络来连接这些压力反应途径。确定这些反应途径的主要调控因子对于为开发广谱抗逆植物提供机会至关重要。最近，人们发现一种新的质体产生的胁迫特异性信号代谢物，甲基环二磷酸（MEcPP），作为一个感觉枢纽，包括多个调节步骤，是植物对环境扰动的反应的关键。 这一发现导致了一个独特的平台，用于识别一般的应激反应调节剂的建设。该项目的目的是双重的：1）鉴定参与MEcPP信号级联的分子和生物化学组分，所述MEcPP信号级联将质体感知的胁迫信号中继到负责生物和非生物胁迫响应的关键调节剂的表达的中心枢纽。2)识别可能连接植物对多种环境胁迫的适应性反应所需的感觉系统的整合网络的细胞相互作用组。因此，该项目提供了一个独特的机会，明确地确定和功能特征的一个整体的一般应力基因簇的关键植物适应性反应的生物和非生物胁迫。 该项目为指导学者，特别是代表性不足的大学和高中学生提供了一个多维的教育平台，在垂直整合的最先进的跨学科翻译研究中。 该项目的结果将每天储存在该部的服务器上，并通过出版物和公开介绍广泛传播，开发的所有工具将提供给社区。 该项目的全球影响是为广谱抗逆作物的产生制定新的目标，从而减轻气候变化和全球粮食安全造成的压力。

Plants are persistently challenged with numerous biotic and abiotic stresses. To cope, they have evolved an intricate network of mechanisms to link these stress response pathways. Identification of master regulators that prime these response pathways is fundamental to providing opportunities for developing broad-spectrum stress-tolerant plants. Recently, it was discovered that a novel plastid-produced stress-specific signaling metabolite, methylerythritol cyclodiphosphate (MEcPP), serves as a sensory hub that synchronizes multiple regulatory steps that are key to plant responses to environmental perturbations. This discovery has led to construction of a unique platform for identification of the general stress response regulators. The aims of this project are two-fold: 1) to identify the molecular and biochemical components engaged in the MEcPP signaling cascade that relay plastid-perceived stress signals to a central hub responsible for expression of key regulators of biotic and abiotic stress responses. 2) to identify the cellular interactomes that might connect the integrative network of sensory systems required for plants adaptive responses to multiple environmental stresses. As such, this project provides a unique opportunity to unambiguously identify and functionally characterize an ensemble of a general stress cluster of genes key to plant adaptive responses to biotic and abiotic stresses. This project provides a multidimensional educational platform for mentoring scholars and particularly underrepresented college and high school students in a vertically integrated state-of-the-art interdisciplinary translational research. The results of this project will be stored daily on the departmental server and widely disseminated through publications and public presentations, and all of the tools developed will be made available to the community. The global impact of this project is the development of new targets for generation of broad-spectrum stress-tolerant crops, thereby alleviating pressures caused by climate change and global food security.